Numerous studies report on chitosan hydrogels in different forms, such as films, porous structures, nanoparticles, and microspheres, for biomedical applications; however, this study concentrates on their modifications with different crosslinking agents and observes their effects on drug loading and releasing capacities. Linear chitosan, along with chitosans crosslinked with two major crosslinkers, i.e., genipin and disulfide, are used to formulate three different hydrogel systems. The crosslinking process is heavily impacted by temperature and pH conditions. Three different drugs, i.e., thymoquinone, gefitinib, and erlotinib, are loaded to the hydrogels in de-ionized water solutions and released in phosphate-buffered solutions; thus, a total of nine combinations are studied and analyzed for their drug loading and releasing capabilities with ultraviolet–visible (UV–Vis) spectroscopy. This study finds that thymoquinone shows the lowest loading efficacy compared to the two other drugs in all three systems. Gefitinib shows stable loading and releasing regardless of crosslinking system, and the genipin-crosslinked system shows stable loading and releasing with all three drug molecules. These experimental results agree well with the findings of our previously published results conducted with molecular dynamics simulations.

The purposes of this study are to analyze the groundwater quality of the Ogallala Aquifer and evaluate the hydrological characteristics in the southern High Plains region of the Permian Basin, Texas. Levels of chloride, fluoride, nitrate, selenium, pH, and total dissolved solids (TDS) were analyzed for the period 1990–2016. Data concerning a total of 133 wells were collected from the Texas Water Development Board (TWDB), which is an open database provided by the US government. The average levels of contaminants were compared to their respective Maximum Contaminant Levels (MCL) stipulated by the Environmental Protection Agency (EPA). The study area experienced high concentrations of most parameters including chloride, fluoride, nitrate, selenium, and TDS, within the contaminants’ respective MCLs. Borden and Dawson counties experienced the highest overall amounts of groundwater pollutants. Possible sources of each contaminant are discussed, with oil and gas activities, agricultural practices, and other human actions impacting the conditions. This research provides important information about groundwater quality of the Ogallala Aquifer and contributes to understanding the response to development in the Permian Basin, Texas.