With global warming, the large amount of greenhouse gas emissions released by permafrost degradation is important in the global carbon and nitrogen cycle. To study the feedback effect of greenhouse gases on climate change in permafrost regions, emissions of CO₂, CH₄, and N₂O were continuously measured by using the static chamber-gas chromatograph method, in three forest soil ecosystems (Larix gmelinii, Pinus sylvestris var. mongolica, and Betula platyphylla) of the Daxing’an Mountains, northeast China, from May 2016 to April 2018. Their dynamic characteristics, as well as the key environmental affecting factors, were also analyzed. The results showed that the flux variation ranges of CO₂, CH₄, and N₂O were 7.92 ± 1.30~650.93 ± 28.12 mg·m⁻²·h⁻¹, −57.71 ± 4.65~32.51 ± 13.03 ug·m⁻²·h⁻¹, and −3.87 ± 1.35~31.1 ± 2.92 ug·m⁻²·h⁻¹, respectively. The three greenhouse gas fluxes showed significant seasonal variations, and differences in soil CO₂ and CH₄ fluxes between different forest types were significant. The calculation fluxes indicated that the permafrost soil of the Daxing’an Mountains may be a potential source of CO₂ and N₂O, and a sink of CH₄. Each greenhouse gas was controlled using different key environmental factors. Based on the analysis of Q₁₀ values and global warming potential, the obtained results demonstrated that greenhouse gas emissions from forest soil ecosystems in the permafrost region of the Daxing’an Mountains, northeast China, promote the global greenhouse effect.

Permafrost degradation by global warming is expected to alter the hydrological processes, which results in changes in vegetation species composition and gives rise to community succession. Ecotones are sensitive transition areas between ecosystem boundaries, attract particular interest due to their ecological importance and prompt responses to the environmental variables. However, the characteristics of soil microbial communities and extracellular enzymes along the forest–wetland ecotone in high‐latitude permafrost region remain poorly understood. In this study, we evaluated the variations of soil bacterial and fungal community structures and soil extracellular enzymatic activities of 0–10 cm and 10–20 cm soil layers in five different wetland types along environmental gradients, including Larix gmelinii swamp (LY), Betula platyphylla swamp (BH), Alnus sibirica var. hirsute swamp (MCY), thicket swamp (GC), and tussock swamp (CC). The relative abundances of some dominant bacterial (Actinobacteria and Verrucomicrobia) and fungal (Ascomycota and Basidiomycota) phyla differed significantly among different wetlands, while bacterial and fungal alpha diversity was not strongly affected by soil depth. PCoA results showed that vegetation type, rather than soil depth explained more variation of soil microbial community structure. β‐glucosidase and β‐N‐acetylglucosaminidase activities were significantly lower in GC and CC than in LY, BH, and MCY, while acid phosphatase activity was significantly higher in BH and GC than LY and CC. Altogether, the data suggest that soil moisture content (SMC) was the most important environmental factor contributing to the bacterial and fungal communities, while extracellular enzymatic activities were closely related to soil total organic carbon (TOC), nitrate nitrogen () and total phosphorus (TP).

Permafrost peatlands are a huge carbon pool that is uniquely sensitive to global warming. However, despite the importance of peatlands in global carbon sequestration and biogeochemical cycles, few studies have characterized the distribution characteristics and drivers of soil microbial community structure in forest–peatland ecotones. Here, we investigated the vertical distribution patterns of soil microbial communities in three typical peatlands along an environmental gradient using Illumina high-throughput sequencing. Our findings indicated that bacterial richness and diversity decreased with increasing soil depth in coniferous swamp (LT) and thicket swamp (HT), whereas the opposite trend was observed in a tussock swamp (NT). Additionally, these parameters decreased at 0–20 and 20–40 cm and increased at 40–60 cm along the environmental gradient (LT to NT). Principal coordinate analysis (PCoA) indicated that the soil microbial community structure was more significantly affected by peatland type than soil depth. Actinomycetota, Proteobacteria, Firmicutes, Chloroflexota, Acidobacteriota, and Bacteroidota were the predominant bacterial phyla across all soil samples. Moreover, there were no significant differences in the functional pathways between the three peatlands at each depth, except for amino acid metabolism, membrane transport, cell motility, and signal transduction. Redundancy analysis (RDA) revealed that pH and soil water content were the primary environmental factors influencing the bacterial community structure. Therefore, this study is crucial to accurately forecast potential changes in peatland ecosystems and improve our understanding of the role of peat microbes as carbon pumps in the process of permafrost degradation.

DCUN1D3 (DCN1, defective in cullin neddylation 1, domain containing 3) was found during the process of high throughput screening of novel human genes associated with serum response element (SRE) pathway activation. The DCUN1D3 gene is highly conserved among vertebrates. Human DCUN1D3 complementary DNA (cDNA) encodes 304 amino acids with an apparent molecular mass of 34 kDa. However, there has been no report about the function of DCUN1D3. This study detected that DCUN1D3 was broadly expressed in several tumor tissues and cultured cell lines; however, UVC irradiation of different doses significantly increased DCUN1D3 expression level in these cancer cell lines. Over‐expression of the DCUN1D3 inhibits cell growth in HeLa. When the DCUN1D3 gene was silenced by siRNA in UVC‐treated HeLa, the cell cycle in S phase was remarkably blocked; furthermore, the UVC‐induced cell death was inhibited. In addition, DCUN1D3 localized mainly in the cytoplasm and perinuclear, but after UVC treatment, the DCUN1D3 gradually entered the nucleus. All the results above indicate that DCUN1D3 is a novel UVC‐response gene involved in cell cycle regulation and cell survival. (Cancer Sci 2008; 99: 2128–2135)

Climate warming can affect freeze–thaw cycle (FTCs) patterns in northern high-latitude regions and may affect permafrost carbon emissions. The response of carbon release and microbial communities to FTCs has not been well characterized. Here, we conducted laboratory incubation experiments to investigate the relationships among carbon emissions, bacterial community, and soil variables in a permafrost-affected forest–wetland ecotone in Northeast China. The emission rates of CO₂ and CH₄ increased during the FTCs. FTC amplitude, FTC frequency, and patch type had significant effects on carbon emissions. FTCs increased the contents of soil DOC, NH₄⁺-N, and NO₃⁻-N but reduced bacterial alpha diversity. CO₂ emissions were mainly affected by bacterial alpha diversity and composition, and the inorganic nitrogen content was the important factor affecting CH₄ emissions. Our findings indicated that FTCs could significantly regulate CO₂ and CH₄ emissions by reducing bacterial community diversity and increasing the concentration of available soil substrates. Our findings shed new light on the microorganism-substrate mechanisms regulating the response patterns of the soil carbon cycle to FTCs in permafrost regions.