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Prof. Shaohua Zeng
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, Guangdong, China

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0 Salinity
0 Transcriptional Regulation
0 anthocyanin
0 epigenetic regulation
0 Carotenoid

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anthocyanin
Carotenoid
fruit ripening
Fruit pigmentation

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Original article
Published: 09 August 2021 in 3 Biotech
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Gene expression valuated by reverse transcription-quantitative PCR (RT-qPCR) are often applied to study the gene function. To obtain accurate and reliable results, the usage of stable reference genes is essential for RT-qPCR analysis. The traditional southern Chinese medicinal herb, Desmodium styracifolium Merr is well known for its remarkable effect on the treatment of urination disturbance, urolithiasis, edema and jaundice. However, there are no ready-made reference genes identified for D. styracifolium. In this study, 13 novel genes retrieved from transcriptome datasets of four different tissues were reported according to the coefficient of variation (CV) and maximum fold change (MFC) of gene expression. The expression stability of currently used Leguminosae ACT6 was compared to the 13 candidate reference genes in different tissues and 7-day-old seedlings under different experimental conditions, which was evaluated by five statistical algorithms (geNorm/NormFinder/BestKeeper/ΔCT/RefFinder). Our results indicated that the reference gene combinations of PP + UFM1, CCRP4 + BRM and NFD6 + NCLN1 were the most stable reference genes in leaf, stem and root tissues, respectively. The most stable reference gene combination for all tissues was CCRP4 + CUL1. In addition, the most stable reference genes for different experimental conditions were distinct, for instance SMUP1 for MeJA treatment, ERDJ2A + SMUP1 for SA treatment, NCLN1 + ERDJ2A for ABA treatment and SF3B + VAMP721d for salt stress, respectively. Our results lay a foundation for achieving accurate and reliable RT-qPCR results so as to correctly understand the function of genes in D. styracifolium.

ACS Style

Zhiqiang Wang; Fangqin Yu; Dingding Shi; Ying Wang; Feng Xu; Shaohua Zeng. Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr. 3 Biotech 2021, 11, 1 -14.

AMA Style

Zhiqiang Wang, Fangqin Yu, Dingding Shi, Ying Wang, Feng Xu, Shaohua Zeng. Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr. 3 Biotech. 2021; 11 (9):1-14.

Chicago/Turabian Style

Zhiqiang Wang; Fangqin Yu; Dingding Shi; Ying Wang; Feng Xu; Shaohua Zeng. 2021. "Selection and validation of reference genes for RT-qPCR analysis in Desmodium styracifolium Merr." 3 Biotech 11, no. 9: 1-14.

Journal article
Published: 23 April 2021 in International Journal of Molecular Sciences
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Salt stress seriously affects yield and quality of crops. The fruit of Lycium barbarum (LBF) is extensively used as functional food due to its rich nutrient components. It remains unclear how salt stress influences the quality of LBF. In this study, we identified 71 differentially accumulated metabolites (DAMs) and 1396 differentially expressed genes (DEGs) among ripe LBF with and without 300 mM of NaCl treatment. Pearson correlation analysis indicated that the metabolomic changes caused by salt stress were strongly related to oxidoreductases; hydrolases; and modifying enzymes, in particular, acyltransferases, methyltransferases and glycosyltransferases. Further analysis revealed that salt stress facilitated flavonoid glycosylation and carotenoid esterification by boosting the expression of structural genes in the biosynthetic pathways. These results suggested that salt stress prompts the modification of flavonoids and carotenoids to alleviate ROS damage, which in turn improves the quality of LBF. Our results lay a solid foundation for uncovering the underlying molecular mechanism of salt stress orchestrating LBF quality, and the candidate genes identified will be a valuable gene resource for genetic improvement of L. barbarum.

ACS Style

Shuang Lin; Shaohua Zeng; Biao A; Xiaoman Yang; Tianshun Yang; Guoqi Zheng; Guilian Mao; Ying Wang. Integrative Analysis of Transcriptome and Metabolome Reveals Salt Stress Orchestrating the Accumulation of Specialized Metabolites in Lycium barbarum L. Fruit. International Journal of Molecular Sciences 2021, 22, 4414 .

AMA Style

Shuang Lin, Shaohua Zeng, Biao A, Xiaoman Yang, Tianshun Yang, Guoqi Zheng, Guilian Mao, Ying Wang. Integrative Analysis of Transcriptome and Metabolome Reveals Salt Stress Orchestrating the Accumulation of Specialized Metabolites in Lycium barbarum L. Fruit. International Journal of Molecular Sciences. 2021; 22 (9):4414.

Chicago/Turabian Style

Shuang Lin; Shaohua Zeng; Biao A; Xiaoman Yang; Tianshun Yang; Guoqi Zheng; Guilian Mao; Ying Wang. 2021. "Integrative Analysis of Transcriptome and Metabolome Reveals Salt Stress Orchestrating the Accumulation of Specialized Metabolites in Lycium barbarum L. Fruit." International Journal of Molecular Sciences 22, no. 9: 4414.

Journal article
Published: 20 March 2020 in Industrial Crops and Products
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The traditional Chinese medicinal plant Desmodium styracifolium Merr. have efficient effect on preventing urinary calculi due to schaftoside. However, the schaftoside biosynthesis remains unknown. In this study, RNAseq transcriptomes and small RNAomes of leaves and flowers were sequenced and analyzed. There are 9955 differentially expressed genes DEGs and 86 differentially expressed microRNA miRNA identified. Pathway enrichment analysis show that phenylpropanoid biosynthesis and phenylalanine metabolism are over-represented in leaves when compared to flowers. A total of 30,934 simple sequence repeat SSR, 17,574 insertion-deletions InDels, and 86,882 single nucleotide polymorphisms SNPs were identified as gene-based markers. Schaftoside biosynthetic genes C-glucosyltransferase CGT and flavone synthase II FNSII and 1484 unigenes coexpressing with CGT and FNSII were predicted. Among them, 1038 unigenes containing gene-based marker coexpressed with CGT and 419 CGT-coexpressing unigenes can be successfully designed SSR markers. Moreover, 107 of 419 CGT-coexpressing unigenes containing SSR were randomly selected to validate the efficiency of SSR primers, which resulting 104 97.2 %) primers successfully amplified the polymerase chain reaction product and 54 (50.5 % primers are polymorphism primers. These results suggested that 211 polymorphic SSR markers could be further used for marker-assisted breeding MAB. Meanwhile, 6436 of 9955 DEGs containing gene-based marker differentially expressed in leaves when compared to flowers. Furthermore, 26 and 184 miRNA-targeted unigenes containing gene-based markers are CGT-coexpressing genes and DEGs, respectively. Candidate genes and gene-based markers identified in this study are useful resource, which lay a solid foundation for studying schaftoside biosynthesis, MAB, genetic map and comparative genomic study of D. styracifolium.

ACS Style

Zhiqiang Wang; Haiguang Gong; Xiaoyong Xu; Xiaoqun Wei; Ying Wang; Shaohua Zeng. Transcriptome and small RNAome facilitate to study schaftoside in Desmodium styracifolium Merr. Industrial Crops and Products 2020, 149, 112352 .

AMA Style

Zhiqiang Wang, Haiguang Gong, Xiaoyong Xu, Xiaoqun Wei, Ying Wang, Shaohua Zeng. Transcriptome and small RNAome facilitate to study schaftoside in Desmodium styracifolium Merr. Industrial Crops and Products. 2020; 149 ():112352.

Chicago/Turabian Style

Zhiqiang Wang; Haiguang Gong; Xiaoyong Xu; Xiaoqun Wei; Ying Wang; Shaohua Zeng. 2020. "Transcriptome and small RNAome facilitate to study schaftoside in Desmodium styracifolium Merr." Industrial Crops and Products 149, no. : 112352.

Journal article
Published: 26 February 2020 in Industrial Crops and Products
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Lycium barbarum L. (LB) and L. ruthenicum Murr. (LR) are the two closet species in Lycium genus phylogenetically. The LB fruit (LBF) contains high level of carotenoids and undetectable anthocyanins, while LR fruit (LRF) has abundant anthocyanins and very low amount of carotenoids. Although the causes of the pigmentation difference in term of gene expression and metabolites were previously investigated in the two species, the underlying mechanism remains unclear at protein level. In this study, the ripening fruits of LB, LR and their hybrid were harvested for comparative proteomic and ultrastructural analysis. Ultrastructural analysis indicates that LBF successfully form abundant tubular chromoplasts, and that LRF fail to develop chromoplasts. This result is confirmed by the expression ratio of rbcLs/PDS as an indicator of plastid number. Proteomic results uncover that the number of photosynthesis-related proteins undetectable in LRF is significant higher than in LBF. Chromoplast-biogenesis protein OR and plastoglobules-localized ABC1K1 is abundant in LBF but undetectable in LRF. Meanwhile, anthocyanin enzyme localizing ER vesiculate seriously in LRF while failed in LBF. The abundance of vacuole-localized anthocyanin trafficking protein SYP22 and vacuolar formation protein VPS4 increased in LRF and decreased/undetectable in LBF. Proteomic results also reveal that differentially expressed proteins related to anthocyanin synthesis and accumulation are enriched and increased in ripening LRF with undetectable certain proteins involved in carotenoid pathway and vice versus for LBF. Altogether, our data provide new insight on ER and vacuole formation and plastid differentiation, which contribute largely to the pigmentation distinct in Lycium fruits.

ACS Style

Shaohua Zeng; Shanshan Huang; Tianshun Yang; Peiyan Ai; Li Li; Ying Wang. Comparative proteomic and ultrastructural analysis shed light on fruit pigmentation distinct in two Lycium species. Industrial Crops and Products 2020, 147, 112267 .

AMA Style

Shaohua Zeng, Shanshan Huang, Tianshun Yang, Peiyan Ai, Li Li, Ying Wang. Comparative proteomic and ultrastructural analysis shed light on fruit pigmentation distinct in two Lycium species. Industrial Crops and Products. 2020; 147 ():112267.

Chicago/Turabian Style

Shaohua Zeng; Shanshan Huang; Tianshun Yang; Peiyan Ai; Li Li; Ying Wang. 2020. "Comparative proteomic and ultrastructural analysis shed light on fruit pigmentation distinct in two Lycium species." Industrial Crops and Products 147, no. : 112267.

Journal article
Published: 09 November 2019 in Food Chemistry
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The Lycium ruthenicum (Lr) fruit is a widely used nutritional food that contains various bioactive components such as anthocyanin and spermidine derivatives. In the present study, ultra-high-performance liquid chromatography with tandem mass spectrometry was utilized to profile the metabolic dynamics of four developmental stages of Lr fruit. A total of 49 compounds, including anthocyanin, alkaloids, hydroxycinnamic acid derivatives, flavonoids, and amino acids, were tentatively identified. Principal component analysis distinguished the fruit at four developmental stages using 15 (9 were tentatively identified) potential marker compounds. Pearson correlation analysis suggested that anthocyanin and spermidine derivative hexoses had a strong positive correlation coefficient. A glucosyltransferase (HG27071) was confirmed to glucosylate both anthocyanidin and spermidine derivative in vitro. Our results provide insight into the metabolic linkages among bioactive components in Lr fruits. The glucosyltransferase identified in this study will promote its potential use in functional foods and natural pigment resources.

ACS Style

Xiaoman Yang; Shuang Lin; Yongxia Jia; Fazal Rehman; Shaohua Zeng; Ying Wang. Anthocyanin and spermidine derivative hexoses coordinately increase in the ripening fruit of Lycium ruthenicum. Food Chemistry 2019, 311, 125874 .

AMA Style

Xiaoman Yang, Shuang Lin, Yongxia Jia, Fazal Rehman, Shaohua Zeng, Ying Wang. Anthocyanin and spermidine derivative hexoses coordinately increase in the ripening fruit of Lycium ruthenicum. Food Chemistry. 2019; 311 ():125874.

Chicago/Turabian Style

Xiaoman Yang; Shuang Lin; Yongxia Jia; Fazal Rehman; Shaohua Zeng; Ying Wang. 2019. "Anthocyanin and spermidine derivative hexoses coordinately increase in the ripening fruit of Lycium ruthenicum." Food Chemistry 311, no. : 125874.

Original article
Published: 03 November 2018 in Plant Cell, Tissue and Organ Culture
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Lycium ruthenicum Murr. is an important medicinal plant from traditional Chinese medicine. It contains various biologically active compounds, such as phenolics and alkaloids. These secondary metabolites are used extensively in dietary food and pharmaceutical products. However, these phenolics occur at very low concentrations in the roots, and thus, it is expensive to commercially produce them. The present study was proposed to induce a hairy root culture system for the first time in L. ruthenicum to achieve a high concentration of phenolic polyamines and other non-targeted secondary metabolites. The over-expression sequence of the TCP4 gene was retrieved from the transcriptome data of L. ruthenicum (LrTCP4-OE), and a gene construct (with pCAMBIA 1307) was integrated into the genome of L. ruthenicum by Ri-mediated genetic transformation. A total of 21 metabolites were tentatively identified by using ultrahigh-performance liquid chromatography coupled to photodiode array detector/quadrupole time-of-flight mass spectrometry (UPLC-PDA-qTOF-MS). Transgenic hairy root clones had higher relative abundances of kukoamine A and 17 other secondary metabolites than did control-type hairy roots. After 1 month, high-growth transgenic and non-transgenic hairy root lines were subjected to UPLC analysis for absolute quantification (with an authentic standard) of total kukoamine A. Transgenic hairy root lines (LrTCP4-OE) showed higher kukoamine A accumulation (0.14%) than did control hairy roots (0.11%). This enhanced productivity correlated with increased TCP4-OE activity, validating the primary role that TCP4 plays in total kukoamine A synthesis and the efficiency of non-targeted metabolomic techniques in studying plant metabolites.

ACS Style

Aysha Arif Chahel; Shaohua Zeng; Zubaida Yousaf; Yinyin Liao; Ziyin Yang; Xiaoyi Wei; Wang Ying. Plant-specific transcription factor LrTCP4 enhances secondary metabolite biosynthesis in Lycium ruthenicum hairy roots. Plant Cell, Tissue and Organ Culture 2018, 136, 323 -337.

AMA Style

Aysha Arif Chahel, Shaohua Zeng, Zubaida Yousaf, Yinyin Liao, Ziyin Yang, Xiaoyi Wei, Wang Ying. Plant-specific transcription factor LrTCP4 enhances secondary metabolite biosynthesis in Lycium ruthenicum hairy roots. Plant Cell, Tissue and Organ Culture. 2018; 136 (2):323-337.

Chicago/Turabian Style

Aysha Arif Chahel; Shaohua Zeng; Zubaida Yousaf; Yinyin Liao; Ziyin Yang; Xiaoyi Wei; Wang Ying. 2018. "Plant-specific transcription factor LrTCP4 enhances secondary metabolite biosynthesis in Lycium ruthenicum hairy roots." Plant Cell, Tissue and Organ Culture 136, no. 2: 323-337.

Original research article
Published: 25 September 2015 in Frontiers in Plant Science
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MicroRNAs (miRNAs) are master regulators of gene activity documented to play central roles in fruit ripening in model plant species, yet little is known of their roles in Lycium barbarum L. fruits. In this study, miRNA levels in L. barbarum fruit samples at four developmental stages, were assayed using Illumina HiSeqTM2000. This revealed the presence of 50 novel miRNAs and 38 known miRNAs in L. barbarum fruits. Of the novel miRNAs, 36 were specific to L. barbarum fruits compared with L. chinense. A number of stage-specific miRNAs were identified and GO terms were assigned to 194 unigenes targeted by miRNAs. The majority of GO terms of unigenes targeted by differentially expressed miRNAs are ‘intracellular organelle’, ‘binding’, ‘metabolic process’, ‘pigmentation’, and ‘biological regulation’. Enriched KEGG analysis indicated that nucleotide excision repair and ubiquitin mediated proteolysis were over-represented during the initial stage of ripening, with ABC transporters and sulfur metabolism pathways active during the middle stages and ABC transporters and spliceosome enriched in the final stages of ripening. Several miRNAs and their targets serving as potential regulators in L. barbarum fruit ripening were identified using quantitative reverse transcription polymerase chain reaction. The miRNA-target interactions were predicted for L. barbarum ripening regulators including miR156/157 with LbCNR and LbWRKY8, and miR171 with LbGRAS. Additionally, regulatory interactions potentially controlling fruit quality and nutritional value via sugar and secondary metabolite accumulation were identified. These include miR156 targeting of fructokinase and 1-deoxy-D-xylulose-5-phosphate synthase and miR164 targeting of beta-fructofuranosidase. In sum, valuable information revealed by small RNA sequencing in this study will provide a solid foundation for uncovering the miRNA-mediated mechanism of fruit ripening and quality in this nutritional food.

ACS Style

Shaohua Zeng; Yongliang Liu; Lizhu Pan; Alice Hayward; Ying Wang. Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L. using high-throughput sequencing. Frontiers in Plant Science 2015, 6, 1 .

AMA Style

Shaohua Zeng, Yongliang Liu, Lizhu Pan, Alice Hayward, Ying Wang. Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L. using high-throughput sequencing. Frontiers in Plant Science. 2015; 6 ():1.

Chicago/Turabian Style

Shaohua Zeng; Yongliang Liu; Lizhu Pan; Alice Hayward; Ying Wang. 2015. "Identification and characterization of miRNAs in ripening fruit of Lycium barbarum L. using high-throughput sequencing." Frontiers in Plant Science 6, no. : 1.

Original research article
Published: 03 September 2015 in Frontiers in Plant Science
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Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. In Epimedium, flavonoids have been demonstrated to be the main bioactive components (BCs). However, the molecular biosynthetic and regulatory mechanisms of flavonoid-derived BCs remain obscure. In this study, we isolated twelve structural genes and two putative transcription factors (TFs) in the flavonoid pathway. Phytochemical analysis showed that the total content of four representative BCs (epimedin A, B, C and icariin) decreased slightly or dramatically in two lines of E. sagittatum during leaf development. Transcriptional analysis revealed that two R2R3-MYB TFs (EsMYBA1 and EsMYBF1), together with a bHLH TF (EsGL3) and WD40 protein (EsTTG1), were supposed to coordinately regulate the anthocyanin and flavonol-derived BCs biosynthesis in leaves. Overexpression of EsFLS (flavonol synthase) in tobacco resulted in increased flavonols content and decreased anthocyanins content in flowers. Moreover, EsMYB12 negatively correlated with the accumulation of the four BCs, and might act as a transcriptional repressor in the flavonoid pathway. Therefore, the anthocyanin pathway may coordinate with the flavonol-derived BCs pathway in Epimedium leaves. A better understanding of the flavonoid biosynthetic and regulatory mechanisms in E. sagittatum will facilitate functional characterization, metabolic engineering and molecular breeding studies of Epimedium species.

ACS Style

Wenjun Huang; Shaohua Zeng; Gong Xiao; Guoyan Wei; Sihong Liao; Jianjun Chen; Wei Sun; Haiyan Lv; Ying Wang. Elucidating the biosynthetic and regulatory mechanisms of flavonoid-derived bioactive components in Epimedium sagittatum. Frontiers in Plant Science 2015, 6, 689 .

AMA Style

Wenjun Huang, Shaohua Zeng, Gong Xiao, Guoyan Wei, Sihong Liao, Jianjun Chen, Wei Sun, Haiyan Lv, Ying Wang. Elucidating the biosynthetic and regulatory mechanisms of flavonoid-derived bioactive components in Epimedium sagittatum. Frontiers in Plant Science. 2015; 6 ():689.

Chicago/Turabian Style

Wenjun Huang; Shaohua Zeng; Gong Xiao; Guoyan Wei; Sihong Liao; Jianjun Chen; Wei Sun; Haiyan Lv; Ying Wang. 2015. "Elucidating the biosynthetic and regulatory mechanisms of flavonoid-derived bioactive components in Epimedium sagittatum." Frontiers in Plant Science 6, no. : 689.

Comparative study
Published: 16 December 2014 in BMC Plant Biology
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The traditional Chinese medicinal plants Lycium barbarum L. and L. ruthenicum Murr. are valued for the abundance of bioactive carotenoids and anthocyanins in their fruits, respectively. However, the cellular and molecular mechanisms contributing to their species-specific bioactive profiles remain poorly understood. In this study, the red fruit (RF) of L. barbarum was found to accumulate high levels of carotenoids (primarily zeaxanthin), while they were undetectable in the black fruit (BF) of L. ruthenicum. Cytological and gene transcriptional analyses revealed that the chromoplast differentiation that occurs in the chloroplast during fruit ripening only occurs in RF, indicating that the lack of chromoplast biogenesis in BF leads to no sink for carotenoid storage and the failure to synthesize carotenoids. Similar enzyme activities of phytoene synthase 1 (PSY1), chromoplast-specific lycopene β-cyclase (CYC-B) and β-carotene hydroxylase 2 (CRTR-B2) were observed in both L. ruthenicum and L. barbarum, suggesting that the undetectable carotenoid levels in BF were not due to the inactivation of carotenoid biosynthetic enzymes. The transcript levels of the carotenoid biosynthetic genes, particularly PSY1, phytoene desaturase (PDS), β-carotene desaturase (ZDS), CYC-B and CRTR-B2, were greatly increased during RF ripening, indicating increased zeaxanthin biosynthesis. Additionally, carotenoid cleavage dioxygenase 4 (CCD4) was expressed at much higher levels in BF than in RF, suggesting continuous carotenoid degradation in BF. The failure of the chromoplast development in BF causes low carotenoid biosynthesis levels and continuous carotenoid degradation, which ultimately leads to undetectable carotenoid levels in ripe BF. In contrast, the successful chromoplast biogenesis in RF furnishes the sink necessary for carotenoid storage. Based on this observation, the abundant zeaxanthin accumulation in RF is primarily determined via both the large carotenoid biosynthesis levels and the lack of carotenoid degradation, which are regulated at the transcriptional level.

ACS Style

Yongliang Liu; Shaohua Zeng; Wei Sun; Min Wu; Weiming Hu; Xiaofei Shen; Ying Wang. Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits. BMC Plant Biology 2014, 14, 269 .

AMA Style

Yongliang Liu, Shaohua Zeng, Wei Sun, Min Wu, Weiming Hu, Xiaofei Shen, Ying Wang. Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits. BMC Plant Biology. 2014; 14 (1):269.

Chicago/Turabian Style

Yongliang Liu; Shaohua Zeng; Wei Sun; Min Wu; Weiming Hu; Xiaofei Shen; Ying Wang. 2014. "Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits." BMC Plant Biology 14, no. 1: 269.

Journal article
Published: 11 September 2013 in Acta Physiologiae Plantarum
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Epimedium is well-known in China and East Asia due to high content of flavonoid derivatives, including icariin, epimedin A, epimedin B, and epimedin C, hereafter designated as bioactive components, which have been extensively utilized to cure many diseases. So far, the molecular mechanism of the bioactive components biosynthesis remains unclear. In the present study, the effect of light stress (24 h illumination) on the accumulation of bioactive components and the expression of flavonoid genes in Epimedium was investigated. Under light stress, the structural genes CHS1, CHI1, F3H, FLS, DFR1, DFR2, and ANS were remarkably up-regulated while CHS2 and F3′H were significantly down-regulated. For transcription factors, the expression of Epimedium MYB7 and TT8 were increased while Epimedium GL3, MYBF, and TTG1 expression were depressed. Additionally, the content of bioactive components was significantly decreased under light stress. Our results suggested that the decrease of bioactive compounds may be attributed to transcripts of late genes (DFRs and ANS) increased to a higher level than that of early genes (FLS and CHS1).

ACS Style

Shaohua Zeng; Yilan Liu; Ying Wang. Light stress suppresses the accumulation of epimedins A, B, C, and icariin in Epimedium, a traditional medicinal plant. Acta Physiologiae Plantarum 2013, 35, 3271 -3275.

AMA Style

Shaohua Zeng, Yilan Liu, Ying Wang. Light stress suppresses the accumulation of epimedins A, B, C, and icariin in Epimedium, a traditional medicinal plant. Acta Physiologiae Plantarum. 2013; 35 (11):3271-3275.

Chicago/Turabian Style

Shaohua Zeng; Yilan Liu; Ying Wang. 2013. "Light stress suppresses the accumulation of epimedins A, B, C, and icariin in Epimedium, a traditional medicinal plant." Acta Physiologiae Plantarum 35, no. 11: 3271-3275.

Journal article
Published: 27 December 2012 in International Journal of Molecular Sciences
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Epimedium sagittatum (Sieb. et Zucc.) Maxim, a popular traditional Chinese medicinal plant, has been widely used for treating sexual dysfunction and osteoporosis in China. The main bioactive components in herba epimedii are prenylated flavonol glycosides, which are end products of a branch of the flavonoid biosynthetic pathway. The MYB transcription factors (TF) act as activators or repressors to regulate the flavonoid pathway. In this study, 13 full-length cDNA clones of R2R3-MYB TFs from E. sagittatum (designated as EsMYB1 to EsMYB13) were isolated and characterized. Sequence similarity and phylogenetic analysis placed nine R2R3-MYB members of epimedii into five subgroups of the Arabidopsis R2R3-MYB family, while four members were not clustered into a defined subgroup. The number and length of introns from epimedii R2R3-MYB genes varied significantly, but intron positions and phases were well conserved. Expression patterns of epimedii R2R3-MYB genes in various tissues showed diverse. Finally, it is suggested that five epimedii R2R3-MYB genes may be involved in regulating the flavonoid pathway and could be used as valuable candidate genes for metabolic engineering studies in future. Sequence information of 13 R2R3-MYB genes discovered here will also provide an entry point into the overview of whole R2R3-MYB family in epimedii.

ACS Style

Wenjun Huang; Wei Sun; Haiyan Lv; Gong Xiao; Shaohua Zeng; Ying Wang. Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum. International Journal of Molecular Sciences 2012, 14, 594 -610.

AMA Style

Wenjun Huang, Wei Sun, Haiyan Lv, Gong Xiao, Shaohua Zeng, Ying Wang. Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum. International Journal of Molecular Sciences. 2012; 14 (1):594-610.

Chicago/Turabian Style

Wenjun Huang; Wei Sun; Haiyan Lv; Gong Xiao; Shaohua Zeng; Ying Wang. 2012. "Isolation and Molecular Characterization of Thirteen R2R3-MYB Transcription Factors from Epimedium sagittatum." International Journal of Molecular Sciences 14, no. 1: 594-610.

Journal article
Published: 27 November 2012 in Plant Cell, Tissue and Organ Culture (PCTOC)
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Phenylalanine ammonia-lyase (PAL) plays an important role in the phenylpropanoid pathway and in accumulation of major secondary metabolites in medicinal Epimedium species, including icariin, epimedin A, epimedin B, and epimedin C (hereafter designated as active components). In this study, three Epimedium sagittatum PALs (EsPALs) mRNA sequences, designated respectively as EsPAL1, EsPAL2 and EsPAL3 deduced to encode 708, 716, and 739 amino acids, were isolated and characterized. Based on sequence and phylogenetic analyses, EsPAL1 was found to be closer to EsPAL2 than to EsPAL3. Spatio-temporal expression profiles and metabolic accumulation profiles revealed that EsPAL3 was highly expressed in flavonoid-enriched tissues and leaves at certain developmental stages along with high levels of active components, while EsPAL1 was highly expressed in leathery leaves along with high lignin content. Under light stress, the total flavonoid content was enhanced by 100 μM phytohormones tested or 5 % sucrose through upregulating different EsPAL isoform(s). Our findings have laid a solid foundation for improving the content of bioactive components in Epimedium via metabolically engineering EsPAL.

ACS Style

Shaohua Zeng; Yilan Liu; Caiyun Zou; Wenjun Huang; Ying Wang. Cloning and characterization of phenylalanine ammonia-lyase in medicinal Epimedium species. Plant Cell, Tissue and Organ Culture (PCTOC) 2012, 113, 257 -267.

AMA Style

Shaohua Zeng, Yilan Liu, Caiyun Zou, Wenjun Huang, Ying Wang. Cloning and characterization of phenylalanine ammonia-lyase in medicinal Epimedium species. Plant Cell, Tissue and Organ Culture (PCTOC). 2012; 113 (2):257-267.

Chicago/Turabian Style

Shaohua Zeng; Yilan Liu; Caiyun Zou; Wenjun Huang; Ying Wang. 2012. "Cloning and characterization of phenylalanine ammonia-lyase in medicinal Epimedium species." Plant Cell, Tissue and Organ Culture (PCTOC) 113, no. 2: 257-267.