Background: Exosomes, widely recognized natural nanovesicles, represent one of the recently discovered modes of intercellular communication due to their ability to transmit crucial cellular information that can be engineered to have robust delivery and targeting capacity. MiR-142-3p, one of the upregulated microRNAs (miRNAs) in many types of breast cancer, activates the canonical Wnt signaling pathway and transactivates the miR-150 expression, and results in the hyperproliferation of cancer cells in vitro and mammary glands in vivo. Materials and methods: In this study, we exploited the exosomes isolated from bone marrow-derived mesenchymal stem cells (MSCs-Exo) to deliver LNA (locked nucleic acid)-modified anti-miR-142-3p oligonucleotides to suppress the expression level of miR-142-3p and miR-150 in 4T1 and TUBO breast cancer cell lines. Results: The in vitro results showed that the MSCs-Exo can efficiently deliver anti-miR-142-3p to reduce the miR-142-3p and miR-150 levels and increase the transcription of the regulatory target genes, APC and P2X7R. We also evaluated in vivo distribution of the MSCs-Exo in tumor-bearing mice. The in vivo result indicated that MSCs-Exo can penetrate the tumor site and are suitable nanovehicles to deliver the inhibitory oligonucleotides into the tumor tissues to downregulate the expression levels of miR-142-3p and miR-150. Conclusion: We showed that MSCs-derived exosomes could be used as a feasible nanovehicle to deliver drug molecules like LNA-anti-miR-142-3p in both in vitro and in vivo studies.

The preparation of silver nanoparticles (Ag-NPs) has been performed through a ‘green’ procedure by utilizing the aqueous flowering shoots of Allium giganteum extract at room temperature. The UV-visible spectrum of Ag-NPs has exhibited an absorbance band at 450 nm. The produced Ag-NPs, in 1.0 mM concentration of AgNO₃, have resulted in the fabrication of spherically shaped nanoparticles with a diameter range of 4.0–35 nm. It has been indicated by the PXRD pattern of Ag-NPs that the bioprepared nanoparticles were crystallized in the fcc crystal construction. Moreover, the green synthesized Ag-NPs seemed to demonstrate a higher antibacterial activity in opposition to pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa), while displaying high toxicity towards cancerous cells (Neuro2A). In accordance to the photocatalytic activity of bioprepared silver nanoparticles, methylene blue (MB) has been perceived to efficiently decrease under UV-lump in the presence of biosynthesized Ag-NPs.