Background: Sect. Tuberculata belongs to Camellia, and its members are characterized by a wrinkled pericarp and united filaments. All the plants in this group, which are endemic to China, are highly valuable for exploring the evolution of Camellia and have great potential for use as an oil source. However, due to the complex and diverse phenotypes of these species and the difficulty of investigating them in the field, their complex evolutionary history and interspecific definitions have remained largely unelucidated. Results: Therefore, we newly sequenced and annotated 12 chloroplast (cp) genomes and retrieved the published cp genome of Camellia anlungensis Chang in sect. Tuberculata. In this study, comparative analysis of the cp genomes of the thirteen sect. Tuberculata species revealed a typical quadripartite structure characterized by a total sequence length ranging from 156,587 bp to 157,068 bp. The cp.genome arrangement is highly conserved and moderately differentiated. A total of 130 to 136 genes specific to the three types were identified by annotation, including protein-coding genes (coding sequences (CDSs)) (87–91), tRNA genes (35–37), and rRNA genes (8). The total observed frequency ranged from 23,045 (C. lipingensis) to 26,557 (C. anlungensis). IR region boundaries were analyzed to show that the ycf1 gene of C. anlungensis is located in the IRb region, while the remaining species are present only in the IRa region. Sequence variation in the SSC region is greater than that in the IR region, and most protein-coding genes have high codon preferences. Comparative analyses revealed six hotspot regions (tRNA-Thr(GGT)-psbD, psbE-petL, ycf15-tRNA-Leu(CAA), ndhF-rpl32, ndhD, and trnL(CAA)-ycf15) in the cp genomes that could serve as potential molecular markers. In addition, the results of phylogenetic tree construction based on the cp genomes showed that the thirteen sect. Tuberculata species formed a monophyletic group and were divided into two evolutionarily independent clades, confirming the independence of the section. Conclusions: In summary, we obtained the cp genomes of thirteen sect. Tuberculata plants and performed the first comparative analysis of this group. These results will help us better characterize the plants in this section, deepen our understanding of their genetic characteristics and phylogenetic relationships, and lay the theoretical foundation for their accurate classification, elucidation of their evolutionary changes, and rational development and utilization of this section in the future.

Background: Section Camellia is the most diverse group in the genus Camellia L., and this group of plants has a long history of cultivation in China as popular ornamental flowers and oil plants. Sect. Camellia plants present diverse morphological variations and complexity among species, resulting in uncertainty in the classification of species, which has resulted in a degree of inconvenience and confusion in the use of plant resources and research. Methods: Here, We sequenced and assembled the chloroplast genomes of 6 sect. Camellia and performed comparative chloroplast genome analysis and phylogenetic studies combined with 15 existing sect. Camellia plants. Results: The chloroplast genome of 21 species in sect. Camellia species were quadripartite with length of 156,587–157,068 bp base pairs (bp), and a highly conserved and moderately differentiated chloroplast genome arrangement. The 21 sect. Camellia chloroplast genomes were similar to those of angiosperms, with high consistency in gene number, gene content and gene structure. After the annotation process, we identified a total of 132 genes, specifically 87 sequences coding for proteins (CDS), 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. The ycf1 gene in 21 species of the sect. Camellia was present only in the small single-copy/inverted repeat of a (SSC/IRa) region. Sequence variation was greater in the large single-copy (LSC) region than in the IR region, and the majority of the protein-coding genes presented high codon preferences. The chloroplast genomes of 21 plant species exhibit relatively conserved SC (single copy region)/IR (inverted repeat region) boundaries. We detected a total of 2975 single sequence repeats (SSRs) as well as 833 dispersed nuclear elements (INEs). Among these SSRs, A/T repeats and AT/AT repeats dominated, while among INEs, forward repeats and palindromic repeats predominated. Codon usage frequencies were largely similar, with 30 high-frequency codons detected. Comparative analysis revealed five hotspot regions (rps16, psaJ, rpl33, rps8, and rpl16) and two gene intervals (atpH-atpI and petD-rpoA) in the cp genome, which can be used as potential molecular markers. In addition, the phylogenetic tree constructed from the chloroplast genome revealed that these 21 species and Camellia oleifera aggregated into a single branch, which was further subdivided into two evolutionarily independent sub-branches. Conclusions: It was confirmed that sect. Camellia and C. oleifera Abel are closely related in Camellia genus. These findings will enhance our knowledge of the sect. Camellia of plants, deepen our understanding of their genetic characteristics and phylogenetic pathways, and provide strong support for the scientific development and rational utilization of the plant resources of the sect. Camellia.

Camellia luteoflora Y.K. Li ex Hung T. Chang & F.A. Zeng belongs to the Camellia L. genus (Theaceae Mirb.). As an endemic, rare, and critically endangered species in China, it holds significant ornamental and economic value, garnering global attention due to its ecological rarity. Despite its conservation importance, genomic investigations on this species remain limited, particularly in organelle genomics, hindering progress in phylogenetic classification and population identification. In this study, we employed high-throughput sequencing to assemble the first complete mitochondrial genome of C. luteoflora and reannotated its chloroplast genome. Through integrated bioinformatics analyses, we systematically characterized the mitochondrial genome’s structural organization, gene content, interorganellar DNA transfer, sequence variation, and evolutionary relationships.Key findings revealed a circular mitochondrial genome spanning 587,847 bp with a GC content of 44.63%. The genome harbors70 unique functional genes, including 40 protein-coding genes (PCGs), 27 tRNA genes, and 3 rRNA genes. Notably, 9 PCGs contained 22 intronic regions. Codon usage analysis demonstrated a pronounced A/U bias in synonymous codon selection. Structural features included 506 dispersed repeats and 240 simple sequence repeats. Comparative genomics identified 19 chloroplast-derived transfer events, contributing 29,534 bp (3.77% of total mitochondrial DNA). RNA editing prediction revealed 539 C-to-T conversion events across PCGs. Phylogenetic reconstruction using mitochondrial PCGs positioned C. luteoflora in closest evolutionary proximity to Camellia sinensis var. sinensis. Selection pressure analysis (Ka/Ks ratios < 1 for 11 PCGs) and nucleotide diversity assessment (Pi values: 0–0.00711) indicated strong purifying selection and low sequence divergence.This study provides the first comprehensive mitochondrial genomic resource for C. luteoflora, offering critical insights for germplasm conservation, comparative organelle genomics, phylogenetic resolution, and evolutionary adaptation studies in Camellia species.

Background: The systematic status of sect. Tuberculata and its taxonomy have recently attracted considerable attention. However, the different bases for defining the characteristics of sect. Tuberculata has led to many disagreements among the plants in this group. Camellia neriifolia and Camellia ilicifolia have been the subject of taxonomic controversy and have been treated as different species or varieties of the same species. Therefore, it is important to use multiple methods, i.e., integrative taxonomy, to determine the taxonomic status of C. neriifolia and C. ilicifolia. This is the first study to systematically explore the taxonomic position of these two plants on the basis of Morphology, Anatomy, Palynology and Molecular Systematics. Results: Extensive specimen reviews and field surveys showed that many differences exist in C. neriifolia and C. ilicifolia, such as the number of trunk (heavily debarked vs. slightly peeling), leaf type (smooth thin leathery, shiny vs. smooth leathery, obscure or slightly shiny), leaf margin (entire vs. serrate), flower type (subsessile vs. sessile), number of styles (3–4 vs. 3), and sepal (ovate vs. round). Moreover, C. neriifolia has a more distinctive faint yellow flower color, and trunk molting was more severe in C. neriifolia than that in C. ilicifolia. In addition, micromorphological analysis of the leaf epidermis showed that the two species differed in the anticlinal wall, stomatal apparatus, and stomatal cluster, and pollen morphology analyses based on pollen size, germination furrow, and polar and equatorial axes showed that they are both distinct from each other. The results of the phylogenetic tree constructed based on the whole chloroplast genome, protein-coding genes, and ITS2 showed that both C. ilicifolia and C. neriifolia were clustered in different branches and gained high support. Conclusions: The results combine morphology, anatomy, palynology, and molecular systematics to treat both C. neriifolia and C. ilicifolia as separate species in the sect. Tuberculata, and the species names continue to be used as they were previously. In conclusion, clarifying the taxonomic status of C. neriifolia and C. ilicifolia deepens our understanding of the systematic classification of sect. Tuberculata.

Camellia tachangensis F. C. Zhang is an endemic Camellia species of the junction of Yunnan, Guizhou and Guangxi Provinces in China. It is characterized by a primitive five-chambered ovary morphology and serves as the botanical source of the renowned “Pu’an Red Tea”. Unfortunately, the populations of the species have declined due to the destruction of their habitats by human activities. The lack of mitochondrial genomic resources has hindered research into molecular breeding and phylogenetic evolution of C. tachangensis. In this study, we had sequenced, assembled, and annotated the mitochondrial genome of C. tachangensis to reveal its genetic characteristics and phylogenetic relation with other Camellia species. The assembly result indicated that the mitochondrial genome sequence of C. tachangensis was 746,931 bp (GC content = 45.86%). It consisted of one multibranched sequence (Chr1) and one circular sequence (Chr2), with Chr1 capable of producing 7 substructures. The comparative analysis of the mitochondrial and chloroplast DNA of C. tachangensis revealed 23 pairs of chloroplast homologous fragments, with 10 fully preserved tRNA genes within them. Interspecies comparison of Ka/Ks ratios revealed that mutations in mitochondrial protein-coding genes (PCGs) of C. tachangensis were predominantly shaped by purifying selection throughout its evolution (Ka/Ks < 1). The mitochondrial CDS-based phylogenetic tree indicated that within the Camellia lineage, C. tachangensis was phylogenetically independent of the species of sections Oleifera, Camellia, Heterogenea, and Chrysantha. However, it also did not support the clustering of C. tachangensis with certain variants of C. sinensis, due to the extremely low support (BS = 22, PP = 0.41). Meanwhile, the chloroplast PCG-based phylogenetic analysis revealed that C. tachangensis formed a strongly supported basal clade (BS = 100, PP = 1.00), alongside C. makuanica (NC_087766), C. taliensis (NC_022264), and C. gymnogyna (NC_039626). Our study deciphered the mitochondrial genome and its multibranched structure of C. tachangensis. These findings not only enhanced our comprehension of the complexity and diversity of mitochondrial genome structures in Camellia species, but also established a foundational genetic data framework for future research on molecular breeding programs and phylogenetic relationship involving C. tachangensis and its related species.

A new Asteraceae species, Ixeridium malingheense Z.Li & Q.Xu sp. nov., from Xingyi City, south-western Guizhou, China, is described and illustrated based on morphological and molecular analyses. Compared with the other species of the genus, the species is most similar to I. yunnanense in a small stature, linear-lanceolate stem leaves, and short phyllaries. However, it is easily distinguished from the latter by its smaller basal leaves 18–34 × 5–13 mm (vs. 10–25 × 5 mm), spatulate or long-spatulate (vs. elliptic, lanceolate, or oblanceolate), petioles ca. 30–55 mm (vs. 10 mm to absent), outer phyllaries 1-seriate (vs. 2-seriate, unequal), achenes smaller 2.0–3.0 × 0.5–1.0 mm (vs. 3.2 × 0.6 mm), and a flowering period of March to May (vs. June). Molecular phylogenetic analyses based on nuclear ITS sequence data determined the systematic position of I. malingheense in Ixeridium.