Purpose: This study aimed to examine the activities of heart rate variability (HRV) and cortisol secretion as markers of the autonomous nervous system (ANS) and the hypothalamic-pituitary-adrenal axis (HPA) during the menstrual cycle in female athletes. Method: Saliva samples of the participants (n = 28) were collected successively at 0, 15, 30, and 60 min after awakening to assess cortisol awakening response (CAR) during each of four phases of the menstrual cycle (the menstruation, ovulation, luteal, and premenstrual phases). Diurnal saliva samples were collected at noon, in the evening (17:00) and 22:00 for cortisol analysis. HRV was measured in the menstrual and premenstrual phases at rest (12:00–14:00). Results: There was no difference between CAR parameters in the menstrual phases. CAR parameters and diurnal cortisol levels were significantly correlated with each other throughout the menstrual cycle. Mean cortisol was a strong predictor of the area under the curve (AUC: S = 0.042; R-Sq = 98.4%). Time-dependent parameters of HRV (standard deviation of the N–N interval [SDNN; P = .049] and the root mean square of the successive R–R differences [rMSSD; P = .038]) were significantly higher in the menstrual phase. There were no correlations between CAR and HRV parameters. Conclusions: We suggest that the hypothalamic-pituitary-adrenal axis activity and the autonomic nervous system work in a separate manner that is not affected by the menstrual phases in sportswomen. The present study contributed to a better understanding of the relationship between the dynamics of cortisol release and the autonomic nervous system in different phases of the menstrual cycle in female athletes.

The aim of this study was to investigate the effect of different intensity treadmill exercises on the thiol disulphide homeostasis which is a new marker of oxidative stress in rats. Male albino Wistar rats were randomly divided into four groups as follows: control (CNT), low (LEx), moderate (MEx) and high-intensity exercise (HEx) group. Exercise was performed for 4 weeks. Following completion of the experimental protocol, serum total thiol, native thiol and disulphide concentrations were determined using a novel automated measurement method. Additionally, dynamic disulphide status, reduced thiol, oxidised thiol and thiol oxidation reduction percentage ratios were compared among the groups. Disulphide levels were significantly lower in MEx group and highest in CNT group (p = .047). The lowest oxidised thiol and the highest reduced thiol were determined in CNT group (p = .086; p = .083). These findings indicate that moderate-intensity exercise is more effective in reducing oxidative stress than low and high-intensity exercise.

The study aimed to examine some biochemical test parameters and De Ritis ratio in COVID-19 patients, considering age and gender. The study was performed on patients with real-time polymerase chain ...

The present study aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using thiol/disulphide homeostasis via a novel biomarker in rats. Following the completion of the exercise and l-tyrosine protocol, serum total thiol, native thiol, and disulphide concentrations were determined using a novel automated measurement method. Compared with the control group, serum dynamic disulphide levels were significantly lower in the E group (116.75 ± 10.49; p < .05) and the highest in the LT group (151.0 ± 5.84). The lowest oxidised thiol (49.75 ± 6.18; p = .087) and the highest reduced thiol (75.38 ± 3.16; p = .079) rates were determined to be in the E group. The highest oxidised thiol value was observed in the LT group. Exercise positively affects thiol/disulphide homeostasis, which is a novel indicator of oxidant-antioxidant parameters. Additionally, l-tyrosine appears to be more convenient combined with exercise. The new method used in our study proposes a promising, practical, and useful method for assessing the oxidative stress parameters.

Background: Intestinal microbiota play a role in the health and performance of athletes, and can be influenced by probiotics. Thus, in this study, we aimed to investigate the effect of the use of probiotics combined with chronic exercise on the thiol/disulfide homeostasis, a novel marker of oxidative stress. Methods: Male Wistar rats were randomly divided into four groups: control (Cn), exercise (Ex), probiotics (P), and probiotics + exercise (PEx). A capsule containing 6 × 10⁸ CFU of L. rhamnosus, L. paracasei, L. acidophilus, and B. lactis was given daily for eight weeks to all the experimental animals. The total thiol (TT, μmol/L) and native thiol (NT, μmol/L) concentrations were measured to determine the oxidative stress parameters. The dynamic disulfide (DD, %), reduced thiol (RT, %), oxidized thiol (OT, %), and thiol oxidation reduction (TOR, %) ratios were analyzed. Results: The TT level was found to be significantly higher in the Ex group (p = 0.047, η² = 0.259). The DD level, a marker of oxidation, was significantly lower in the PEx group (p = 0.042, η² = 0.266); the highest value of this parameter was found in the Ex group. The use of probiotics alone had no effect on thiol/disulfide homeostasis. Conclusions: We showed, for the first time, that probiotics administered “with exercise” decreased dynamic disulfide and significantly reduced oxidative damage. Therefore, we speculate that the use of probiotics in sports involving intense exercise might be beneficial to reduce oxidative stress.