Antimicrobial resistance (AMR) of brucellosis major causative bacteria Brucella abortus and Brucella melitensis is complicating human treatment strategies in the Mediterranean basin, where the disease was first reported and is still endemic. The current meta‐analysis examines the prevalence and patterns of AMR in 119 Brucella abortus and 1344 Brucella melitensis isolates across Mediterranean countries, highlighting significant geographical disparities in resistance data. The E‐test and disc diffusion were mostly used for measuring antimicrobial susceptibility, which was validated by the CLSI guidelines of Haemophilus spp. or bacteria of bioterrorism. Genotypic detection of resistance was conducted in a few studies. Despite the documented burden of brucellosis, studies on AMR remain scarce, particularly in North Africa, the Middle East, and several European Mediterranean nations. Comparative phenotypic–genotypic resistograms were reported in only a few studies, yet they are essential for understanding the mechanisms of AMR in this serious zoonotic pathogen. The analysis revealed a high overall AMR proportion (32%, 95% confidence interval [CI]: 16%–51%) with considerable heterogeneity ( I ² = 97%, p < 0.01). Notable differences in resistance were observed between regions, with African Mediterranean countries exhibiting the highest resistance rates (71%, 95% CI: 44%–94%) and European Mediterranean countries the lowest (9%, 95% CI: 0%–42%). Eastern Mediterranean countries exhibited higher resistance rates than their western counterparts ( p = 0.11). Brucella abortus showed higher resistance (63%, 95% CI: 25%–95%) than Brucella melitensis (24%, 95% CI: 8%–43%). Isolates of bovine origin displayed the highest percentage of resistance (89%, 95% CI: 69%–100%) compared to isolates of other origins. Resistance to rifampicin and trimethoprim–sulfamethoxazole was generally low, but macrolide resistance, especially to azithromycin, was notably higher in African countries ( p < 0.01). This study underscores the need for standardized AMR surveillance based on Brucella‐specific validation criteria, which are lacking, improved testing methodologies, and region‐specific interventions to address AMR in brucellosis, particularly in livestock, where resistance is more prevalent. The findings highlight the importance of targeted antibiotic stewardship and monitoring to mitigate the spread of resistant Brucella strains and protect public health.

This study aimed to investigate a sheeppox outbreak in a highly susceptible naive sheep population in Kharsit village, Gharbia Governorate, Egypt. Moreover, to compare commercial sheeppox vaccines, the Romanian strain and RM-65 vaccines, as emergency vaccination against sheeppox under field conditions. In December 2018, a sheeppox outbreak occurred in a flock of 65 sheep upon the purchase of an apparently healthy ewe from outside the village. This ewe showed a systemic disease with cutaneous lesions after a few days, thereafter more cases began to appear. Cutaneous lesions in other sheep in the flock in the form of macules, papules, and scabs were common in wool-less areas of the body, in addition to fever and respiratory disorders. Postmortem findings revealed the congestion of visceral organs with apparent gross pathology of the lung. Biopsies of cutaneous lesions and visceral organs were collected, and sheeppox was identified by histopathology and transmission electron microscopy, which showed the existence of sheeppox cells and intracytoplasmic brick-shape sheeppox virions. The Romanian strain and RM-65 vaccines were used for the emergency vaccination for two different groups of animals and the third group was left as a control group. Serum samples were collected before vaccination as well as 21 days post-vaccination, and serum protein fractionation analysis was performed for all groups. The outbreak ended after 2.5 months, the cumulative incidence was 66.2%, and the overall case fatality was 51.1%. There was significantly higher protection against sheeppox infection and mortalities among RM-65 vaccine immunized group compared to Romanian strain vaccine-immunized animals at p < 0.05. RM-65-vaccinated animals did not show sheeppox cases or mortalities, compared to Romanian strain-vaccinated animals, which had mild pox signs in 78% of animals and case fatality of 35.7%. The serum protein analysis also indicated the superior performance of the RM-65 vaccine; it increased the level of α1-globulin and β-globulin compared to the Romanian strain, which increased the level of β-globulin only. The current study shows a better performance of the tested RM-65 than the Romanian strain vaccine for emergency vaccination against sheeppox under field conditions. These findings point to the validity of emergency vaccination against sheeppox and the importance of the comparative field evaluation of vaccines; however, wide-scale studies are required for further evaluation. Future investigation of whether the Romanian strain itself or vaccine-production-related issues are responsible for these findings is required.

This study was conducted on dairy farms in Gharbia governorate, Egypt. From January to December 2018 .The study included 240 dairy farms and 3775 serum sample were collected for serological tests.25 farms (10.42) were positive for brucellosis,132 serum sample were positive by serological test divided according to production stage of animals as 121/3000(4.033%), 8/600 (1.33%) and 2/175 (1.142%) in cows ,heifers and bulls respectively. The result of culturing and confirmation by PCR indicated that Brucella melitensis biovar 3 is the dominant strain in dairy farms in Gharbia governorate. The spatial distribution of positive cases indicated that districts of Samnood, Kfr- Elzyat and Basion had the higher percent of positive cases as 6.35%, 4.67% and 3.57% respectively, however districts of El-Santa, Zefta and El-Mahla El-Kobra had the lowest percent as 1.49%, 2.3% and 2.89% respectively. The obtained result proved that, brucellosis is endemic in Gharbia governorate and good control program should be conducted to eradicate the disease.

The current study was applied from January to December 2019 to determine and identify the prevalence rateof bovine brucellosis and its correlated risk factors in dairy herds in Nile delta, Egypt. The study populations comprised of 300 dairy farms which including 4000 dairy cattle. Estimated results showed that, the prevalence of brucellosis in dairy cattle was 6.05 % that depended on the result of CFT. The univariate statistical analysis revealed that positive cases of brucellosis was clearly higher in cattle housed under the intensive management system, and animals in the extensive management system had lower prevalence (P 0.05). Significant increasing of positive cases was parallel with the increasing of the size of herd (P < 0.05). Sero-positivity to brucellosis was significantly correlated with history of abortions or stillbirths. The results estimated that brucellosis is endemic and widely distributed disease in Nile delta, Egypt.