The proliferative response of the neuroblastoma line NB69 to a 100 µT, 50 Hz magnetic field (MF) has been shown mediated by activation of the MAPK-ERK1/2 pathway. This work investigates the MF effect on the cell cycle of NB69, the participation of p38 and c-Jun N-terminal (JNK) kinases in the field-induced proliferative response and the potential involvement of reactive oxygen species (ROS) in the activation of the MAPK-ERK1/2 and -p38 signaling pathways. NB69 cultures were exposed to the 100 µT MF, either intermittently for 24, 42 or 63 h, or continuously for periods of 15 to 120 min, in the presence or absence of p38 or JNK inhibitors: SB203580 and SP600125, respectively. Antioxidant N-acetylcysteine (NAC) was used as ROS scavenger. Field exposure induced transient activation of p38, JNK and ERK1/2. The MF proliferative effect, which was mediated by changes in the cell cycle, was blocked by the p38 inhibitor, but not by the JNK inhibitor. NAC blocked the field effects on cell proliferation and p38 activation, but not those on ERK1/2 activation. The MF-induced proliferative effects are exerted through sequential upregulation of MAPK-p38 and -ERK1/2 activation, and they are likely mediated by a ROS-dependent activation of p38.
Due to the constant updating of regulatory standards on safety issues in electrical installations, limits are established for the maximum step potential that an installation can hold in a ground fault situation. In this paper, an upper bound to the maximum value of the step potentials arising in the soil surface when a fault takes place in a grounded electrical installation is estimated by means of a simple procedure. The direct measurement of the grounding electrode resistance together with some information about the soil resistivity and the knowledge of characteristic parameters of the electrode are used for the calculation of that upper bound. The procedure is tested at numerical simulation level by using different electrodes in several different scenarios corresponding to two-layered soils with different resistivity ratios. The dependency of the calculated upper bound with the electrode burial depth is also studied. Finally, a real case study is presented, and the results of the field measurements are shown as an example of the validity of the procedure.
The possibility of using a set of unequal electrodes, within limits, in a Wenner arrangement for the measurement of apparent resistivity at small depths is explored in this paper. A procedure in which only a simple preliminary calibration is necessary to obtain the best measurements of the apparent resistivity is proposed. On the basis of some case studies, a comparison with the usual procedures to obtain the apparent resistivity from resistance measurements is carried out. The results showed that when an unequal set of electrodes was used, the procedure proposed here was the only one that guaranteed the best apparent resistivity values for any value of electrode separation in the Wenner arrangement, especially for those associated with small depths.
The paper presents a model to include the internal resistance of the grounding electrodes in the calculation of its electrical features. The semi-analytical expressions for the calculation of the grounding resistance arising from the model are used to study the feasibility of the driven-rod method for the estimation of the soil resistivity profile since, unlike other methods, the internal resistance of the conductors can be of great influence for a correct estimate. From the grounding resistance profile an inverse problem based on the minimization of the quadratic differences between the resistance measured and that calculated from the model is posed. Several synthetic examples are used to assess the limitations of the method in conditions close to real situations. Finally, some real cases involving data measured in the field are analyzed. Whether in synthetic examples or in real soils it is found that the spatial frequency of the driven-rod resistance sampling is a determinant factor in order to study the feasibility of the driven–rod method.
22 June 2018 in IET Generation, Transmission & Distribution
The influence of a non‐flat uneven soil surface on the soil apparent resistivity measurements in unidimensional Vertical Electrical Sounding is analysed. The sounding in a real uneven soil surface is simulated by using a soil model, which allows the semi‐analytical expressions valid for perfectly plane soils to be used. In addition, real Wenner electrodes properly calibrated are used, and the differences with a sounding in an equivalent soil with a flat surface are highlighted. Due to the loss of symmetry around the centre of the Wenner array, measurements along different directions are needed and the mean value of the distributions of the measures is assigned to the value of the apparent resistivity. Furthermore, for the purpose of reducing the width of such distribution, the use of electrodes of increasing length is suggested within limits. Finally, the proposal of a multi‐layered soil model with an uneven surface based on the Wenner measurements is discussed.
The measurement of the grounding resistance of grounding grids in large installations as well as grounding electrodes in urban areas is addressed in this article. The resistance value is obtained using a three-pin array by measuring the fall-of-potential on the ground surface. The resistance measured by this method is adjusted to its true value using a correction factor that aligns the measured resistance with the actual value. The proposed measurement method obtains correct values of the grounding resistance even when the auxiliary and potential electrodes of the tree-pin array are close to the electrode to be measured. Thus, it can be applied to large electrodes as well as electrodes in urban areas. Several simulated examples are used to illustrate the method, and some real cases with field measurements are presented for a final validation of the method.