Small unmanned aircraft systems (UASs) are often suited to applications where the cost, resolution, and (or) operational inflexibility of conventional remote sensing platforms is limiting. Remote sensing with small UASs is still relatively new, and there is limited understanding of how the data are acquired and used for scientific purposes and decision making. This paper provides practical guidance about the opportunities and limitations of small UAS-based remote sensing by highlighting a small sample of scientific and commercial case studies. Case studies span four themes: (i) mapping, which includes case studies to measure aggregate stockpile volumes and map river habitat; (ii) feature detection, which includes case studies on grassland image classification and detection of agricultural crop infection; (iii) wildlife and animal enumeration, with case studies describing the detection of fish concentrations during a major salmon spawning event, and cattle enumeration at a concentrated animal feeding operation; (iv) landscape dynamics with a case study of arctic glacier change. Collectively, these case studies only represent a fraction of possible remote sensing applications using small UASs, but they provide insight into potential challenges and outcomes, and help clarify the opportunities and limitations that UAS technology offers for remote sensing of the environment.

Viruses preserved in ancient materials provide snapshots of past viral diversity and a means to trace viral evolution through time. Here, we use a metagenomics approach to identify filterable and nuclease-resistant nucleic acids preserved in 700-y-old caribou feces frozen in a permanent ice patch. We were able to recover and characterize two viruses in replicated experiments performed in two different laboratories: a small circular DNA viral genome (ancient caribou feces associated virus, or aCFV) and a partial RNA viral genome (Ancient Northwest Territories cripavirus, or aNCV). Phylogenetic analysis identifies aCFV as distantly related to the plant-infecting geminiviruses and the fungi-infecting Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 and aNCV as within the insect-infecting Cripavirus genus. We hypothesize that these viruses originate from plant material ingested by caribou or from flying insects and that their preservation can be attributed to protection within viral capsids maintained at cold temperatures. To investigate the tropism of aCFV, we used the geminiviral reverse genetic system and introduced a multimeric clone into the laboratory model plant Nicotiana benthamiana. Evidence for infectivity came from the detection of viral DNA in newly emerged leaves and the precise excision of the viral genome from the multimeric clones in inoculated leaves. Our findings indicate that viral genomes may in some circumstances be protected from degradation for centuries. Significance Knowledge of ancient viruses is limited due to their low concentration and poor preservation in ancient specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA and one partial RNA viral genome from a 700-y-old fecal sample preserved in ice. Using reverse genetics, we reconstituted the DNA virus, which replicated and systemically spread in a model plant species. Under constant freezing conditions, encapsidated viral nucleic acids may therefore be preserved for centuries. Our finding indicates that cryogenically preserved materials can be repositories of ancient viral nucleic acids, which in turn allow molecular genetics to regenerate viruses to study their biology.