In this work, we recall some definitions on fractional calculus with discrete-time. Then, we introduce a discrete-time Hopfield neural network (D.T.H.N.N) with non-commensurate fractional variable-order (V.O) for three neurons. After that, phase-plot portraits, bifurcation and Lyapunov exponents diagrams are employed to verify that the proposed discrete time Hopfield neural network with non-commensurate fractional variable order has chaotic behavior. Furthermore, we use the 0-1 test and $C_0$ complexity algorithm to confirm and prove the results obtained about the presence of chaos. Finally, simulations are carried out in Matlab to illustrate the results.