This paper explores the mechanisms of biochar that facilitate direct interspecies electron transfer (DIET) among syntrophic microorganisms leading to improved anaerobic digestion. Properties such as specific surface area (SSA), cation exchange capacity (CEC), presence of functional groups (FG), and electrical conductivity (EC) were found favorable for increased methane production, reduction of lag phase, and adsorption of inhibitors. It is revealed that these properties can be modified and are greatly affected by the synthesizing temperature, biomass types, and residence time. Additionally, suitable biochar concentration has to be observed since dosage beyond the optimal range can create inhibitions. High organic loading rate (OLR), pH shocks, quick accumulation and relatively low degradation of VFAs, and the presence of heavy metals and toxins are the major inhibitors identified. Summaries of microbial community analysis show fermentative bacteria and methanogens that are known to participate in DIET. These are Methanosaeta, Methanobacterium, Methanospirillum, and Methanosarcina for the archaeal community; whereas, Firmicutes, Proteobacteria, Synergistetes, Spirochetes, and Bacteroidetes are relatively for bacterial analyses. However, the number of defined cocultures promoting DIET is very limited, and there is still a large percentage of unknown bacteria that are believed to support DIET. Moreover, the instantaneous growth of participating microorganisms has to be validated throughout the process. Graphical abstract

This study is motivated to increase the value of cocoa pod husk (CPH), which is currently considered as waste in the cocoa farming, by converting it to activated carbon (AC). The main goal is to comparatively evaluate the impact of H₃PO₄ and KOH as chemical-activating agents on the resulting textural properties of the obtained carbon from CPH. Furthermore, the effects of the impregnation ratio and precursor’s particle size were also examined. In all cases considered, H₃PO₄-activated CPH obtained a higher yield and more desirable properties over KOH-activated CPH. Characterization of the physisorptive properties such as Brunauer–Emmett–Teller surface area (S_BET), total pore volume (V_total), and average pore diameter (D_p) also suggested that H₃PO₄ is the better activating agent. The highest S_BET obtained was 1237.47 m²/g from the 2.00:1.00 impregnation ratio. V_total (1.11 cm³/g) was also found to be the highest at this condition. Further analyses on the D_p and pore size distribution revealed that AC obtained from H₃PO₄ was mesoporous. Proposed porosity development on both activating agents suggest that KOH is a more reactive activating agent for CPH than H₃PO₄, as evidenced by severe material loss and low structural integrity.

Three coarse herbaceous energy plants—such as Miscanthus (Miscanthus sinensis), sida (Sida hermaphrodita Rusby) and cup plant (Silphium perfoliatum L.)—were grown and investigated in the experimental fields of Vytautas Magnus University Agriculture Academy, and the technical means of plant processing and utilization for solid biofuel were investigated. The physical–mechanical properties and quality indicators (moisture content, biometrical properties, density, and resistance to compression) of coarse stem herbaceous plants milled and compressed into 6 mm diameter pellets were investigated. The moisture content of the tested pellets was sufficiently low and ranged from 8.7% to 9.6%. The highest density was that of sida pellets (1072.3 ± 43.4 kg m⁻³ DM), and the lowest density was that of Miscanthus pellets (713.5 ± 67.1 kg m⁻³ DM). In order to evaluate the influence of moisture content on the properties of biofuel pressed into pellets, the density and the destructive compressive force of the different-moisture pellets were investigated and their change in the range of 5–15% pellet moisture content was evaluated. Criterion k was calculated to determine the effect of moisture on the pellet quality indicators (density, destructive compressive force, and lower heating value), and the following results were obtained: the highest influence of moisture on density was observed in sida (k = 34.280), on destructive compressive force in Miscanthus (k = 14.5), and on the lower heating value, also in Miscanthus (k = 0.198). After a comprehensive investigation and evaluation of these properties, an empirical model suitable for practical use was developed and prepared. Emissions of harmful gases, such as carbon monoxide, carbon dioxide, and nitrogen oxides, were determined when various coarse stem herbaceous energy plants were burned. The determined emissions of harmful gases into the environment did not exceed the permissible values.

Hydrothermal carbonization (HTC) increasingly appears as an eco-friendly method for managing food waste (FW). In this work, a combination of FW was subjected to HTC, and products were critically evaluated. This involved a lab-scale pressure reactor and optimization of HTC conditions: temperature (220-340 °C) and residence time (90-260 min) via central composite design type of response surface methodology (CCD-RSM). Results showed varying temperatures and residence time to impact the hydrochar (HC) and hydrothermal carbonization aqueous phase (HTC-AP) properties. Although HC produced through HTC exhibited lower ash content (55 %) with respect to the raw FW, the heating value of HC ranged from 19.2 to 32.5 MJ/kg. Temperature primarily influenced FW conversion, affecting carbonaceous properties. Saturated fatty acids (SFA) were found to be predominant in the HTC-AP under all tested operating conditions (77.3, 48.4, and 37.1 wt% for HTC at 340, 280, and 220 °C in 180 min, respectively). Total phosphorus recovery in HC and HTC-AP respectively peaked at 340 °C and 220 °C in 180 min. The study concludes that HTC holds promise for energy-dense biofuel production, nutrient recovery, and fostering a circular economy.

The influence of the inoculum‐to‐substrate ratio (ISR) on anaerobic digestion (AD) of biomass in terms of methane yield and microbial community, was explored in this paper. The level of ISR can affect the AD performance in several ways. At extremely low ISR, volatile fatty acids (VFAs) accumuate, while inhibition occur at higher level of ISR. An ISR ranging from 1.0–2.0 was found optimal resulting in higher methane yield, organic matter removal and VFA degradation. Furthermore, a high ISR (2.0–4.0) is favourable to methanogenesis, while a lower ISR (<1.0) is prone to irreversible acidification. The range of ISR can shift the methanogenic pathway of AD to favour an acetoclastic or hydrogenotrophic response, indicated by the enriched group of microorganisms. The genus Methanosaeta (acetoclastic) and Methanobacterium (hydrogenotrophic) are the most enriched methanogens across all ISRs, while Firmicutes , Bacteroidetes , Proteobacteria and Spirochaetae are dominant in the bacterial community. Additionally, the interplay of substrate biodegradability and ISR potentially affects AD performance. Finally, novel equations are developed and proposed for characterizing the quantity of inoculum and substrate.