The review addresses various aspects of the interaction between carbon nanotubes (CNTs) and microorganisms: the antimicrobial effects of single-walled, multiwalled, functionalized, and...

This review summarizes the information concerning the following applications of microbial biofilms in biotechnology: the biodegradation of organic substances and other contaminants during wastewater treatment, biosynthesis, and biocatalysis. The main types of reactors for implementing biotechnical processes based on microbial biofilms are discussed. The advantages and drawbacks of biocatalysts in the form of microbial biofilms for the biotransformation of organic substances are examined.

The article investigates the expression of oxyR and soxS oxidative stress genes in E. coli under the effect of pristine multi-walled carbon nanotubes (MWCNTs) and pristine single-walled carbon nanotubes (SWCNTs), MWCNTs and SWCNTs functionalized with carboxyl groups (MWCNTs-COOH and SWCNTs-COOH, respectively), SWCNTs functionalized with amino groups (SWCNTs-NH₂) and SWCNTs functionalized with octadecylamine (SWCNTs-ODA). Significant differences were found in the expression of the soxS gene, while no changes were observed in the expression level of the oxyR gene. The pro-oxidant effect of SWCNTs, SWCNTs-COOH, SWCNTs-NH₂, and SWCNTs-ODA is presented, and the contrary antioxidant effect of pristine MWCNTs and MWCNTs-COOH in the presence of methyl viologen hydrate (paraquat) is shown. The article shows that SWCNTs-COOH, SWCNTs-NH₂, and SWCNTs-ODA added to the medium generate the production of reactive oxygen species (ROS) in bacterial cells. SWCNTs-COOH intensified the E. coli biofilm formation, and the biofilm biomass exceeded the control by 25 times. Additionally, it is shown that the rpoS expression increased in response to MWCNTs-COOH and SWCNTs-COOH, and the effect of SWCNTs-COOH was more significant. SWCNTs-COOH and SWCNTs-NH₂ initiated an increase in ATP concentration in the planktonic cells and a decrease in the biofilm cells. The atomic force microscopy (AFM) method showed that the volume of E. coli planktonic cells after the exposure to carbon nanotubes (CNTs) decreased compared to that without exposure, mainly due to a decrease in cell height. The absence of a strong damaging effect of functionalized SWCNTs on E. coli K12 cells, both in suspension and in biofilms, is shown. Contact with functionalized SWCNTs initiated the aggregation of the polymeric substances of the biofilms; however, the cells did not lyse. Among the studied CNTs, SWCNTs-COOH caused an increased expression of the soxS and rpoS, the formation of ROS, and stimulation of the biofilm formation.

POLYMER HYDROGELS IN AGRICULTURE (review) Yu.G. Maksimova, V.A. Shchetko, A.Yu. Maksimov

Releasing multi-walled carbon nanotubes (MWCNTs) into ecosystems affects the biofilm formation and metabolic activity of bacteria in aquatic and soil environments. Pristine (pMWCNTs), oleophilic (oMWCNTs), hydrophilic (hMWCNTs), and carboxylated (cMWCNTs) carbon nanotubes were used to investigate their effects on bacterial biofilm. A pronounced probiofilm effect of modified MWCNTs was observed on the Gram-negative bacteria of Pseudomonas fluorescens C2, Acinetobacter guillouiae 11 h, and Alcaligenes faecalis 2. None of the studied nanomaterials resulted in the complete inhibition of biofilm formation. The complete eradication of biofilms exposed to MWCNTs was not observed. The functionalization of carbon nanotubes was shown to change their probiofilm and antibiofilm effects. Gram-negative bacteria were the most susceptible to destruction, and among the modified MWCNTs, oMWCNTs had the greatest effect on biofilm destruction. The number of living cells in the biofilms was assessed by the reduction of XTT, and metabolic activity was assessed by the reduction of resazurin to fluorescent resorufin. The biofilms formed in the presence of MWCNTs reduced tetrozolium to formazan more actively than the control biofilms. When mature biofilms were exposed to MWCNTs, dehydrogenase activity decreased in Rhodococcus erythropolis 4-1, A. guillouiae 11 h, and A. faecalis 2 in the presence of pMWCNTs and hMWCNTs, as well as in A. guillouiae 11 h exposed to cMWCNTs. When mature biofilms were exposed to pMWCNTs, hMWCNTs, and cMWCNTs, the metabolism of cells decreased in most strains, and oMWCNTs did not have a pronounced inhibitory effect. The antibiofilm and probiofilm effects of MWCNTs were strain-dependent.

The ability of the Alcaligenes faecalis2 strain to utilize acrylamide and acrylic acid upon cultivation with these compounds as the only sources of carbon and energy has been investigated. Complete...