Several factors influence fruit set and yield in strawberry production. Bees play an important role in pollinating strawberry flowers; however, bee populations are decreasing due to air pollution and high usage of pesticides. Boron (B) can increase fruit set by improving pollen viability, germination, and pollen tube growth. This study aimed to assess how the application of honey bees and B fertilizer affects yields and marketable fruit quality of Fortuna strawberry cultivar. The experiment used two strawberry growing tunnels. One was open, thus allowing bee activity, and the other was closed, which meant that bees could not access the strawberry flowers. Each tunnel had different B applications. The presence of bees increased the total fruit yield per plant by 54% compared to the results of plants that were not pollinated by bees. Plants treated with bees yielded bigger fruit than those without bee treatment, which is likely a result of more achenes having been fertilized. Fruit formation, however, did not depend directly on pollinator activity. Both in the presence and absence of bees, applying B to the soil increased yields and decreased misshapen fruit rates. Applying B to the soil was more effective than B foliar applications. As a result of this study, it is demonstrated that strawberry plants perform at a higher rate with the application of B and the presence of bee activity.