Background: In this study, the effect of pure caffeine was established against Candida albicans (C. albicans) using different microbiological techniques. Methods: Broth microdilution and colony forming units (CFUs) assays were used to detect the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The Live/Dead fluorescent dyes were implemented to determine the yeast viability. Polymethyl methacrylate acrylic resin (PMMA) discs were prepared to evaluate caffeine’s effects against adherent C. albicans using microplate reader, CFUs, and scanning electron microscope (SEM). Results: caffeine’s MIC was detected around 30 mg/mL, while the MFC was considered at 60 mg/mL. In an agar-well diffusion test, the inhibition zones were wider in caffeine groups. The Live/Dead viability test verified caffeine’s antifungal effects. The optical density of the adherent C. albicans on PMMA discs were lower at 620 nm or 410 nm in caffeine groups. CFU count was also reduced by caffeine treatments. SEM revealed the lower adherent C. albicans count in caffeine groups. The effect of caffeine was dose-dependent at which the 60 mg/mL dose demonstrated the most prominent effect. Conclusion: The study reinforced caffeine’s antifungal and antibiofilm properties and suggested it as an additive, or even an alternative, disinfectant solution for fungal biofilms on denture surfaces.

Background: Fixation of biopsy specimens is a critical step before processing and staining them for histological tissue examination. Formalin is considered the gold standard fixative solution for its attributes. However, it has concerning side effects, such as carcinogenic and potential irritational properties. Owing to its various harmful effects, a safer natural substitute should be explored. In this study, we compared the effectiveness of propolis to 10% formalin and determined its ability as a natural fixative solution. Materials and Methods: Sixty tissue specimens were collected from goats’ tongues and immediately placed in (1) 6.6% propolis, (2) 10% natural buffered formalin (positive control), (3) 6.6% propolis followed by 10% formalin, and (4) 0.9% saline (negative control). Tissue samples were fixed at different time points (12, 24, 48, and 72 h) at room temperature, followed by processing and staining. The quality of the microscopic parameters was blindly assessed by two oral and maxillofacial pathologists using a numerical scoring scale. Scores were statistically analyzed. Results: The fixation of tissue samples placed in 6.6% propolis was statistically significantly better than that of samples placed in 10% formalin and 0.9% saline at different time points. Conclusion: Propolis showed promising fixation properties and can be considered a natural alternative to 10% formalin.

In the field of medicine, diagnosing diseases involves various steps, procedures, and protocols. Histopathological examination remains the gold standard for a definitive and accurate diagnosis. This process relies on an initial step of tissue fixation.

Background The aim of this study was to investigate the knowledge, attitude, and practice (KAP) among scuba divers in Saudi Arabia towards equipment infection control measures, protective guidelines measures and potential post COVID-19 infection complications. Methods A cross-sectional study using a pre-validated questionnaire was conducted. The questionnaire consisted of 35 close-ended questions, which covered the scuba divers’ profile and experience, the KAP of COVID-19 precautionary measures during diving activities as well as the demographic data and background of the scuba divers. KAP scores were subjected to non-parametric tests of statistical significance (Mann Whitney and Kruskal Wallis test). Statistical significance was set at p < 0.05. Results Most of the 461 participants’ answers were in agreeance on the proper KAP of infection control during the COVID-19 pandemic. There was a statistically significant difference in attitude between all the demographic and professional variables (p0.05). In addition, there was a statistically significant practice difference among age groups, education levels, and employee status in the diving center (p<0.05). Conclusions Our findings showed that scuba divers presented a good level of KAP of infection control measures against the spread of the coronavirus disease. Local officials and diving organizations need to continue their efforts in combating and control the spread of this pandemic.

Background The aim of this study was to investigate the knowledge, attitude, and practice (KAP) among scuba divers in Saudi Arabia towards equipment infection control measures, protective guidelines measures and potential post COVID-19 infection complications. Methods A cross-sectional study using a pre-validated questionnaire was conducted. The questionnaire consisted of 35 close-ended questions, which covered the scuba divers’ profile and experience, the KAP of COVID-19 precautionary measures during diving activities as well as the demographic data and background of the scuba divers. KAP scores were subjected to non-parametric tests of statistical significance (Mann Whitney and Kruskal Wallis test). Statistical significance was set at p < 0.05. Results Most of the 461 participants’ answers were in agreeance on the proper KAP of infection control during the COVID-19 pandemic. There was a statistically significant difference in attitude between all the demographic and professional variables (p0.05). In addition, there was a statistically significant practice difference among age groups, education levels, and employee status in the diving center (p<0.05). Conclusions Our findings showed that scuba divers presented a good level of KAP of infection control measures against the spread of the coronavirus disease. Local officials and diving organizations need to continue their efforts in combating and control the spread of this pandemic.

Background: The aim of this study was to investigate the knowledge, attitude, and practice (KAP) among scuba divers in Saudi Arabia towards equipment infection control measures, protective guidelines measures and potential post COVID-19 infection complications. Methods: A cross-sectional study using a pre-validated questionnaire was conducted. The questionnaire consisted of 35 close-ended questions, which covered the scuba divers’ profile and experience, the KAP of COVID-19 precautionary measures during diving activities as well as the demographic data and background of the scuba divers. KAP scores were subjected to non-parametric tests of statistical significance (Mann Whitney and Kruskal Wallis test). Statistical significance was set at p < 0.05. Results: Most of the 461 participants’ answers were in agreeance on the proper KAP of infection control during the COVID-19 pandemic. There was a statistically significant difference in attitude between all the demographic and professional variables (p0.05). In addition, there was a statistically significant practice difference among age groups, education levels, and employee status in the diving center (p<0.05). Conclusions: Our findings showed that scuba divers presented a good level of KAP of infection control measures against the spread of the coronavirus disease. Local officials and diving organizations need to continue their efforts in combating and control the spread of this pandemic.