Plant height is an important indicator in the ideal plant model and contributes to optimizing yield and lodging resistance. The emergence of a dwarfing phenotype provides an opportunity for plant height improvement. In a previous study, we identified a dwarf mutant Si1 in pumpkin (Cucurbita moschata D.) obtained by ethyl methane sulfonate (EMS) mutagenesis of the inbred line N87. Phenotype identification for Si1 revealed a decrease in cell size and shorter internodes than those of wild type. Genetic analysis revealed that the dwarf mutant trait was controlled by a single recessive gene, CmaSI1. By bulked segregant analysis (BSA) and subsequent fine mapping, we mapped the CmaSI1 locus to a 463 kb region on chromosome 8 that contained 28 annotated genes in the F₂ population. Only one nonsynonymous single nucleotide polymorphism (SNP) in CmoCh08G006170 was obtained according to whole-genome resequencing of the two parents. CmoCh08G006170, a homolog of Arabidopsis gibberellin 3-beta hydroxylase (GA3ox), is a key enzyme in the regulation of bioactive gibberellins (GAs). RNA-seq analysis and qRT-PCR showed that the expression level of CmoCh08G006170 in stems of Si1 was changed compared with that of wild type. The dwarf phenotype could be restored by exogenous GA₃ treatment, suggesting that Si1 is a GA-deficient mutant. The above results demonstrated that CmoCh08G006170 may be the candidate gene controlling the dwarf phenotype. This study provides an important theoretical basis for the genetic regulation of vine length and crop breeding in pumpkin.

In order to scientifically evaluate the germplasm resources of Momordica charantia in southern China, the diversity, correlation and cluster analysis were carried out on the main botanical characters of 56 Momordica charantia varieties, such as melon length, melon transverse diameter, single melon weight, internode length, stem diameter, leaf length and leaf width. The results showed that the variation coefficients of 7 agronomic characters of 56 Momordica charantia varieties ranged from 8.81% to 19.44%, the average variation coefficient was 14.21%, the maximum variation coefficient of single melon weight was 19.44%, and the minimum variation coefficient of melon cross diameter was 8.81%. The correlation analysis showed that there were correlations among the agronomic traits. The positive correlation coefficient between leaf length and leaf width was up to 0.978, and the negative correlation coefficient between single melon weight and internode length was up to 0.451. The 56 varieties were divided into 3 groups by cluster analysis, of which 92.86% of the materials were concentrated in the first and second groups, and there were only 4 materials in the third group. The results can provide a reference for the cultivation, utilization and genetic improvement of Momordica charantia resources in southern China.