SUMMARY. This study evaluated the influence of inoculation of different nutrients into eggs of 18-d-old broiler embryos. On d 18 of incubation, before transfer
1 March 2018 in Journal of Applied Poultry Research
Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry.
1 March 2018 in Journal of Applied Poultry Research
Um experimento foi conduzido para se determinar o valor energético do óleo de soja refinado, do óleo de canola refinado, do óleo de girassol refinado, do óleo de frango, do óleo de peixe e da banha suína para frangos de corte. Foram utilizados 168 frangos de corte com 22 dias de idade, distribuídos em um delineamento inteiramente casualizado, com sete tratamentos (seis fontes lipídicas e uma ração-referência) e quatro repetições de seis frangos por unidade experimental. O experimento teve duração de oito dias, sendo a coleta de excretas realizada nos últimos cinco dias. As fontes lipídicas testadas substituíram a dieta basal em 20% na matéria natural. Os valores de energia metabolizável aparente corrigidos para retenção de nitrogênio na matéria natural foram: 9.201 kcal/kg para o óleo de soja; 8.129 kcal/kg para o óleo canola; 9.561 kcal/kg para o óleo de girassol; 8.251 kcal/kg para o óleo de frango; 8.715 kcal/kg para o óleo de peixe e 8.366 kcal/kg para a banha suína.
1 December 2011 in Revista Brasileira de Zootecnia
The objective of the present study was to evaluate the influence of a probiotic product (composition: Lactobacillus acidophillus (3.5 × 10(11) CFU), Streptcoccus faecium (3.5 × 10(11) CFU) and Bifidobacterium bifidum (3.5 × 10(11) CFU)) on broiler performance. A total of 1200 one-day-old broilers were reared until 42 days of age, and distributed in a completely randomized experimental design with 3 treatments (antibiotic, probiotic and control) with 10 replicates of 40 birds each. Weight gain, feed intake, feed conversion ratio and mortality were evaluated. Concerning weight gain, in the periods of 0-7 and 0-14 days of age, the group fed the antibiotic product presented higher values as compared with the other treatments. However, in the periods of 0-21, 0-28 and 0-35 days of age, birds fed the antibiotic presented higher weight gain only in relation to the control group. Feed intake differences were detected only in the initial period of 0-7 days of age, with the group fed the antibiotic product presenting higher feed intake as compared with that fed the probiotic product, although these groups were not different from the control group. No statistical difference was detected in feed conversion ratio among treatments in any of the evaluated age intervals. Mortality was different only in the period of 0-14 days of age, which was higher in the control group as compared with that of the birds fed the probiotic product, but it was not different from the group receiving the antibiotic. Treatment with probiotic product containing Lactobacillus acidophillus, Streptococcus faecium and Bifidobacterium bifidum does not affect broiler performance