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Background: Licorice is an important medicinal plant, but climate conditions can hinder seed production. Because it is difficult to produce the seeds, Korea mostly depends on the imported product. In addition, when grown domestically, the standard ingredients do not comply with those of the Korean Pharmacopoeia (glycyrrhizin 2.5% and liquritigenin 0.7%). Therefore, it is necessary to develop licorice variety suitable for the domestic environment. Methods and Results: A new interspecific hybrid cultivar designated ‘Dagam’, was developed from Glycyrrhiza glabra × G. uralensis (G. korshinskyi Grig.) and is regarded as a high-yielding and high-quality licorice by Rural Development Administration. On average the yield for ‘Dagam’ was recorded 431.5 ㎏/10a. The cultivar displayed strong brown spot disease and lodging resistance. Additionally, the glycyrrhizin and liquiritigenin contents of ‘Dagam’ (3.0 and 0.7%, respectively) met, the Korean Pharmacopoeia standard. This cultivar can widely adapt to most cultivation areas in Korea except Jejudo, Gangwondo, and the mountain-alpine regions. Furthermore, ‘Dagam’ was not showed any cytotoxicity and high cell viability compared to ‘G. uralensis’. Conclusions: The interspecific hybrid cultivar ‘Dagam’ will contribute to the stability of domestic cultivation of licorice in Korea.
Jeong Hoon Lee; Myeong Won Oh; Kyung Hye Seo; Chun Geun Park; Jin Tae Jeong; Jong Won Han; Kyung Ho Ma; Jae Ki Chang. Cultivation and Quality Characterization of ‘Dagam’, a Licorice Interspecific Hybrid Cultivar. Korean Journal of Medicinal Crop Science 2021, 29, 110 -116.
AMA StyleJeong Hoon Lee, Myeong Won Oh, Kyung Hye Seo, Chun Geun Park, Jin Tae Jeong, Jong Won Han, Kyung Ho Ma, Jae Ki Chang. Cultivation and Quality Characterization of ‘Dagam’, a Licorice Interspecific Hybrid Cultivar. Korean Journal of Medicinal Crop Science. 2021; 29 (2):110-116.
Chicago/Turabian StyleJeong Hoon Lee; Myeong Won Oh; Kyung Hye Seo; Chun Geun Park; Jin Tae Jeong; Jong Won Han; Kyung Ho Ma; Jae Ki Chang. 2021. "Cultivation and Quality Characterization of ‘Dagam’, a Licorice Interspecific Hybrid Cultivar." Korean Journal of Medicinal Crop Science 29, no. 2: 110-116.
Background: Safflower (Carthamus tinctorius L.) is a useful medicinal and oil crop in Korea. However, when safflower is cultivated, the flowering period overlaps with the rainy season, and seed maturation is poor. Therefore, this study aimed to use basic research data to develop superior varieties using agronomic characteristics and crossing method. Methods and Results: A total of 34 safflower germplasms were sown and their agronomic characteristics were investigated. Based on these investigations, the cultivar ‘ui-san-hong-hwa’ was selected as the mother plant, and ‘Myanmar safflower’ (Hsu Pan) was selected as the father plant. In addition, we developed a floret-protecting cap to cover florets after emasculation during the artificial crossing. When florets were protected by the cap, the seed setting rate increased in comparison to that in the non-covered florets. Conclusions: Agronomic characteristics can contribute to developing suitable varieties. The results suggest that the protection cap will be helpful in breeding without the floral organ drying. This study contributes an efficient breeding method to develop new safflower varieties.
Myeong Won Oh; Jeong Hoon Lee; Jin Tae Jeong; Jong Won Han; Sang Hoon Lee; Kyung Ho Ma; Mok Hur; Jae Ki Chang. Agronomic Characteristics and Artificial-cross Method of Collected Safflower (Carthamus tinctorius L.) Germplasm. Korean Journal of Medicinal Crop Science 2020, 28, 298 -309.
AMA StyleMyeong Won Oh, Jeong Hoon Lee, Jin Tae Jeong, Jong Won Han, Sang Hoon Lee, Kyung Ho Ma, Mok Hur, Jae Ki Chang. Agronomic Characteristics and Artificial-cross Method of Collected Safflower (Carthamus tinctorius L.) Germplasm. Korean Journal of Medicinal Crop Science. 2020; 28 (4):298-309.
Chicago/Turabian StyleMyeong Won Oh; Jeong Hoon Lee; Jin Tae Jeong; Jong Won Han; Sang Hoon Lee; Kyung Ho Ma; Mok Hur; Jae Ki Chang. 2020. "Agronomic Characteristics and Artificial-cross Method of Collected Safflower (Carthamus tinctorius L.) Germplasm." Korean Journal of Medicinal Crop Science 28, no. 4: 298-309.
Non-alcoholic steatohepatitis (NASH), a type of non-alcoholic fatty liver disease, is characterized as steatosis and inflammation in the liver. NLRP3 inflammasome activation is associated with NASH pathology. We hypothesized that suppressing the NLRP3 inflammasome could be effective in preventing NASH. We searched substances that could inhibit the activation of the NLRP3 inflammasome and identified sweroside as an NLRP3 inhibitor. We investigated whether sweroside can be applied to prevent the pathological symptoms associated with NASH in a methionine–choline-deficient (MCD) diet-induced NASH mouse model. The activation of the NLRP3 inflammasome was determined by detecting the production of caspase-1 and IL-1β from pro-caspase-1 and pro-IL-1β in primary mouse macrophages and mouse liver. In a NASH model, mice were fed an MCD diet for two weeks with daily intraperitoneal injections of sweroside. Sweroside effectively inhibited NLRP3 inflammasome activation in primary macrophages as shown by a decrease in IL-1β and caspase-1 production. In a MCD diet-induced NASH mouse model, intraperitoneal injection of sweroside significantly reduced serum aspartate transaminase and alanine transaminase levels, hepatic immune cell infiltration, hepatic triglyceride accumulation, and liver fibrosis. The improvement of NASH symptoms by sweroside was accompanied with its inhibitory effects on the hepatic NLRP3 inflammasome as hepatic IL-1β and caspase-1 were decreased. Furthermore, sweroside blocked de novo synthesis of mitochondrial DNA in the liver, contributing to suppression of the NLRP3 inflammasome. These results suggest that targeting the NLRP3 inflammasome with sweroside could be beneficially employed to improve NASH symptoms.
Gabsik Yang; Joo Hyeon Jang; Sung Wook Kim; Sin-Hee Han; Kyung-Ho Ma; Jae-Ki Jang; Han Chang Kang; Yong-Yeon Cho; Hye Suk Lee; Joo Young Lee. Sweroside Prevents Non-Alcoholic Steatohepatitis by Suppressing Activation of the NLRP3 Inflammasome. International Journal of Molecular Sciences 2020, 21, 2790 .
AMA StyleGabsik Yang, Joo Hyeon Jang, Sung Wook Kim, Sin-Hee Han, Kyung-Ho Ma, Jae-Ki Jang, Han Chang Kang, Yong-Yeon Cho, Hye Suk Lee, Joo Young Lee. Sweroside Prevents Non-Alcoholic Steatohepatitis by Suppressing Activation of the NLRP3 Inflammasome. International Journal of Molecular Sciences. 2020; 21 (8):2790.
Chicago/Turabian StyleGabsik Yang; Joo Hyeon Jang; Sung Wook Kim; Sin-Hee Han; Kyung-Ho Ma; Jae-Ki Jang; Han Chang Kang; Yong-Yeon Cho; Hye Suk Lee; Joo Young Lee. 2020. "Sweroside Prevents Non-Alcoholic Steatohepatitis by Suppressing Activation of the NLRP3 Inflammasome." International Journal of Molecular Sciences 21, no. 8: 2790.
Black soybean (Glycine max L.) has been used as a traditional medicine because its seed coat contains various natural phenolic compounds such as anthocyanins. The objective of this study was to reveal the genetic variation in the agricultural traits, phytochemicals, and antioxidant activity of 172 Korean black soybean landraces (KBSLs) and establish a relationship among them. The evaluation of three agricultural traits (days to 50% flowering, maturity, and 100-seed weight), six phytochemicals (delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside, daidzin, glycitin, and genestin), and four antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), ferric-reducing antioxidant power (FRAP), and the total polyphenol content (TPC) of 172 KBSLs were analyzed in 2012 and 2015. The agricultural traits, phytochemicals, and antioxidant activities of the 172 KBSLs showed wide variation among the accessions and years. In correlation analysis, the agricultural traits and phytochemicals showed positive and negative correlations with phytochemicals and antioxidant activity, respectively. The principal component analyses result indicated that phytochemicals accounted for most of the variability in the KBSLs. In clustering analysis, the 172 KBSLs were classified into four clusters. These results could lead to expanding the knowledge of the agricultural traits, phytochemicals, and antioxidant activity of the KBSLs, which are valuable materials for the development of new soybean varieties.
Kyung Jun Lee; Da-Young Baek; Gi-An Lee; Gyu-Taek Cho; Yoon-Sup So; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung; Do Yoon Hyun. Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces. Antioxidants 2020, 9, 213 .
AMA StyleKyung Jun Lee, Da-Young Baek, Gi-An Lee, Gyu-Taek Cho, Yoon-Sup So, Jung-Ro Lee, Kyung-Ho Ma, Jong-Wook Chung, Do Yoon Hyun. Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces. Antioxidants. 2020; 9 (3):213.
Chicago/Turabian StyleKyung Jun Lee; Da-Young Baek; Gi-An Lee; Gyu-Taek Cho; Yoon-Sup So; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung; Do Yoon Hyun. 2020. "Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces." Antioxidants 9, no. 3: 213.
Background: This study was conducted to compare the physicochemical properties of 12 different cultivars of Rehmannia glutinosa, a plant used in Korean traditional herbal medicine. Method and Results: We investigated the following 12 cultivars of R. glutinosa; Jihwang1, Goryeo, Daegyung, Gogang, Togang, Dagang, Wongang, Yeongang, Hwanggang, Dahwang, Segang and Chunggang. We measured water content, chromaticity, hardness, active components levels, and antioxidant activity. Our results showed that Togang has the lowest water content and the highest yield. The catalpol content was the highest in Togang (40.50 ㎎/g) and the lowest in Chunggang (22.17 ㎎/g). The aucubin content was the highest in Hwanggang (0.28 ㎎/g). Chunggang had the lowest aucubin content, but the highest level of verbascoside. Total flavonoid and total polyphenol were the highest in Daegyung and Chunggang, respectively. There was a strong correlation between the total flavonoid and total polyphenol (r = 0.89) content. Conclusions: There were significant differences in physicochemical properties among 12 cultivars. These properties will affect suitability for processing and final product quality. We therefore suggest the selection of appropriate cultivars for different uses.
Yae Jin Kim; Sin Hee Han; Chung Oui Hong; Jong Won Han; Sang Hoon Lee; Jae Ki Chang; Kyung Ho Ma. Comparison of Physicochemical Properties on Dried Rehmannia glutinosa with Different Cultivars. Korean Journal of Medicinal Crop Science 2019, 27, 330 -338.
AMA StyleYae Jin Kim, Sin Hee Han, Chung Oui Hong, Jong Won Han, Sang Hoon Lee, Jae Ki Chang, Kyung Ho Ma. Comparison of Physicochemical Properties on Dried Rehmannia glutinosa with Different Cultivars. Korean Journal of Medicinal Crop Science. 2019; 27 (5):330-338.
Chicago/Turabian StyleYae Jin Kim; Sin Hee Han; Chung Oui Hong; Jong Won Han; Sang Hoon Lee; Jae Ki Chang; Kyung Ho Ma. 2019. "Comparison of Physicochemical Properties on Dried Rehmannia glutinosa with Different Cultivars." Korean Journal of Medicinal Crop Science 27, no. 5: 330-338.
Background: Various Schisandra chinensis (SC) varieties grow in diverse regions in Korea. However, there is no valid scientific evidence of these varieties. This study aimed to select the excellent resources in terms of the growth characteristics, antioxidant activities, and analysis of the active compounds of the SC collection. Method and Results: In total, 154 resources of SC were collected from various regions of Korea. The growth characteristics were measured by the number of fruit bunches, fruit number, and weight of 100 fruits. The antioxidant activities were investigated by analyzing the total flavonoid and total polyphenol contents and the radical scavenging activity of DPPH and ABTS. Schizandrin A, schizandrin c, gomisin a, and gomisin N were analyzed by HPLC. Each resource showed different growth characteristics. Among the antioxidative effects, the highest 20 resources showed high antioxidant activities in selected 29 resources. Analysis of the SC lignan index showed that all resources contained more than 1.16% of active compounds. Conclusions: All of the selected 29 SC resources were shown to have excellent growth characteristics, antioxidant activities, and bioactive compound richness. Especially, SC-004, SC-007, and SC-154 showed the best growth characteristics, and SC-22, SC-40, and SC-45 showed the best antioxidant activities and bioactive compound richness.
Sin Hee Han; Jae Ki Jang; Kyung Ho Ma; Yae Jin Kim; Seon Mi Kim; Hee Jung Lee; Chung Oui Hong. Selection of Superior Resources through Analysis of Growth Characteristics and Physiological Activity of Schisandra chinensis Collection. Korean Journal of Medicinal Crop Science 2019, 27, 9 -16.
AMA StyleSin Hee Han, Jae Ki Jang, Kyung Ho Ma, Yae Jin Kim, Seon Mi Kim, Hee Jung Lee, Chung Oui Hong. Selection of Superior Resources through Analysis of Growth Characteristics and Physiological Activity of Schisandra chinensis Collection. Korean Journal of Medicinal Crop Science. 2019; 27 (1):9-16.
Chicago/Turabian StyleSin Hee Han; Jae Ki Jang; Kyung Ho Ma; Yae Jin Kim; Seon Mi Kim; Hee Jung Lee; Chung Oui Hong. 2019. "Selection of Superior Resources through Analysis of Growth Characteristics and Physiological Activity of Schisandra chinensis Collection." Korean Journal of Medicinal Crop Science 27, no. 1: 9-16.
Kyung-Ho Ma; Mun-Sup Yoon; Kyung Jun Lee; Hari Deo Upadhyaya; Doo-Yeon Kwak; Ji-Yeon Ko; Hyung Jin Baek. Establishment and Utilization of Core and Mini Core Collections of the Mandated Crops at the International Crops Research Institute for the Semi-Arid Tropics(ICRISAT). Journal of the Korean Society of International Agriculture 2018, 30, 184 -192.
AMA StyleKyung-Ho Ma, Mun-Sup Yoon, Kyung Jun Lee, Hari Deo Upadhyaya, Doo-Yeon Kwak, Ji-Yeon Ko, Hyung Jin Baek. Establishment and Utilization of Core and Mini Core Collections of the Mandated Crops at the International Crops Research Institute for the Semi-Arid Tropics(ICRISAT). Journal of the Korean Society of International Agriculture. 2018; 30 (3):184-192.
Chicago/Turabian StyleKyung-Ho Ma; Mun-Sup Yoon; Kyung Jun Lee; Hari Deo Upadhyaya; Doo-Yeon Kwak; Ji-Yeon Ko; Hyung Jin Baek. 2018. "Establishment and Utilization of Core and Mini Core Collections of the Mandated Crops at the International Crops Research Institute for the Semi-Arid Tropics(ICRISAT)." Journal of the Korean Society of International Agriculture 30, no. 3: 184-192.
Little millet, Panicum sumatrense Roth ex Roem. & Schult., is an important cultivated species under the tribe Paniceae, sub-family Panicoideae and family Poaceae. In this study, for the first time we sequenced the complete chloroplast (cp) genome of P. sumatrense to investigate their phylogenetic relationship in the family Poaceae. The complete cp genome sequence of P. sumatrense is 139,384 bp in length with 38.6% overall GC content and exhibits a typical quadripartite structure comprising one pair of inverted repeats (22,723 bp) separated by a small single-copy region (12,583 bp) and a large single-copy region (81,355 bp). The P. sumatrense cp genome encodes 125 unique genes, which include 91 protein-coding genes, 4 rRNA genes, 30 tRNA genes, and 20 genes were duplicated in the inverted repeat region. This newly determined cp genome (P. sumatrense) could be valuable information for the breeding programs of this cereal crops in the family Poaceae.
Raveendar Sebastin; Gi-An Lee; Kyung Jun Lee; Myoung-Jae Shin; Gyu-Taek Cho; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung. The complete chloroplast genome sequences of little millet (Panicum sumatrense Roth ex Roem. and Schult.) (Poaceae). Mitochondrial DNA Part B 2018, 3, 719 -720.
AMA StyleRaveendar Sebastin, Gi-An Lee, Kyung Jun Lee, Myoung-Jae Shin, Gyu-Taek Cho, Jung-Ro Lee, Kyung-Ho Ma, Jong-Wook Chung. The complete chloroplast genome sequences of little millet (Panicum sumatrense Roth ex Roem. and Schult.) (Poaceae). Mitochondrial DNA Part B. 2018; 3 (2):719-720.
Chicago/Turabian StyleRaveendar Sebastin; Gi-An Lee; Kyung Jun Lee; Myoung-Jae Shin; Gyu-Taek Cho; Jung-Ro Lee; Kyung-Ho Ma; Jong-Wook Chung. 2018. "The complete chloroplast genome sequences of little millet (Panicum sumatrense Roth ex Roem. and Schult.) (Poaceae)." Mitochondrial DNA Part B 3, no. 2: 719-720.
Near infrared reflectance spectroscopy (NIRS), a non-destructive and rapid analytical method, was used to examine the possibility of replacing a method for the large-scale screening of tomato seed viability. A total of 368 tomato seed samples were used for development and validation of an NIRS calibration model. The accelerating aging method (98 ± 2% R.H., 40 °C) was employed for preparation of a calibration set (n = 268) and a validation set (n = 100) with wider seed viability. Among the tomato NIRS calibration models tested, the modified partial least square (MPLS) regression produced the best equation model. Specifically, this model produced a higher RSQ (0.9446) and lower SEC (6.5012) during calibration and a higher 1-VR (0.9194) and lower SECV (7.8264) upon cross-validation compared to the other regression methods (PLS, PCR) tested in this study. Additionally, the SD/SECV was 3.53, which was greater than the criterion point of 3. External validation of this NIRS equation revealed a significant correlation between reference values and NIRS-estimated values based on the coefficient of determination (R2), the standard error of prediction (SEP (C)), and the ratio of performance to deviation (RPD = SD/SEP (C)), which were 0.94, 6.57, and 3.96, respectively. The external validation demonstrated that this model had predictive accuracy in tomato, indicating that it has the potential to replace the germination test.
Ho-Sun Lee; Young-Ah Jeon; Young-Yi Lee; Gi-An Lee; Sebastin Raveendar; Kyung Ho Ma. Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS). Sustainability 2017, 9, 618 .
AMA StyleHo-Sun Lee, Young-Ah Jeon, Young-Yi Lee, Gi-An Lee, Sebastin Raveendar, Kyung Ho Ma. Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS). Sustainability. 2017; 9 (4):618.
Chicago/Turabian StyleHo-Sun Lee; Young-Ah Jeon; Young-Yi Lee; Gi-An Lee; Sebastin Raveendar; Kyung Ho Ma. 2017. "Large-Scale Screening of Intact Tomato Seeds for Viability Using Near Infrared Reflectance Spectroscopy (NIRS)." Sustainability 9, no. 4: 618.
Improving eating quality and nutrition is one of the most important objectives in rice breeding program. Palatability factors and seed protein patterns of 69 Korean rice landraces were compared in this study. Of seven palatability factors, setback viscosity (SBV) had the largest variance, ranging from -144 to 45. Water content (WC) had the lowest variance, ranging from 11.0 to 13.0. In correlation analysis, eating quality was positively correlated with all palatability factors except gelatinization temperature (GT). In principal component analyses (PCA), the first PC with Eigen value of 5.06 explained 42.2% of the total variance. Cool paste viscosity (CPV) was the variable that had the largest positive loading. The second PC with Eigen value of 2.09 explained an additional 17.4% of the total variance. SBV was a variable that had the highest negative loading. Peak viscosity (PKV) had positive variance between PC1 and PC2. The 69 Korean rice landraces were clustered into four groups based on physicochemical properties and palatability factors, with groups I and II showing higher EQ than other groups. SDS-PAGE analyses revealed five seed storage patterns in the 69 Korean rice landraces. Among them, 84% (58 landraces) had pattern I. These results indicate that it is possible to develop high palatability cultivars using Korean rice landraces. In addition, screening of landraces based on variation in seed storage protein profile using SDS-PAGE could be highly effective for the identification of valuable rice genetic resources.
Kyung Jun Lee; Gi-An Lee; Jung-Ro Lee; Sebastin Raveendar; Yang-Hee Cho; Sok-Young Lee; Jong-Wook Chung; Kyung-Ho Ma. Comparison of eating quality and seed storage protein among Korean rice landraces. Journal of Crop Science and Biotechnology 2016, 19, 241 -247.
AMA StyleKyung Jun Lee, Gi-An Lee, Jung-Ro Lee, Sebastin Raveendar, Yang-Hee Cho, Sok-Young Lee, Jong-Wook Chung, Kyung-Ho Ma. Comparison of eating quality and seed storage protein among Korean rice landraces. Journal of Crop Science and Biotechnology. 2016; 19 (3):241-247.
Chicago/Turabian StyleKyung Jun Lee; Gi-An Lee; Jung-Ro Lee; Sebastin Raveendar; Yang-Hee Cho; Sok-Young Lee; Jong-Wook Chung; Kyung-Ho Ma. 2016. "Comparison of eating quality and seed storage protein among Korean rice landraces." Journal of Crop Science and Biotechnology 19, no. 3: 241-247.
Sebastin Raveendar; Jong-Wook Chung; Gi-An Lee; Jung-Ro Lee; Kyung-Jun Lee; Myoung-Jae Shin; Yang-Hee Cho; Kyung-Ho Ma. Genetic Diversity and Population Structure of Asian Tomato Accessions Based on Simple-Sequence Repeats. Plant Breeding and Biotechnology 2016, 4, 306 -314.
AMA StyleSebastin Raveendar, Jong-Wook Chung, Gi-An Lee, Jung-Ro Lee, Kyung-Jun Lee, Myoung-Jae Shin, Yang-Hee Cho, Kyung-Ho Ma. Genetic Diversity and Population Structure of Asian Tomato Accessions Based on Simple-Sequence Repeats. Plant Breeding and Biotechnology. 2016; 4 (3):306-314.
Chicago/Turabian StyleSebastin Raveendar; Jong-Wook Chung; Gi-An Lee; Jung-Ro Lee; Kyung-Jun Lee; Myoung-Jae Shin; Yang-Hee Cho; Kyung-Ho Ma. 2016. "Genetic Diversity and Population Structure of Asian Tomato Accessions Based on Simple-Sequence Repeats." Plant Breeding and Biotechnology 4, no. 3: 306-314.
Kyung Jun Lee; Gi-An Lee; Hee-Kyoung Kang; Jung-Ro Lee; Sebastin Raveendar; Myoung-Jae Shin; Yang-Hee Cho; Kyung-Ho Ma. Genetic Diversity and Population Structure of Rubus Accessions Using Simple Sequence Repeat Markers. Plant Breeding and Biotechnology 2016, 4, 345 -351.
AMA StyleKyung Jun Lee, Gi-An Lee, Hee-Kyoung Kang, Jung-Ro Lee, Sebastin Raveendar, Myoung-Jae Shin, Yang-Hee Cho, Kyung-Ho Ma. Genetic Diversity and Population Structure of Rubus Accessions Using Simple Sequence Repeat Markers. Plant Breeding and Biotechnology. 2016; 4 (3):345-351.
Chicago/Turabian StyleKyung Jun Lee; Gi-An Lee; Hee-Kyoung Kang; Jung-Ro Lee; Sebastin Raveendar; Myoung-Jae Shin; Yang-Hee Cho; Kyung-Ho Ma. 2016. "Genetic Diversity and Population Structure of Rubus Accessions Using Simple Sequence Repeat Markers." Plant Breeding and Biotechnology 4, no. 3: 345-351.
The vetch (Vicia sativa) is one of the most important annual forage legumes globally due to its multiple uses and high nutritional content. Despite these agronomical benefits, many drawbacks, including cyano-alanine toxin, has reduced the agronomic value of vetch varieties. Here, we used 454 technology to sequence the two V. sativa subspecies (ssp. sativa and ssp. nigra) to enrich functional information and genetic marker resources for the vetch research community. A total of 86,532 and 47,103 reads produced 35,202 and 18,808 unigenes with average lengths of 735 and 601 bp for V. sativa sativa and V. sativa nigra, respectively. Gene Ontology annotations and the cluster of orthologous gene classes were used to annotate the function of the Vicia transcriptomes. The Vicia transcriptome sequences were then mined for simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. About 13% and 3% of the Vicia unigenes contained the putative SSR and SNP sequences, respectively. Among those SSRs, 100 were chosen for the validation and the polymorphism test using the Vicia germplasm set. Thus, our approach takes advantage of the utility of transcriptomic data to expedite a vetch breeding program.
Tae-Sung Kim; Sebastin Raveendar; Sundan Suresh; Gi-An Lee; Jung-Ro Lee; Joon-Hyeong Cho; Sok-Young Lee; Kyung-Ho Ma; Gyu-Taek Cho; Jong-Wook Chung. Transcriptome Analysis of Two Vicia sativa Subspecies: Mining Molecular Markers to Enhance Genomic Resources for Vetch Improvement. Genes 2015, 6, 1164 -1182.
AMA StyleTae-Sung Kim, Sebastin Raveendar, Sundan Suresh, Gi-An Lee, Jung-Ro Lee, Joon-Hyeong Cho, Sok-Young Lee, Kyung-Ho Ma, Gyu-Taek Cho, Jong-Wook Chung. Transcriptome Analysis of Two Vicia sativa Subspecies: Mining Molecular Markers to Enhance Genomic Resources for Vetch Improvement. Genes. 2015; 6 (4):1164-1182.
Chicago/Turabian StyleTae-Sung Kim; Sebastin Raveendar; Sundan Suresh; Gi-An Lee; Jung-Ro Lee; Joon-Hyeong Cho; Sok-Young Lee; Kyung-Ho Ma; Gyu-Taek Cho; Jong-Wook Chung. 2015. "Transcriptome Analysis of Two Vicia sativa Subspecies: Mining Molecular Markers to Enhance Genomic Resources for Vetch Improvement." Genes 6, no. 4: 1164-1182.
Chloroplast (cp) genome sequences provide a valuable source for DNA barcoding. Molecular phylogenetic studies have concentrated on DNA sequencing of conserved gene loci. However, this approach is time consuming and more difficult to implement when gene organization differs among species. Here we report the complete re-sequencing of the cp genome of Capsicum pepper (Capsicum annuum var. glabriusculum) using the Illumina platform. The total length of the cp genome is 156,817 bp with a 37.7% overall GC content. A pair of inverted repeats (IRs) of 50,284 bp were separated by a small single copy (SSC; 18,948 bp) and a large single copy (LSC; 87,446 bp). The number of cp genes in C. annuum var. glabriusculum is the same as that in other Capsicum species. Variations in the lengths of LSC; SSC and IR regions were the main contributors to the size variation in the cp genome of this species. A total of 125 simple sequence repeat (SSR) and 48 insertions or deletions variants were found by sequence alignment of Capsicum cp genome. These findings provide a foundation for further investigation of cp genome evolution in Capsicum and other higher plants.
Sebastin Raveendar; Young-Wang Na; Jung-Ro Lee; Donghwan Shim; Kyung-Ho Ma; Sok-Young Lee; Jong-Wook Chung. The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina Sequencing. Molecules 2015, 20, 13080 -13088.
AMA StyleSebastin Raveendar, Young-Wang Na, Jung-Ro Lee, Donghwan Shim, Kyung-Ho Ma, Sok-Young Lee, Jong-Wook Chung. The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina Sequencing. Molecules. 2015; 20 (7):13080-13088.
Chicago/Turabian StyleSebastin Raveendar; Young-Wang Na; Jung-Ro Lee; Donghwan Shim; Kyung-Ho Ma; Sok-Young Lee; Jong-Wook Chung. 2015. "The Complete Chloroplast Genome of Capsicum annuum var. glabriusculum Using Illumina Sequencing." Molecules 20, no. 7: 13080-13088.
The Rubus genus consists of more than 600 species that are distributed globally. Only a few Rubus species, including raspberries and blueberries, have been domesticated. Genetic diversity within and between Rubus species is an important resource for breeding programs. We developed genomic microsatellite markers using an SSR-enriched R. coreanus library to study the diversity of the Rubus species. Microsatellite motifs were discovered in 546 of 646 unique clones, and a dinucleotide repeat was the most frequent (75.3%) type of repeat. From 97 microsatellite loci with reproducible amplicons, we acquired 29 polymorphic microsatellite markers in the Rubus coreanus collection. The transferability values ranged from 59.8% to 84% across six Rubus species, and Rubus parvifolius had the highest transferability value (84%). The average number of alleles and the polymorphism information content were 5.7 and 0.541, respectively, in the R. coreanus collection. The diversity index of R. coreanus was similar to the values reported for other Rubus species. A phylogenetic dendrogram based on SSR profiles revealed that seven Rubus species could be allocated to three groups, and that R. coreanus was genetically close to Rubus crataegifolius (mountain berry). These new microsatellite markers might prove useful in studies of the genetic diversity, population structure, and evolutionary relationships among Rubus species.
Gi-An Lee; Jae Young Song; Heh-Ran Choi; Jong-Wook Chung; Young-Ah Jeon; Jung-Ro Lee; Kyung-Ho Ma; Myung-Chul Lee. Novel Microsatellite Markers Acquired from Rubus coreanus Miq. and Cross-Amplification in Other Rubus Species. Molecules 2015, 20, 6432 -6442.
AMA StyleGi-An Lee, Jae Young Song, Heh-Ran Choi, Jong-Wook Chung, Young-Ah Jeon, Jung-Ro Lee, Kyung-Ho Ma, Myung-Chul Lee. Novel Microsatellite Markers Acquired from Rubus coreanus Miq. and Cross-Amplification in Other Rubus Species. Molecules. 2015; 20 (4):6432-6442.
Chicago/Turabian StyleGi-An Lee; Jae Young Song; Heh-Ran Choi; Jong-Wook Chung; Young-Ah Jeon; Jung-Ro Lee; Kyung-Ho Ma; Myung-Chul Lee. 2015. "Novel Microsatellite Markers Acquired from Rubus coreanus Miq. and Cross-Amplification in Other Rubus Species." Molecules 20, no. 4: 6432-6442.
The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.
Sebastin Raveendar; Gi-An Lee; Young-Ah Jeon; Yun Jeong Lee; Jung-Ro Lee; Gyu-Taek Cho; Joon-Hyeong Cho; Jong-Hyun Park; Kyung-Ho Ma; Jong-Wook Chung. Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species. Molecules 2015, 20, 1543 -1550.
AMA StyleSebastin Raveendar, Gi-An Lee, Young-Ah Jeon, Yun Jeong Lee, Jung-Ro Lee, Gyu-Taek Cho, Joon-Hyeong Cho, Jong-Hyun Park, Kyung-Ho Ma, Jong-Wook Chung. Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species. Molecules. 2015; 20 (1):1543-1550.
Chicago/Turabian StyleSebastin Raveendar; Gi-An Lee; Young-Ah Jeon; Yun Jeong Lee; Jung-Ro Lee; Gyu-Taek Cho; Joon-Hyeong Cho; Jong-Hyun Park; Kyung-Ho Ma; Jong-Wook Chung. 2015. "Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species." Molecules 20, no. 1: 1543-1550.
Ho-Sun Lee; Jang-Won Lee; Su-Jung Kim; Jeong-Hoon Lee; Jung-Sook Sung; Man-Jung Kang; Kyung-Ho Ma. Effects of Temperature, Light and Chemical Reagent on Dormancy Breaking and Seed Germination of Three Species in Apiaceae. Journal of the Korean Society of International Agriculture 2014, 26, 519 -525.
AMA StyleHo-Sun Lee, Jang-Won Lee, Su-Jung Kim, Jeong-Hoon Lee, Jung-Sook Sung, Man-Jung Kang, Kyung-Ho Ma. Effects of Temperature, Light and Chemical Reagent on Dormancy Breaking and Seed Germination of Three Species in Apiaceae. Journal of the Korean Society of International Agriculture. 2014; 26 (4):519-525.
Chicago/Turabian StyleHo-Sun Lee; Jang-Won Lee; Su-Jung Kim; Jeong-Hoon Lee; Jung-Sook Sung; Man-Jung Kang; Kyung-Ho Ma. 2014. "Effects of Temperature, Light and Chemical Reagent on Dormancy Breaking and Seed Germination of Three Species in Apiaceae." Journal of the Korean Society of International Agriculture 26, no. 4: 519-525.
Soon-Jae Kwon; Gi-An Lee; Yong-Bum Kwack; Ho-Sun Lee; Gyu-Taek Cho; Ho-Cheol Ko; Sok-Young Lee; Yeon-Gyu Kim; Kyung-Ho Ma. Development of 34 New Microsatellite Markers from Actinidia arguta: Intra- and Interspecies Genetic Analysis. Plant Breeding and Biotechnology 2013, 1, 137 -147.
AMA StyleSoon-Jae Kwon, Gi-An Lee, Yong-Bum Kwack, Ho-Sun Lee, Gyu-Taek Cho, Ho-Cheol Ko, Sok-Young Lee, Yeon-Gyu Kim, Kyung-Ho Ma. Development of 34 New Microsatellite Markers from Actinidia arguta: Intra- and Interspecies Genetic Analysis. Plant Breeding and Biotechnology. 2013; 1 (2):137-147.
Chicago/Turabian StyleSoon-Jae Kwon; Gi-An Lee; Yong-Bum Kwack; Ho-Sun Lee; Gyu-Taek Cho; Ho-Cheol Ko; Sok-Young Lee; Yeon-Gyu Kim; Kyung-Ho Ma. 2013. "Development of 34 New Microsatellite Markers from Actinidia arguta: Intra- and Interspecies Genetic Analysis." Plant Breeding and Biotechnology 1, no. 2: 137-147.