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Dr. Seiya TSUJIMURA
Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba, Japan

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0 Electrochemistry
0 Mediator
0 Bioelectrochemistry
0 biosensor
0 Porous carbon

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Oxidase
Mediator
Dehydrogenase
Porous carbon
Biofuel cell
Direct electron transfer
Bioelectrocatalysis
Electrochemistry
biosensor
electrocatalyst
Carbon cryogel
redox enzyme

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Communication
Published: 16 June 2021 in Chemical Communications
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A bio-conjugated redox network matrix based on glucose dehydrogenase, thionine (diamine-containing mediator), and poly(ethylene glycol) diglycidyl ether (crosslinker) is developed on a glassy carbon electrode through covalent bonding with one-pot crosslinking. Electrons from the enzyme diffuse through the network producing 400 μA cm−2 of glucose oxidation current at 25 °C.

ACS Style

Motaher M. Hossain; Jannatul Morshed; Seiya Tsujimura. Designing a cross-linked redox network for a mediated enzyme-based electrode. Chemical Communications 2021, 1 .

AMA Style

Motaher M. Hossain, Jannatul Morshed, Seiya Tsujimura. Designing a cross-linked redox network for a mediated enzyme-based electrode. Chemical Communications. 2021; ():1.

Chicago/Turabian Style

Motaher M. Hossain; Jannatul Morshed; Seiya Tsujimura. 2021. "Designing a cross-linked redox network for a mediated enzyme-based electrode." Chemical Communications , no. : 1.

Paper
Published: 08 June 2021 in RSC Advances
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Bio-composite inks based on magnesium oxide (MgO)-templated mesoporous carbon (MgOC) and chitosan cross-linked with genipin for one-step screen-printing process.

ACS Style

Isao Shitanda; Kanako Oda; Noya Loew; Hikari Watanabe; Masayuki Itagaki; Seiya Tsujimura; Abdelkader Zebda. Chitosan-based enzyme ink for screen-printed bioanodes. RSC Advances 2021, 11, 20550 -20556.

AMA Style

Isao Shitanda, Kanako Oda, Noya Loew, Hikari Watanabe, Masayuki Itagaki, Seiya Tsujimura, Abdelkader Zebda. Chitosan-based enzyme ink for screen-printed bioanodes. RSC Advances. 2021; 11 (33):20550-20556.

Chicago/Turabian Style

Isao Shitanda; Kanako Oda; Noya Loew; Hikari Watanabe; Masayuki Itagaki; Seiya Tsujimura; Abdelkader Zebda. 2021. "Chitosan-based enzyme ink for screen-printed bioanodes." RSC Advances 11, no. 33: 20550-20556.

Journal article
Published: 21 May 2021 in Biosensors and Bioelectronics
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Glucose level measurement is essential for the point-of-care diagnosis, primarily for persons with diabetes. A disposable electrochemical glucose sensor is constructed using flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) and redox mediator for electron transfer from the enzyme to the electrode surface. Ideally, a suitable mediator should have high water solubility, high kinetic constant, high stability, and redox potential between −0.2 and 0.1 V vs. Ag|AgCl|sat. KCl. We designed and synthesized two new quinone-based water-soluble mediators: quinoline-5,8-dione (QD) and isoquinoline-5,8-dione (IQD). The formal potentials for both QD and IQD at pH 7.0 were −0.07 V vs. Ag|AgCl|sat. KCl. The logarithms of the electron exchange rate constants (k2/(M−1 s−1)) between QD/IQD and FAD-GDH were 7.7 ± 0.1 and 7.4 ± 0.1 for QD and IQD, respectively, which are the highest value among the water-soluble mediators for FAD-GDH reported to date. Disposable amperometric glucose sensors were fabricated by dropping FAD-GDH and QD or IQD onto a test strip. The sensor achieved a linear response up to glucose concentrations of 55.5 mM. The linear response was obtained even when the mediator loading was low (0.5 nmol/strip); loading was only 0.2 mol% of glucose. The results proved that the response current was primarily controlled by glucose diffusion. In addition, the sensor using QD exhibited high stability over 3 months at room temperature.

ACS Style

Jannatul Morshed; Ryo Nakagawa; Motaher M. Hossain; Yuta Nishina; Seiya Tsujimura. Disposable electrochemical glucose sensor based on water-soluble quinone-based mediators with flavin adenine dinucleotide-dependent glucose dehydrogenase. Biosensors and Bioelectronics 2021, 189, 113357 .

AMA Style

Jannatul Morshed, Ryo Nakagawa, Motaher M. Hossain, Yuta Nishina, Seiya Tsujimura. Disposable electrochemical glucose sensor based on water-soluble quinone-based mediators with flavin adenine dinucleotide-dependent glucose dehydrogenase. Biosensors and Bioelectronics. 2021; 189 ():113357.

Chicago/Turabian Style

Jannatul Morshed; Ryo Nakagawa; Motaher M. Hossain; Yuta Nishina; Seiya Tsujimura. 2021. "Disposable electrochemical glucose sensor based on water-soluble quinone-based mediators with flavin adenine dinucleotide-dependent glucose dehydrogenase." Biosensors and Bioelectronics 189, no. : 113357.

Journal article
Published: 05 May 2021 in Electrochemistry
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ACS Style

Yuto Nakagawa; Seiya Tsujimura. Fabrication of an Organic Redox Capacitor with a Neutral Aqueous Electrolyte Solution. Electrochemistry 2021, 89, 317 -322.

AMA Style

Yuto Nakagawa, Seiya Tsujimura. Fabrication of an Organic Redox Capacitor with a Neutral Aqueous Electrolyte Solution. Electrochemistry. 2021; 89 (3):317-322.

Chicago/Turabian Style

Yuto Nakagawa; Seiya Tsujimura. 2021. "Fabrication of an Organic Redox Capacitor with a Neutral Aqueous Electrolyte Solution." Electrochemistry 89, no. 3: 317-322.

Accepted manuscript
Published: 03 March 2021 in Journal of Physics: Energy
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This topical review focuses on self-powered electrochemical sensor devices that use wearable biofuel cells (BFCs) which generate electricity from body fluid components, such as glucose in urine and lactate in sweat. The advantages of using BFCs as power sources for wearable health monitoring devices are discussed herein. Recently developed porous carbon materials with controlled interfaces and spaces are also explored for enhancing the output power and stability of BFCs. We describe a printed wearable high power BFC that uses body fluids as a fuel. This topical review also explains several challenges existing in the development of self-driving health monitoring devices, such as their power output, and stability.

ACS Style

Isao Shitanda; Seiya Tsujimura. Toward self-powered real-time health monitoring of body fluid components based on improved enzymatic biofuel cells. Journal of Physics: Energy 2021, 3, 032002 .

AMA Style

Isao Shitanda, Seiya Tsujimura. Toward self-powered real-time health monitoring of body fluid components based on improved enzymatic biofuel cells. Journal of Physics: Energy. 2021; 3 (3):032002.

Chicago/Turabian Style

Isao Shitanda; Seiya Tsujimura. 2021. "Toward self-powered real-time health monitoring of body fluid components based on improved enzymatic biofuel cells." Journal of Physics: Energy 3, no. 3: 032002.

Journal article
Published: 30 January 2021 in Journal of Power Sources
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Printable wearable lactate biosensors have attracted significant attention, and printable lactate biofuel cells have gained popularity as suitable power supplies for these sensors. However, realizing an appropriate power supply for the practical application of these sensors as wearable devices, it is necessary to improve the output of lactate biofuel cells. In this study, we fabricated a lactate biofuel cell that employed paper substrate using screen printing. The proposed paper-based biofuel cell (PBFC) features a novel electrode design that affords an open circuit voltage of approximately 3.4 V when using an array with six cells in series. Furthermore, a 6 × 6 array of the lactate biofuel cell, i.e., an array comprising six cells in series and six cells in parallel, yielded a power output of 4.3 mW. To the best of our knowledge, this output of the proposed design is higher than those of previously reported lactate biofuel cells. Arrays of the proposed cell were capable of driving a Bluetooth low-energy device for wireless transmission, without requiring a booster circuit. A commercially available activity meter could be driven for 1.5 h using artificial sweat to fuel a 6 × 6 array of the PBFCs.

ACS Style

Isao Shitanda; Yukiya Morigayama; Risa Iwashita; Himeka Goto; Tatsuo Aikawa; Tsutomu Mikawa; Yoshinao Hoshi; Masayuki Itagaki; Hiroyuki Matsui; Shizuo Tokito; Seiya Tsujimura. Paper-based lactate biofuel cell array with high power output. Journal of Power Sources 2021, 489, 229533 .

AMA Style

Isao Shitanda, Yukiya Morigayama, Risa Iwashita, Himeka Goto, Tatsuo Aikawa, Tsutomu Mikawa, Yoshinao Hoshi, Masayuki Itagaki, Hiroyuki Matsui, Shizuo Tokito, Seiya Tsujimura. Paper-based lactate biofuel cell array with high power output. Journal of Power Sources. 2021; 489 ():229533.

Chicago/Turabian Style

Isao Shitanda; Yukiya Morigayama; Risa Iwashita; Himeka Goto; Tatsuo Aikawa; Tsutomu Mikawa; Yoshinao Hoshi; Masayuki Itagaki; Hiroyuki Matsui; Shizuo Tokito; Seiya Tsujimura. 2021. "Paper-based lactate biofuel cell array with high power output." Journal of Power Sources 489, no. : 229533.

Accepted manuscript
Published: 05 January 2021 in Journal of Physics: Energy
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Herein, we describe the effect of varying anions in an electrolyte solution on current generation by a redox hydrogel electrode. The electrode surface is coated with a thin film of hydrogel matrix, consisting of an Os redox polymer including tethered osmium (Os) complexes, polymer backbone, and a redox enzyme. In this case the enzymes employed are flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), which catalyzes glucose oxidation, and the result was compared with that reported earlier for glucose oxidase (GOx). The hydrogel matrix facilitates efficient electron transfer from glucose to the electrode via collision of the Os complexes and thus acts as a mediator. The degree of impact of anions on current generation is characteristic of the Hofmeister series. Chaotropic anions, such as nitrate and chloride, increase and decrease the catalytic current produced by FAD-GDH and GOx hydrogel electrodes, respectively. Such anions can adsorb onto the cationic region of FAD-GDH surface and induce a negative charge, which enhances electrostatic interactions between the enzyme and the positively charged Os polymer. Kosmotropic anions, such as sulphate and phosphate increase catalytic current due to hydrogel shrinkage, which increases the relative concentrations of both enzyme and mediator within the hydrogel architecture due to an increase in density. High-performance electrode design depends on understanding the impact of ion identity on catalytic current responses of redox hydrogel electrodes.

ACS Style

Aimi Yoshida; Seiya Tsujimura. Improved glucose oxidation catalytic current generation by an FAD-dependent glucose dehydrogenase-modified hydrogel electrode, in accordance with the Hofmeister effect. Journal of Physics: Energy 2021, 3, 024005 .

AMA Style

Aimi Yoshida, Seiya Tsujimura. Improved glucose oxidation catalytic current generation by an FAD-dependent glucose dehydrogenase-modified hydrogel electrode, in accordance with the Hofmeister effect. Journal of Physics: Energy. 2021; 3 (2):024005.

Chicago/Turabian Style

Aimi Yoshida; Seiya Tsujimura. 2021. "Improved glucose oxidation catalytic current generation by an FAD-dependent glucose dehydrogenase-modified hydrogel electrode, in accordance with the Hofmeister effect." Journal of Physics: Energy 3, no. 2: 024005.

Accepted manuscript
Published: 14 December 2020 in Journal of Physics: Energy
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Biofuel cell which can generate electricity with only water is expected to use as a new energy harvester for an emergency power supply. A new 4-series/4-parallel structured paper-substrate biofuel cell was prepared using a fuel supply paper preloaded with glucose and phosphate buffer salts. When a power generation test was conducted by supplying water to the fuel-preloaded paper, the paper-based biofuel cell produced an output approximately 90% (0.84 mW) of that obtained by supplying a phosphate buffer containing glucose as the electrolyte. The open-circuit voltage was 2.1 V, and an LED could be powered by simply supplying water to the cell without using a booster circuit.

ACS Style

Isao Shitanda; Misaki Momiyama; Yoshinao Hoshi; Masayuki Itagaki; Seiya Tsujimura. Ready-to-use paper biofuel cell driven by water. Journal of Physics: Energy 2020, 3, 016001 .

AMA Style

Isao Shitanda, Misaki Momiyama, Yoshinao Hoshi, Masayuki Itagaki, Seiya Tsujimura. Ready-to-use paper biofuel cell driven by water. Journal of Physics: Energy. 2020; 3 (1):016001.

Chicago/Turabian Style

Isao Shitanda; Misaki Momiyama; Yoshinao Hoshi; Masayuki Itagaki; Seiya Tsujimura. 2020. "Ready-to-use paper biofuel cell driven by water." Journal of Physics: Energy 3, no. 1: 016001.

Accepted manuscript
Published: 10 December 2020 in Journal of Physics: Energy
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Electrochemical grafting is a suitable technology for fabricating electrode surfaces with new chemical functionalities whilst maintaining the bulk properties of the electrode, and electrochemical amine oxidation and diazonium salt reduction are two widely used techniques to achieve this end. Herein, we report the electrochemical reductive grafting of Azure A onto multiwalled carbon nanotube (MWCNT) electrodes for the efficient wiring of flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (FADGDH). The diazonium salt of Azure A is formed in situ and subsequently grafted onto the electrode surface through electrochemical reduction. The formal potential of the resultant Azure-A-modified electrode shifted to -0.05 V vs. Ag/AgCl upon radical coupling to the MWCNT electrode. Electron transfer from FAD buried in the protein shell to the electrode via Azure A was then observed in the presence of glucose in the buffer solution. This study focused on the important effect of CNT mass loading on Azure-A loading as well as bioelectrocatalytic activity and storage stability. The three-dimensional porous structure of the MWCNT electrode was determined to be favorable for the immobilization of FADGDH and efficient electron transfer via the Azure-A functionalities. The optimized 300-µg CNT-loaded modified electrode on glassy carbon (3-mm diameter) retains its initial activity for 3 d and 25% of its initial activity after 10 d. Furthermore, we show that grafted Azure A is stably immobilized on the MWCNTs for one month; therefore, the limiting stability factor is enzyme leaching and/or deactivation.

ACS Style

Seiya Tsujimura; Shunya Tanaka; Andrew Gross; Michael Holzinger. Electrochemical modification at multiwalled carbon nanotube electrodes with Azure A for FAD- glucose dehydrogenase wiring: structural optimization to enhance catalytic activity and stability. Journal of Physics: Energy 2020, 3, 024004 .

AMA Style

Seiya Tsujimura, Shunya Tanaka, Andrew Gross, Michael Holzinger. Electrochemical modification at multiwalled carbon nanotube electrodes with Azure A for FAD- glucose dehydrogenase wiring: structural optimization to enhance catalytic activity and stability. Journal of Physics: Energy. 2020; 3 (2):024004.

Chicago/Turabian Style

Seiya Tsujimura; Shunya Tanaka; Andrew Gross; Michael Holzinger. 2020. "Electrochemical modification at multiwalled carbon nanotube electrodes with Azure A for FAD- glucose dehydrogenase wiring: structural optimization to enhance catalytic activity and stability." Journal of Physics: Energy 3, no. 2: 024004.

Journal article
Published: 27 August 2020 in Journal of Power Sources
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We have developed a new electrode material for biofuel cells (BFCs) in which a mediator was covalently immobilized on MgO-templated porous carbon (MgOC). Grafted MgOC (GMgOC) was prepared by modifying poly (glycidyl methacrylate) on the MgOC surface by electron beam graft polymerization. Furthermore, aminoferrocene (AmFc) and flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) were immobilized by covalent bonding between the amino group and pendant glycidyl group. The current density estimated by cyclic voltammetry was approximately 21.2 mA cm−2 at 0.49 V in a 1 mol L−1 phosphate buffer containing 100 mmol L−1 glucose. The current value was stable because of the suppression of AmFc elution after 10 h of continuous measurement. A glucose/O2 BFC was fabricated by combining an anode modified with FAD-GDH/poly(GMA)-modified AmFc and a BOD/2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-modified water-repellent cathode and applying 1 mol L−1 PBS containing 300 mmol L−1 glucose (pH 7.0). The BFC showed a high power density of 3.6 mW cm−2. We fabricated a wearable BFC based on hydrogels with ortho hydroquinone groups that can maintain adhesiveness on human skin. The power density of the wearable cell was 0.42 mW cm−2 at 0.38 V in the air, which is sufficient output to power a small electronic device.

ACS Style

Ryo Suzuki; Isao Shitanda; Tatsuo Aikawa; Toshifumi Tojo; Takeshi Kondo; Seiya Tsujimura; Masayuki Itagaki; Makoto Yuasa. Wearable glucose/oxygen biofuel cell fabricated using modified aminoferrocene and flavin adenine dinucleotide-dependent glucose dehydrogenase on poly(glycidyl methacrylate)-grafted MgO-templated carbon. Journal of Power Sources 2020, 479, 228807 .

AMA Style

Ryo Suzuki, Isao Shitanda, Tatsuo Aikawa, Toshifumi Tojo, Takeshi Kondo, Seiya Tsujimura, Masayuki Itagaki, Makoto Yuasa. Wearable glucose/oxygen biofuel cell fabricated using modified aminoferrocene and flavin adenine dinucleotide-dependent glucose dehydrogenase on poly(glycidyl methacrylate)-grafted MgO-templated carbon. Journal of Power Sources. 2020; 479 ():228807.

Chicago/Turabian Style

Ryo Suzuki; Isao Shitanda; Tatsuo Aikawa; Toshifumi Tojo; Takeshi Kondo; Seiya Tsujimura; Masayuki Itagaki; Makoto Yuasa. 2020. "Wearable glucose/oxygen biofuel cell fabricated using modified aminoferrocene and flavin adenine dinucleotide-dependent glucose dehydrogenase on poly(glycidyl methacrylate)-grafted MgO-templated carbon." Journal of Power Sources 479, no. : 228807.

Journal article
Published: 21 April 2020 in Colloids and Surfaces B: Biointerfaces
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Electrochemically polymerized phenothiazines (thionine, methylene green, methylene blue, and toluidine blue) on carbon electrodes were investigated as electron transfer mediators of glucose oxidation by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) for biosensor and biofuel cell applications. Among the tested polyphenothiazines grafted on a glassy carbon electrode, clear redox-mediating activity was observed for poly(methylene green), and the catalytic oxidation current depended on the concentrations of glucose and enzymes and the amount of polymer deposited on the electrode surface. The poly(methylene green)-grafted porous carbon electrodes showed 3 mA cm−2 of glucose oxidation current catalyzed by FAD-GDH.

ACS Style

Nozomu Tsuruoka; Silvia Sato Soto; Awatef Ben Tahar; Abdelkader Zebda; Seiya Tsujimura. Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase. Colloids and Surfaces B: Biointerfaces 2020, 192, 111065 .

AMA Style

Nozomu Tsuruoka, Silvia Sato Soto, Awatef Ben Tahar, Abdelkader Zebda, Seiya Tsujimura. Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase. Colloids and Surfaces B: Biointerfaces. 2020; 192 ():111065.

Chicago/Turabian Style

Nozomu Tsuruoka; Silvia Sato Soto; Awatef Ben Tahar; Abdelkader Zebda; Seiya Tsujimura. 2020. "Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase." Colloids and Surfaces B: Biointerfaces 192, no. : 111065.

Journal article
Published: 20 March 2020 in Electrochimica Acta
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For the efficient electrochemical glucose oxidation on the electrode modified with redox hydrogel involving Os-tethered polymer and flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH), the effect of pore size of MgO-templated carbon (MgOC) as an electrode material was studied. The MgOC was modified on glassy carbon electrode by ink-drop-casting technique. The MgOC pore size clearly affected on the current generation efficiency for glucose oxidation. As the pore size increased above 100 nm, a glucose oxidation current density of more than 100 mA cm−2 was achieved with 1000 μg cm−2 of hydrogel loading. Both high specific surface area and macrostructure of MgOC, which does not impede mass transport even if hydrogel loading is increased, are important factors in designing the porous structure of the MgOC layer via the ink-drop-casting process during electrode fabrication.

ACS Style

Seiya Tsujimura; Satoshi Takeuchi. Toward an ideal platform structure based on MgO-templated carbon for flavin adenine dinucleotide-dependent glucose dehydrogenase-Os polymer-hydrogel electrodes. Electrochimica Acta 2020, 343, 136110 .

AMA Style

Seiya Tsujimura, Satoshi Takeuchi. Toward an ideal platform structure based on MgO-templated carbon for flavin adenine dinucleotide-dependent glucose dehydrogenase-Os polymer-hydrogel electrodes. Electrochimica Acta. 2020; 343 ():136110.

Chicago/Turabian Style

Seiya Tsujimura; Satoshi Takeuchi. 2020. "Toward an ideal platform structure based on MgO-templated carbon for flavin adenine dinucleotide-dependent glucose dehydrogenase-Os polymer-hydrogel electrodes." Electrochimica Acta 343, no. : 136110.

Journal article
Published: 03 October 2019 in Sustainability
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Nutrient recovery from source-separated human urine has been identified by many as a viable avenue towards the circular economy of nutrients. Moreover, untreated (and partially treated) urine is the main anthropogenic route of environmental discharge of nutrients, most concerning for nitrogen, whose release has exceeded the planet’s own self-healing capacity. Urine contains all key macronutrients (N, P, and K) and micronutrients (S, Ca, Mg, and trace metals) needed for plant growth and is, therefore, an excellent fertilizer. However, direct reuse is not recommended in modern society due to the presence of active organic molecules and heavy metals in urine. Many systems have been proposed and tested for nutrient recovery from urine, but none so far has reached technological maturity due to usually high power or chemical requirements or the need for advanced process controls. This work is the proof of concept for the world’s first nutrient recovery system that powers itself and does not require any chemicals or process controls. This is a variation of the previously proposed microbial electrochemical Ugold process, where a novel air cathode catalyst active in urine conditions (pH 9, high ammonia) enables in situ generation of electricity in a microbial fuel cell setup, and the simultaneous harvesting of such electricity for the electrodialytic concentration of ionic nutrients into a product stream, which is free of heavy metals. The system was able to sustain electrical current densities around 3 A m–2 for over two months while simultaneously upconcentrating N and K by a factor of 1.5–1.7.

ACS Style

Stefano Freguia; Maddalena Logrieco; Juliette Monetti; Pablo Ledezma; Bernardino Virdis; Seiya Tsujimura. Self-Powered Bioelectrochemical Nutrient Recovery for Fertilizer Generation from Human Urine. Sustainability 2019, 11, 5490 .

AMA Style

Stefano Freguia, Maddalena Logrieco, Juliette Monetti, Pablo Ledezma, Bernardino Virdis, Seiya Tsujimura. Self-Powered Bioelectrochemical Nutrient Recovery for Fertilizer Generation from Human Urine. Sustainability. 2019; 11 (19):5490.

Chicago/Turabian Style

Stefano Freguia; Maddalena Logrieco; Juliette Monetti; Pablo Ledezma; Bernardino Virdis; Seiya Tsujimura. 2019. "Self-Powered Bioelectrochemical Nutrient Recovery for Fertilizer Generation from Human Urine." Sustainability 11, no. 19: 5490.

Journal article
Published: 20 August 2019 in Electrochimica Acta
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Enzymatic biofuel cells (BFCs) directly convert the chemical energy produced by oxidizing fuel into electricity using enzymes as electrocatalysts. However, these cells are characterized by low output and poor long-term durability, which highlights the need for further performance improvement, especially on the cathode side. Herein, we clarify the effect of the pore size and surface morphology of MgO-templated carbon (MgOC) as an electrode material on the amount of electrochemically active bilirubin oxidase (BOD) available on the cathode. This enzyme is widely used as an electrocatalyst for the four-electron cathodic reduction of oxygen. The amount of electrochemically active enzyme can be increased by using an electrode of larger pore size and by increasing the incubation time for enzyme adsorption. However, excess enzyme prevents the mass transfer of O2 to the enzyme adsorbed on the electrode surface. The MgOC surface was electrochemically modified using several substituted aromatic amines to enhance the interaction between the active sites on BOD and the electrode. Our results showed that the use of 6-amino-2-naphthoic acid as the promoter increased the interfacial electron transfer rate between BOD and the carbon surface and enhanced the stability.

ACS Style

Seiya Tsujimura; Makiko Oyama; Hiroto Funabashi; Shuji Ishii. Effects of pore size and surface properties of MgO-templated carbon on the performance of bilirubin oxidase–modified oxygen reduction reaction cathode. Electrochimica Acta 2019, 322, 134744 .

AMA Style

Seiya Tsujimura, Makiko Oyama, Hiroto Funabashi, Shuji Ishii. Effects of pore size and surface properties of MgO-templated carbon on the performance of bilirubin oxidase–modified oxygen reduction reaction cathode. Electrochimica Acta. 2019; 322 ():134744.

Chicago/Turabian Style

Seiya Tsujimura; Makiko Oyama; Hiroto Funabashi; Shuji Ishii. 2019. "Effects of pore size and surface properties of MgO-templated carbon on the performance of bilirubin oxidase–modified oxygen reduction reaction cathode." Electrochimica Acta 322, no. : 134744.

Short communication
Published: 09 July 2019 in Journal of Power Sources
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As lactate is a useful biomarker for evaluating conditions of the body and also has a high theoretical energy density as a fuel, enzymatic biofuel cells (BFCs) that utilize lactate as a fuel have received considerable attention. In this study, we have developed a high-power lactate/O2 BFC that comprises composite electrodes made from carbon cloth as a current collector and MgO-templated porous carbon as an electroenzymatic reaction field and enzyme support. The bioanode contains lactate oxidase as an enzyme and 1-methoxy-5-methylphenazinium methylsulfate as a redox mediator. The biocathode contains bilirubin oxidase as an oxygen reduction electrocatalyst and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as a mediator. To obtain an efficient gas diffusion biocathode, the hydrophobicity of the carbon cloth is modulated by modification with PTFE. The lactate/O2 BFC shows a maximum power density of 4.3 mW cm−2 at a lactate concentration of 300 mM. This high performance is promising for the development of BFCs for wearable applications.

ACS Style

Isao Shitanda; Kotaro Takamatsu; Ayumu Niiyama; Tsutomu Mikawa; Yoshinao Hoshi; Masayuki Itagaki; Seiya Tsujimura. High-power lactate/O2 enzymatic biofuel cell based on carbon cloth electrodes modified with MgO-templated carbon. Journal of Power Sources 2019, 436, 226844 .

AMA Style

Isao Shitanda, Kotaro Takamatsu, Ayumu Niiyama, Tsutomu Mikawa, Yoshinao Hoshi, Masayuki Itagaki, Seiya Tsujimura. High-power lactate/O2 enzymatic biofuel cell based on carbon cloth electrodes modified with MgO-templated carbon. Journal of Power Sources. 2019; 436 ():226844.

Chicago/Turabian Style

Isao Shitanda; Kotaro Takamatsu; Ayumu Niiyama; Tsutomu Mikawa; Yoshinao Hoshi; Masayuki Itagaki; Seiya Tsujimura. 2019. "High-power lactate/O2 enzymatic biofuel cell based on carbon cloth electrodes modified with MgO-templated carbon." Journal of Power Sources 436, no. : 226844.

Journal article
Published: 30 April 2019 in Electrochimica Acta
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Flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) become a key enzyme for electrochemical oxidation of glucose. Here, we investigated the effects of electrolyte, especially at high concentration, on the bioelectrocatalytic activity of FAD-GDH from Aspergillus terreus using a ferricyanide as a redox mediator. In the low ionic strength region, an increase of bimolecular reaction rate constant (k2) between FAD-GDH and ferricyanide was observed due to the reduction of the ionic repulsive interaction between negatively charged enzyme and mediator. Above 0.5 M concentration, ion-specific increments in k2 were observed; ammonium ions most efficiently increased k2, owing to adsorption via dispersion force on the enzyme surface likely proximal to the active site.

ACS Style

Tsubasa Adachi; Seiya Tsujimura. Effects of electrolyte on the mediated electrocatalytic glucose oxidation reaction catalyzed by flavin adenine dinucleotide glucose dehydrogenase. Electrochimica Acta 2019, 313, 189 -193.

AMA Style

Tsubasa Adachi, Seiya Tsujimura. Effects of electrolyte on the mediated electrocatalytic glucose oxidation reaction catalyzed by flavin adenine dinucleotide glucose dehydrogenase. Electrochimica Acta. 2019; 313 ():189-193.

Chicago/Turabian Style

Tsubasa Adachi; Seiya Tsujimura. 2019. "Effects of electrolyte on the mediated electrocatalytic glucose oxidation reaction catalyzed by flavin adenine dinucleotide glucose dehydrogenase." Electrochimica Acta 313, no. : 189-193.

Journal article
Published: 20 April 2019 in Journal of Power Sources
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We report enzymatic biofuel cells (EBFCs) based on MgO-templated carbon (MgOC)-carbon textile composite electrodes, which are lightweight, flexible, and used as liquid containers. MgOC particles with a pore size of 40 nm were modified on a carbon cloth substrate using poly(vinylidenedifluoride) as an anodic binder and polytetrafluoroethylene as a cathodic binder. Flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) from Aspergillus terreus was used for the anode, with 1,4-naphthoquinone chosen as the redox mediator. The O2-reducing cathode contains bilirubin oxidase (BOD) from Myrocecium verucaria and 2,2′-azinobis(3-ethylbenzothiazolin-6-sulfonate) (ABTS), which facilitates electron transfer between the active site of BOD and the electrode. The hydrophobicity of the gas diffusion layer in the textile composite cathode is tuned with the aid of a PTFE binder, to increase the amount of O2 supplied from the air. The open circuit potential is 0.75 V and maximum output power density is 2 mW cm−2 at 0.4 V (room temperature, without O2 gas flow). Our results clearly indicate that MgOC-textile materials are very promising for the development of high-performance, wearable EBFCs.

ACS Style

Ayumu Niiyama; Kazuki Murata; Yasushi Shigemori; Abdelkader Zebda; Seiya Tsujimura. High-performance enzymatic biofuel cell based on flexible carbon cloth modified with MgO-templated porous carbon. Journal of Power Sources 2019, 427, 49 -55.

AMA Style

Ayumu Niiyama, Kazuki Murata, Yasushi Shigemori, Abdelkader Zebda, Seiya Tsujimura. High-performance enzymatic biofuel cell based on flexible carbon cloth modified with MgO-templated porous carbon. Journal of Power Sources. 2019; 427 ():49-55.

Chicago/Turabian Style

Ayumu Niiyama; Kazuki Murata; Yasushi Shigemori; Abdelkader Zebda; Seiya Tsujimura. 2019. "High-performance enzymatic biofuel cell based on flexible carbon cloth modified with MgO-templated porous carbon." Journal of Power Sources 427, no. : 49-55.

Communication
Published: 13 November 2018 in ChemElectroChem
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The thermal stability of flavin adenine dinucleotide‐dependent glucose dehydrogenase (FAD‐GDH) from Aspergillus terreus in phosphate buffer solution was substantially improved by kosmotropic ions, especially kosmotropic anions. The residual activity of FAD‐GDH in a 1.5 M sodium or ammonium sulfate solution remained more than 90 % after 60 min of heat treatment at 60 °C, while slight activity was observed for FAD‐GDH in a solution in the absence of additive sulfate ions. The stabilizing effect of the electrolytes was concentration‐dependent and strongly related to the structural stabilization of the enzyme, which involved enzyme compaction.

ACS Style

Miki Nemoto; Kazunori Sugihara; Tsubasa Adachi; Kazuki Murata; Kentaro Shiraki; Seiya Tsujimura. Effect of Electrolyte Ions on the Stability of Flavin Adenine Dinucleotide‐Dependent Glucose Dehydrogenase. ChemElectroChem 2018, 6, 1028 -1031.

AMA Style

Miki Nemoto, Kazunori Sugihara, Tsubasa Adachi, Kazuki Murata, Kentaro Shiraki, Seiya Tsujimura. Effect of Electrolyte Ions on the Stability of Flavin Adenine Dinucleotide‐Dependent Glucose Dehydrogenase. ChemElectroChem. 2018; 6 (4):1028-1031.

Chicago/Turabian Style

Miki Nemoto; Kazunori Sugihara; Tsubasa Adachi; Kazuki Murata; Kentaro Shiraki; Seiya Tsujimura. 2018. "Effect of Electrolyte Ions on the Stability of Flavin Adenine Dinucleotide‐Dependent Glucose Dehydrogenase." ChemElectroChem 6, no. 4: 1028-1031.

Journal article
Published: 05 November 2018 in Chemistry Letters
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ACS Style

Daisuke Takimoto; Seiya Tsujimura. Improved Formation of Pt Multilayers at Near-neutral pH: Underpotential Deposition and Surface Limited Redox Replacement. Chemistry Letters 2018, 47, 1379 -1382.

AMA Style

Daisuke Takimoto, Seiya Tsujimura. Improved Formation of Pt Multilayers at Near-neutral pH: Underpotential Deposition and Surface Limited Redox Replacement. Chemistry Letters. 2018; 47 (11):1379-1382.

Chicago/Turabian Style

Daisuke Takimoto; Seiya Tsujimura. 2018. "Improved Formation of Pt Multilayers at Near-neutral pH: Underpotential Deposition and Surface Limited Redox Replacement." Chemistry Letters 47, no. 11: 1379-1382.

Rapid communication
Published: 05 October 2018 in Chemistry Letters
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A technique for immobilizing enzyme on Au electrode was developed. The oxygen reduction reaction activity of electrochemically deposited enzymes was higher than that of physically adsorbed enzymes. Based on the electrochemical quartz crystal microbalance measurements, the higher ORR activity of electrochemically deposited enzymes could be attributed to the proper orientation of the enzymes. In addition, the electrochemically deposited enzymes exhibited high stability with respect to potential cycles.

ACS Style

Daisuke Takimoto; Seiya Tsujimura. Oxygen Reduction Reaction Activity and Stability of Electrochemically Deposited Bilirubin Oxidase. Chemistry Letters 2018, 47, 1269 -1271.

AMA Style

Daisuke Takimoto, Seiya Tsujimura. Oxygen Reduction Reaction Activity and Stability of Electrochemically Deposited Bilirubin Oxidase. Chemistry Letters. 2018; 47 (10):1269-1271.

Chicago/Turabian Style

Daisuke Takimoto; Seiya Tsujimura. 2018. "Oxygen Reduction Reaction Activity and Stability of Electrochemically Deposited Bilirubin Oxidase." Chemistry Letters 47, no. 10: 1269-1271.