We encapsulated MSZ in zein nanoparticles (NP-ZN) using a desolvation method followed by drying in a mini spray dryer. These nanoparticles exhibited a size of 266.6 ± 52 nm, IPD of 0.14 ± 1.1 and zeta potential of −36.4 ± 1.5 mV, suggesting colloidal stability. Quantification using HPLC showed a drug-loaded of 43.8 µg/mg. SEM demonstrated a spherical morphology with a size variation from 220 to 400 nm. A FTIR analysis did not show drug spectra in the NPs in relation to the physical mixture, which suggests drug encapsulation without changing its chemical structure. A TGA analysis showed thermal stability up to 300 °C. In vitro release studies demonstrated gastroresistance and a sustained drug release at pH 7.4 (97.67 ± 0.32%) in 120 h. The kinetic model used for the release of MSZ from the NP-ZN in a pH 1.2 medium was the Fickian diffusion, in a pH 6.8 medium it was the Peppas–Sahlin model with the polymeric relaxation mechanism and in a pH 7.4 medium it was the Korsmeyer–Peppas model with the Fickian release mechanism, or “Case I”. An in vitro cytotoxicity study in the CT26.WT cell line showed no basal cytotoxicity up to 500 μg/mL. The NP-ZN showed to be a promising vector for the sustained release of MSZ in the colon by oral route.

The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). Mus musculus virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 μg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.

Introduction: Inflammatory Bowel Disease (IBD) are diseases characterized by intestinal inflammation, divided into Crohn's Disease (CD) and Ulcerative Retrocolitis (UC). The cause is still unknown. There are a variety of studies that show the beneficial and potential effect of phytocannabinoids on Inflammatory Bowel Disease. Objective: To evaluate the beneficial effect of Cannabis sativa-derived metabolites in the treatment of IBD, associated with the use of nanotechnology in order to enhance this effect, through a systematic review study. Methodology: This systematic review of the literature of pre-clinical and clinical trials was carried out in the following databases: PUBMED, Science Direct and Scopus from 2012 to 2022. Some combinations of terms were used, such as inflammatory bowel diseases, cannabinoids, cannabidiol and nanotechnology. Results: A total of 418 articles were found in Pubmed, 80 articles in Science Direct and 443 in Scopus. In the end, 6 articles in Pubmed, 10 in Science Direct and 6 in Scopus were considered eligible for analysis according to the established inclusion and exclusion criteria. Discussion: In the analyzed studies, it was noticed that phytocannabinoids represent a promising alternative to treat IBD. Despite the great expectations surrounding these molecules, their physicochemical characteristics represent a significant limitation for their application in the treatment of IBD and other diseases. Therefore, nanotechnology proved to be a way to overcome these problems. Conclusion: In view of all the above, it can be inferred that metabolites derived from Cannabis sativa have great potential for the treatment of inflammatory bowel diseases.

Introdução: A ocorrência de óbitos por intoxicação com medicamentos tem sido considerada um dos agravos de saúde pública. Objetivo: Descrever as principais determinantes nas intoxicações por fármacos na cidade de Teresina-PI. Metodologia: Pesquisa ecológica, descritiva, quantitativa e de base populacional, com estudo de envenenamento por fármacos notificados na cidade de Teresina-PI, de 2010 a 2019 do Ministério da Saúde/SINAN. Foram coletados os seguintes dados: sexo, etnia, escolaridade, faixa etária, evolução clínica, classificação final, circunstância, critério de confirmação e o município de residência no Piauí. 2019.Resultados e discussão: No período analisado foi registrado 2.229 ocorrências, sendo preponderante a etnia parda (41,36%), escolaridade com ensino médio completo (12,43%), faixa etária de 20-39 (43,43%) e sexo feminino (73,44%). Já no tipo de exposição a aguda- crônica ocorreu em 1287 registros (57,74%), e o critério de confirmação foi clínico (64,56%). A circunstância mais frequente foi tentativa de suicídio (71,02%) e o desfecho clinico final foi cura com ausência de dano (59,22%). Conclusão: Constatou-se que na cidade de Teresina-PI ocorreu um aumento das notificações, esse fato pode ser atribuído ao padrão de consumo de fármacos. De modo geral, a Vigilância em Saúde deve priorizar a sensibilidade profissional para obter mais ações educativas, preventivas e intersetoriais, para assegurar o aprimoramento das políticas de vigilância sanitária.