Mixotrophs combine photosynthesis with phagotrophy to cover their demands in energy and essential nutrients. This gives them a competitive advantage under oligotropihc conditions, where nutrients and bacteria concentrations are low. As the advantage for the mixotroph depends on light, the competition between mixo- and heterotrophic bacterivores should be regulated by light. To test this hypothesis, we incubated natural plankton from the ultra-oligotrophic Eastern Mediterranean in a set of mesocosms maintained at 4 light levels spanning a 10-fold light gradient. Picoplankton (heterotrophic bacteria (HB), pico-sized cyanobacteria, and small-sized flagellates) showed the fastest and most marked response to light, with pronounced predator-prey cycles, in the high-light treatments. Albeit cell specific activity of heterotrophic bacteria was constant across the light gradient, bacterial abundances exhibited an inverse relationship with light. This pattern was explained by light-induced top-down control of HB by bacterivorous phototrophic eukaryotes (PE), which was evidenced by a significant inverse relationship between HB net growth rate and PE abundances. Our results show that light mediates the impact of mixotrophic bacterivores. As mixo- and heterotrophs differ in the way they remineralize nutrients, these results have far-reaching implications for how nutrient cycling is affected by light.

Integrated multitrophic aquaculture (IMTA) is an innovative mariculture methodology that reduces the environmental footprint and increases the profitability of the farm. It combines the cultivation of species belonging to different trophic levels, simulating a natural food web. In this study, five Mediterranean species were co-cultured in three operating fish farms in the Aegean (E. Mediterranean) Sea with different trophic conditions. The co-cultivated species were sea bream (Sparus aurata), European sea bass (Dicentrarchus labrax), Mediterranean mussel (Mytilus galloprovincialis), rayed pearl oyster (Pinctada imbricata radiata), and sea cucumber (Holothuria polii). Bream, bass, and mussels were cultivated according to the traditional on-growing methods (fish cages and longlines), whereas the pearl oysters and sea cucumbers were cultivated in baskets designed specifically for oyster farms. To estimate the growth of the co-cultivated species, growth indicators were calculated using length and weight measurements. Furthermore, the growth measurements from co-cultivated species were compared to the respective ones from natural populations. All the species showed high survival rates in the integrated multitrophic aquaculture (IMTA) conditions. Pearl oysters and Mediterranean mussels had positive growth in fish farms with high concentrations of nutrients. Mussel condition index (CI) was 42% in Aquaculture 1 (Aq1) and 33% in Aquaculture 2 (Aq2), compared to 35% in a typical Mediterranean mussel farm. Pearl oysters CI in Aq1 was 53%, in Aq2 56%, in Aquaculture 3 (Aq3) 19%, and in natural populations ranging from 30% to 45%. In contrast, holothurians did not gain weight under the fish cage regime despite the high survival rate. Their final total weight was 17.3 g in Ag1, 8.3 g in Aq2, and 18.3 g in Aq3, but in the natural population, the mean weight was 80 g.