Leaf area index (LAI) is a crucial biophysical variable for agroecosystems monitoring. Conventional vegetation indices (VIs) based on red and near infrared regions of the electromagnetic spectrum, such as the normalized difference vegetation index (NDVI), are commonly used to estimate the LAI. However, these indices commonly saturate at moderate-to-dense canopies (e.g., NDVI saturates when LAI exceeds three). Modified VIs have then been proposed to replace the typical red/green spectral region with the red-edge spectral region. One significant and often ignored aspect of this modification is that the reflectance in the red-edge spectral region is comparatively sensitive to chlorophyll content which is highly variable between different crops and different phenological states. In this study, three improved indices are proposed combining reflectance both in the red and red-edge spectral regions into the NDVI, the modified simple ratio index (MSR), and the green chlorophyll index (CI_green) formula. These improved indices are termed NDVI_{red& RE} (red and red-edge NDVI), MS_{Rred& RE} (red and red-edge MSR index), and CI_{red& RE} (red and red-edge CI). The indices were tested using RapidEye images and in-situ data from campaigns at Maccarese Farm (Central Rome, Italy), in which four crop types at four different growth stages were measured. We investigated the predictive power of nine VIs for crop LAI estimation, including NDVI, MSR, and CIgreen; the red-edge modified indices: NDVI_Red-edge, MSR_Red-edge, and CI_Red-edge (generally represented by VIRed-edge); and the newly improved indices: NDVI_{red& RE}, MSR_{red& RE}, and CI_{red& RE} (generally represented by VI_{red& RE}). The results show that VI_{red& RE} improves the coefficient of determination (R²) for LAI estimation by 10% in comparison to VI_Red-edge. The newly improved indices prove to be the powerful alternatives for the LAI estimation of crops with wide chlorophyll range, and may provide valuable information for satellites equipped with red-edge channels (such as Sentinel-2) when applied to precision agriculture.

This study focuses on the comparison of hybrid methods of estimation of biophysical variables such as leaf area index (LAI), leaf chlorophyll content (LCC), fraction of absorbed photosynthetically active radiation (FAPAR), fraction of vegetation cover (FVC), and canopy chlorophyll content (CCC) from Sentinel-2 satellite data. Different machine learning algorithms were trained with simulated spectra generated by the physically-based radiative transfer model PROSAIL and subsequently applied to Sentinel-2 reflectance spectra. The algorithms were assessed against a standard operational approach, i.e., the European Space Agency (ESA) Sentinel Application Platform (SNAP) toolbox, based on neural networks. Since kernel-based algorithms have a heavy computational cost when trained with large datasets, an active learning (AL) strategy was explored to try to alleviate this issue. Validation was carried out using ground data from two study sites: one in Shunyi (China) and the other in Maccarese (Italy). In general, the performance of the algorithms was consistent for the two study sites, though a different level of accuracy was found between the two sites, possibly due to slightly different ground sampling protocols and the range and variability of the values of the biophysical variables in the two ground datasets. For LAI estimation, the best ground validation results were obtained for both sites using least squares linear regression (LSLR) and partial least squares regression, with the best performances values of R² of 0.78, rott mean squared error (RMSE) of 0.68 m² m⁻² and a relative RMSE (RRMSE) of 19.48% obtained in the Maccarese site with LSLR. The best results for LCC were obtained using Random Forest Tree Bagger (RFTB) and Bagging Trees (BagT) with the best performances obtained in Maccarese using RFTB (R² = 0.26, RMSE = 8.88 μg cm⁻², RRMSE = 17.43%). Gaussian Process Regression (GPR) was the best algorithm for all variables only in the cross-validation phase, but not in the ground validation, where it ranked as the best only for FVC in Maccarese (R² = 0.90, RMSE = 0.08, RRMSE = 9.86%). It was found that the AL strategy was more efficient than the random selection of samples for training the GPR algorithm.

SUMMARY: The widespread adoption by agronomists and researchers of handheld leaf chlorophyll meters stimulates enquiries on instrumental calibration issues, given the necessity, for some applications, of inferring actual chlorophyll concentrations from the readings provided. This is especially required for recently developed and more innovative devices such as the Dualex (Force-A, France), which unlike the more common SPAD-502 (Minolta, Japan) has not undergone extensive (published) calibration tests. Additionally, devices for spectral reflectance measurements are also becoming increasingly available. In the present paper, the calibration of SPAD on maize (Zea mays L.) and of Dualex on winter wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), horse bean (Vicia faba L.) and maize, was compared to spectral reflectance indices and full spectral information (400–2500 nm) acquired by a spectroradiometer (ASD FieldSpec) equipped with a contact probe and leaf clip. Full spectral data were exploited using partial least squares regression (PLSR). The measurements were performed in the field at Maccarese (Central Italy) in 2012, gathering a specific experimental dataset. The calibration models obtained on experimental data for SPAD (on maize) and Dualex (on four crops) showed intermediate or high estimation accuracy with root-mean-square error (RMSE) values ranging between 7 and 11 μg/cm² depending on the species. These results were slightly better than those achieved using spectral reflectance indices, which were inferior though to those provided by PLSR using full spectral resolution. A synthetic database, generated by the physically based PROSPECT model, simulating hemispherical leaf reflectance and transmittance, was used to compare the performances of the reflectance indices and the chlorophyll meters for a wider range of leaf properties. The results confirmed the substantial equivalence of reflectance-based and transmittance-based (i.e. simulated SPAD and Dualex) indices and the advantage of exploiting the full spectral information, e.g. through PLSR, if available.

The PRISMA satellite is equipped with an advanced hyperspectral Earth observation technology capable of improving the accuracy of quantitative estimation of bio-geophysical variables in various Earth Science Applications and in particular for soil science. The purpose of this research was to evaluate the ability of the PRISMA hyperspectral imager to estimate topsoil properties (i.e., organic carbon, clay, sand, silt), in comparison with current satellite multispectral sensors. To investigate this expectation, a test was carried out using topsoil data collected in Italy following two approaches. Firstly, PRISMA, Sentinel-2 and Landsat 8 spectral simulated datasets were obtained from the spectral resampling of a laboratory soil library. Subsequently, bare soil reflectance data were obtained from two experimental areas in Italy, using real satellites images, at dates close to each other. The estimation models of soil properties were calibrated employing both Partial Least Square Regression and Cubist Regression algorithms. The results of the study revealed that the best accuracies in retrieving topsoil properties were obtained by PRISMA data, using both laboratory and real datasets. Indeed, the resampled spectra of the hyperspectral imager provided the best Ratio of Performance to Inter-Quartile distance (RPIQ) for clay (4.87), sand (3.80), and organic carbon (2.59) estimation, for the spectral soil library datasets. For the bare soil reflectance obtained from real satellite imagery, a higher level of prediction accuracy was obtained from PRISMA data, with RPIQ ± SE values of 2.32 ± 0.07 for clay, 3.85 ± 0.19 for silt, and 3.51 ± 0.16 for soil organic carbon. The results for the PRISMA hyperspectral satellite imagery with the Cubist Regression provided the best performance in the prediction of silt, sand, clay and SOC. The same variables were better estimated using PLSR models in the case of the resampled hyperspectral data. The statistical accuracy in the retrieval of SOC from real and resampled PRISMA data revealed the potential of the actual hyperspectral satellite. The results supported the expected good ability of the PRISMA imager to estimate topsoil properties.