In addition to properly balancing nutritional value in accordance with the needs of a dog, estimating the microbiological quality of dog food is crucial in providing healthy and safe foods. The aim of this study was to examine the quality of dry food for adult dogs, with particular reference to: (1) evaluating the nutritional value and compliance with nutritional guidelines for dogs, (2) comparing the nutritional value of dog foods, with particular emphasis on the division into cereal and cereal-free foods, and (3) evaluating their microbiological safety. All thirty-six evaluated dry dog foods met the minimum European Pet Food Industry FEDIAF requirement for total protein and fat content. The total aerobic microbial count in the analyzed dry dog foods ranged from 2.7 × 10² to above 3.0 × 10⁷ cfu/g. In five (14%) dog foods the presence of staphylococci was detected; however, coagulase positive Staphylococcus (CPS) was not found. Mold presence was reported in one cereal-free dog food and in six cereal foods. In none of the analyzed foods Enterobacteriaceae were found, including coliforms, Escherichia coli and Salmonella spp. Bacteria of the genus Listeria and Clostridium as well as yeasts were also not detected. In conclusion, the evaluated dry dog foods had varied microbiological quality. The detected number of microorganisms may have some implications for long-term consumption of contaminated food. The lack of European Commission standards regarding the permissible amounts of microorganisms in pet food may result in insufficient quality control of these products.

The main objectives of this study were to isolate bacteria from soil chronically contaminated with polycyclic aromatic hydrocarbons (PAHs), develop an autochthonous microbial consortium, and evaluate its ability to degrade PAHs in their native contaminated soil. Strains with the best bioremediation potential were selected during the multi-stage isolation process. Moreover, to choose bacteria with the highest bioremediation potential, the presence of PAH-degrading genes (pahE) was confirmed and the following tests were performed: tolerance to heavy metals, antagonistic behavior, phytotoxicity, and antimicrobial susceptibility. In vitro degradation of hydrocarbons led to the reduction of the total PAH content by 93.5% after the first day of incubation and by 99.22% after the eighth day. Bioremediation experiment conducted in situ in the contaminated area resulted in the average reduction of the total PAH concentration by 33.3% after 5 months and by over 72% after 13 months, compared to the concentration recorded before the intervention. Therefore, this study implicates that the development of an autochthonous microbial consortium isolated from long-term PAH-contaminated soil has the potential to enhance the bioremediation process.

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

The aim of this study was to isolate microorganisms with bioremediation potential from soil contaminated with heavy metal compounds, and to analyze the tolerance of these microorganisms to various heavy metal concentrations. In total, 7 bacterial strains were isolated and cultivated. The sensitivity of microorganisms to heavy metals proved to be dependent on the type of medium. The use of liquid media during the 8-hour and 24-hour toxicity tests resulted in reduced tolerance of isolates to the concentrations of selective factors used, relative to culture on solid substrates. In addition, optical density increases in the bacterial cultures have been observed at the highest concentrations of some heavy metals. Briefly, during the 48-hours test 3 strains showed increased optical density values in the presence of 1 mM copper sulfate and 2 strains displayed this ability also in the presence of 1 mM cadmium sulfate and lead nitrate.

The pet food industry’s growth, driven by increased demand for premium options, emphasizes the popularity of canned dog foods due to their palatability and convenience. This study evaluates the nutritional and microbiological quality of canned dog food for puppies and adult dogs, with and without grains, immediately after opening and following 24 h of incubation simulating home storage conditions. The grain-free products exhibited higher protein and energy levels, while the grain-included products contained more ether extract, crude ash, and nitrogen-free extract. Age-specific differences revealed higher ether extract, crude ash, crude fiber, and energy in growing dog foods and more nitrogen-free extract in adult dog foods. Discrepancies between labeled nutrient values and laboratory results indicated overstatements for ether extract and underestimations for protein and crude ash. A microbiological analysis showed that the canned dog foods were generally safe when opened, but after 24 h, 85% exhibited bacterial presence, emphasizing the need for rigorous evaluation. A statistical analysis did not confirm associations between grain presence or age group and microbiological contamination. This study underscores the importance of accurate labeling for nutritional value and emphasizes the need for microbiological safety evaluations, especially in regions lacking specific regulatory standards, to ensure pet food safety and nutritional adequacy.

Bovine mastitis is the most common disease affecting dairy cattle worldwide and it generates substantial losses for cattle breeders. One of the most common pathogens identified in infected milk samples is Staphylococcus aureus. Currently, there is no fast test for recognizing bacteria species on the market. The aim of this study was to bioinformatically and laboratory detect and characterize the fibronectin binding protein A (FnBPA) of S. aureus (SA) in milk samples obtained from cows diagnosed with mastitis. More than 90,000,000 amino acid sequences were subjected to bioinformatic detection in the search for a potential biomarker for bovine SA. The analysis of FnBPA included the detection of signal peptides and nonclassical proteins, antigenicity, and the prediction of epitopes. To confirm the presence of the fnbA gene in four SA isolates, amplification with specific primers was performed. FnBPA was detected by immunoblotting. The immunoreactivity and selectivity were performed with monoclonal anti-FnBPA antibodies and SA-negative serum. The bioinformatic analysis showed that FnBPA is a surface, conservative, immunoreactive, and species-specific protein with antigenic potential. Its presence was confirmed in all of the SA isolates we studied. Immunoblotting proved its immunoreactivity and specificity. Thus, it can be considered a potential biomarker in mastitis immunodiagnostics.