A change of loading conditions in the knee causes changes in the subchondral bone and may be a cause of osteoarthritis (OA). However, quantification of trabecular architecture in vivo is difficult due to the limiting spatial resolution of the imaging equipment; one approach is the use of texture parameters. In previous studies, we have used digital models to simulate changes of subchondral bone architecture under OA progression. One major result was that, using computed tomography (CT) images, subchondral bone mineral density (BMD) in combination with anisotropy and global homogeneity could characterize this progression. The primary goal of this study was a comparison of BMD, entropy, anisotropy, variogram slope, and local and global inhomogeneity measurements between high-resolution peripheral quantitative CT (HR-pQCT) and CT using human cadaveric knees. The secondary goal was the verification of the spatial resolution dependence of texture parameters observed in the earlier simulations, two important prerequisites for the interpretation of in vivo measurements in OA patients. The applicability of texture analysis to characterize bone architecture in clinical CT examinations was investigated and compared to results obtained from HR-pQCT. Fifty-seven human knee cadavers (OA status unknown) were examined with both imaging modalities. Three-dimensional (3D) segmentation and registration processes, together with automatic positioning of 3D analysis volumes of interest (VOIs), ensured the measurement of BMD and texture parameters at the same anatomical locations in CT and HR-pQCT datasets. According to the calculation of dice ratios (>0.978), the accuracy of VOI locations between methods was excellent. Entropy, anisotropy, and global inhomogeneity showed significant and high linear correlation between both methods (0.68 < R 2 < 1.00). The resolution dependence of these parameters simulated earlier was confirmed by the in vitro measurements. The high correlation of HR-pQCT- and CT-based measurements of entropy, global inhomogeneity, and anisotropy suggests interchangeability between devices regarding the quantification of texture. The agreement of the experimentally determined resolution dependence of global inhomogeneity and anisotropy with earlier simulations is an important milestone towards their use to quantify subchondral bone structure. However, an in vivo study is still required to establish their clinical relevance.

To use standard radiographs to determine which combination of co-occurrence textural parameters, geometric measurements, and cortical thickness measurements from femur radiographs provided the best estimate of femoral failure load and to compare these with total hip dual-energy x-ray absorptiometry bone mineral density (BMD) evaluation. Digital radiographs of 40 pairs of excised femurs (24 women, 16 men; mean age, 82 years + or - 12 [standard deviation]) were obtained. Regions of interest in the femoral neck, greater trochanter, intertrochanteric area, and femoral head were then selected. Three textural parameters derived from a co-occurrence matrix were estimated with imaging software. Neck-shaft angle, femoral neck axis length, calcar femorale thickness, and internal and external femoral shaft thickness were assessed. The femurs were randomly allocated to single-stance (femoral neck fracture) or side-impact (intertrochanteric fracture) configurations for failure load measurement. Textural parameters correlated significantly with site-matched BMD. Stepwise regression analysis was performed, and total hip BMD explained 73% and 78% of the failure load in single-stance and side-impact configurations, respectively. Combining internal femoral shaft thickness with one or two textural parameters explained 72%-79% of failure load variance in the single-stance configuration and 63%-76% of failure load variance in the side-impact configuration. In these excised femurs, combining textural parameters with cortical thickness measurements had a performance comparable to that of BMD alone in the explanation of femoral failure load.

An in vitro approach enabled to investigate the relationship between bread crumb porous micro structure and kinetics of hydration.