Acne is a chronic inflammatory skin disease that often occurs with anaerobic Propionibacterium acnes (P. acnes). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic conditions and promote P. acnes growth. In this study, gelatin/chitosan (GC) bilayer patches were prepared at different temperatures that included room temperature (RT), −20 °C/RT, and −80 °C/RT. The most promising GC bilayer patch (−80 °C /RT) contained a dense upper layer for protection from bacteria and infection and a porous lower layer for absorbing pus and fluids from pimples. The anti-acne bilayer patch was loaded with Cortex Phellodendri amurensis (PA) and Centella asiatica (CA) extracts. PA extract could inhibit the growth of P. acnes and CA extract was reported to improve wound healing and reduce scar formation. Moreover, the water retention rate, weight loss rate, antibacterial activity, and in vitro cytotoxicity of the patches were investigated. The porous structure of the patches promoted water retention and contributed to absorbing the exudate when used on open acne wounds. The GC bilayer patches loaded with PA/CA extracts were demonstrated to inhibit the growth of P. acnes, and accelerate the skin fibroblast cell viability. Based on their activities and characteristics, the GC bilayer patches with PA/CA extract prepared at −80 °C/RT obtain the potential for the application of acne spot treatment.

The artificial gastric-juice digestive method was used to examine 250 preserved clams, 125 living Corbicula fluminea samples, and 53 preserved fish that were all bought randomly from markets. We did not observe any metacercariae in the products bought from traditional markets. However, this does not indicate that preserved aquatic products can be sold without being cooked because, we observed that self-infected metacercariae survive for 5 minutes after being digested in fish bodies can still at a temperature of 50°C, but survive for only 1 minute when the temperature rises to 80°C. In an experimental infection study, we determined that Corbicula fluminea was not infected with xiphidiocercariae, but the fins of Opsariichthys pachycephalus can be infected and experimental metacercariae were observed. Austropeplea ollula was determined to be the first intermediate host of the xiphidiocercaria parasite, and Opsariichthys pachycephalus is a suitable second intermediate host of trematodes. Therefore, raw or insufficiently cooked, they pass down parasitic diseases.