In view of the problem of sustainable restoration of vegetation in alpine mining areas, vegetation communities and physical and chemical properties of soil under different artificial restoration measures (i.e., grass monoculture, sowing quantity and topsoil replacement) were investigated for five consecutive years (2016–2020) in the sloped eastern area of a northern slag mound in the Jiangcang mining area of the Muli coalfield in Qinghai, China. The results showed that the vegetation characteristics of different sowing treatments with different kinds of grass species, such as Elymus nutans, Poa pratensis cv. Qinghai and Poa crymophila cv. Qinghai, were significantly different (p < 0.05). The content of soil available nitrogen and phosphorus was significantly different among different grass species (p < 0.05). Vegetation coverage and ramet density of sowing treatment five were significantly higher than those of a lower sowing quantity (p < 0.05). There was no significant difference in the vegetation characteristics among different grass species treatments five years after the restoration (p > 0.05). There was no significant difference in vegetation height of different soil covering treatments (p > 0.05). In the early stage of restoration, vegetation coverage and ramet density in TR2 (resurfacing soil 10 cm) and TR3 (resurfacing soil 15 cm) were significantly higher than those without treatment. Soil N, P and organic matter under the treatments of TR1 (resurfacing soil 5 cm), TR2 and TR3 were significantly higher than those in CK (p < 0.05) in the early stage of restoration, but there was no significant difference in soil N, P and organic matter after five years of restoration. Overall, the vegetation and soil characteristics showed a trend of increase first and then decrease during the 5-year restoration period under different artificial measures. There were significant differences in the vegetation and soil characteristics among different treatments in the second year of restoration (p < 0.05), but there was no significant difference between the first year and the fifth year of restoration, which indicated that vegetation and soil began to degrade after five years of restoration, and substrate nutrients in the mining waste soil could only support the short-term restoration of vegetation. Therefore, it is necessary to target matrix nutrients in future vegetation restoration in alpine mining areas.

This study aims to enlighten our understanding of the distribution of soil carbon-fixing bacteria (cbbL-harboring bacteria) and their community diversity in differently degraded patches at three altitudes. MiSeq high-throughput sequencing technology was used to analyze the soil carbon-fixing bacteria community diversity of degraded patches and healthy meadow at three altitudes. Redundancy analysis (RDA) and structural equation model (SEM) were used to analyze the correlation and influence path between environmental factors and carbon-fixing bacteria. The results showed that degradation reduced the relative abundance of Proteobacteria from 99.67% to 95.57%. Sulfurifustis, Cupriavidus, and Alkalispirillum were the dominant genera at the three altitudes. Hydrogenophaga and Ectothiorhodospira changed significantly with altitude. RDA results confirmed that available phosphorus (AP) was strongly and positively correlated with Proteobacteria. AP and total nitrogen (TN) were strongly and positively correlated with Hydrogenophaga. Grass coverage and sedge aboveground biomass were strongly and positively correlated with Sulfurifustis and Ectothiorhodospira, respectively. Elevation adversely affected the relative abundance of dominant carbon-fixing bacteria and diversity index by reducing the coverage of grass and soil volumetric moisture content (SVMC) indirectly, and also had a direct positive impact on the Chao1 index (path coefficient = 0.800). Therefore, increasing the content of nitrogen, phosphorus and SVMC and vegetation coverage, especially sedge and grass, will be conducive to the recovery of the diversity of soil carbon-fixing bacteria and improve the soil autotrophic microbial carbon sequestration potential in degraded meadows, especially in high-altitude areas.

This study addresses how to overcome the challenges facing ecological restoration and vegetation recovery in alpine mining areas via indoor controlled pot experiments. The soil layer was recon-structed using a mixture of granular organic fertilizer, sheep manure, and mining slag, and manually sowed with a mixture of five types of grass seeds after potting to assess the impact of dosage of granular organic fertilizer and ratios of sheep manure to mining slag. Results indicate that the dosage of granular organic fertilizer significantly impacted plant characteristics, soil available nitrogen, phosphorus, potassium and pH (P < 0.01), and show significantly positive correlation with the number of dead seedlings. Sheep manure significantly affects soil available phosphorus and bulk density (P < 0.01). The highest grass density and biomass occurred with zero organic fertilizer dosage and a volume ratio of 1:3 between sheep manure and soil (M1S4), indicating that sheep manure effectively enhances seedling emergence. The dosage in the potted experiments is approximately six times higher than that in the alpine mining field. A dosage of granular organic fertilizer exceeding 1.5 kg/m²results in seedling mortality. Therefore, the dosage of granular organic fertilizer in restoration in alpine mining areas should not exceed 9 kg/m².