Recently, neonicotinoids have become the fastest-growing class of insecticides in conventional crop protection, with extensive usage against a wide range of sucking and chewing pests. Neonicotinoids are widely used due to their high toxicity to invertebrates, simplicity, flexibility with which they may be applied, and lengthy persistence, and their systemic nature ensures that they spread to all sections of the target crop. However, these properties raise the risk of environmental contaminations and potential toxicity to non-target organisms. Acetamiprid is a new generation insecticide, which is a safer alternative for controlling insect pests because of its low toxicity to honeybees. Acetamiprid is intended to target nicotinic acetylcholine receptors in insects, but its widespread usage has resulted in negative impacts on non-target animals such as mammals. This review summarizes in vivo and in vitro animal studies that investigated the toxicity of specific neonicotinoids. With summarized data, it can be presumed that certain concentrations of neonicotinoids in the reproductive system cause oxidative stress in the testis; spermatogenesis disruption; spermatozoa degradation; interruptions to endocrine function and Sertoli and Leydig cell function. In the female reproductive system, acetamiprid evokes pathomorphological alterations in follicles, along with metabolic changes in the ovaries.

The alarming growth of chronic diseases is a major problem in Europe, and current data suggest an even greater burden in the future. Although genetic predispositions remain important determinants in the development of certain disorders, the appropriate diet can considerably minimize the risk of many diseases. Many plant species do, in fact, have health-promoting properties due to their high levels of physiologically active chemicals. Glucosinolates, vitamins, tocopherols, saponins, tannins, and other polyphenols have been shown to have beneficial impacts on human health, including cancer, diabetes, cardiovascular, kidney, and Alzheimer's disease prevention. A new plant category known as microgreens has evolved as a rich source of vitamins, minerals, carotenoids, and physiologically active chemicals against these backdrops. Microgreens could have 10 to 100 times more effective phytochemical concentrations than adult equivalents. Furthermore, they are environmentally cultured in regulated environments with no dirt, harmful residues, or excessive water use. However, existing limitations include uncertain methods of action, varying bioaccessibility, and a paucity of published clinical research. Indeed, microgreens could be a viable new food source for people who are interested in consuming healthy diets.

The objective of present study was to investigate in vitro protective potential of resveratrol in TM3 Leydig cells with induced oxidative stress using hydrogen peroxide (H₂O₂). Leydig cells experiencing oxidative stress exhibit reduced activities in androgens production, and become hypofunctional with age, which is also related to growing oxidative stress, while resveratrol has received growing attention as a cytoprotective agent. TM3 mouse Leydig cells were cultivated during 24 h in the presence of resveratrol (5, 10, 25, 50 and 100 μM) alone, or in combination with H₂O₂ (300/600 μM) to induce oxidative stress. Mitochondrial activity was evaluated using MTT test, triple assay was used in order to assess cell viability parameters, intracellular generation of superoxide was determined by the nitroblue-tetrazolium assay, and quantification of steroid hormones was performed by the enzyme- linked immunosorbent assay. Resveratrol alone treatment led to the most significantly improved values of all tested parameters in the cells of experimental group with addition of 10 μM of resveratrol in comparison to the control group. In the case of cells with induced oxidative stress (300 μM H₂O₂) resveratrol administration resulted in significantly increased (P < 0.05) metabolic activity, as well as cell membrane integrity at concentration 10 μM. Significantly improved (P < 0.001) lysosomal activity showed cells treated with 5 and 10 μM of resveratrol, and the level of both measured hormones was significantly higher (P < 0.05) in cells supplemented with 10 μM of resveratrol. Significant decline of superoxide radical production was observed in all experimental groups in comparison to the control exposed to H₂O₂ alone. With respect to cells exposed to higher concentration of H₂O₂ (600 μM)_, results showed positive effect of resveratrol only in biosynthesis of both androgens with significant increased values in experimental group treated with 5 μM (P < 0.05) and 10 μM (P < 0.01) of resveratrol, in addition, in the case of testosterone we recorded significant higher (P < 0.05) values in cells with addition of 25 and 50 μM resveratrol when compared to H₂O₂ control. More specific and systematic research focused especially on androgen biosynthesis is necessary related to the biological activity of resveratrol in male reproductive system due to inconsistent results of studies.