Essential oils (EOs) and organic acids (OAs) are promising feed additives with crucial roles in promoting animal health and performance. This study aimed to assess the combined effects of phytobiotics with organic acids (EOAs) in broiler diets for 39 days. A total of daily 300 male chicks were assigned to three groups, each containing 100 chicks with 5 replicates. The basal diet was supplemented with 0, 0.1, and 0.2% EOAs, respectively. EOAs supplementation did not significantly affect performance values. The villus height/crypt depth ratio in the jejunum and villus dimensions in the ileum showed improvement with EOAs. Dietary EOAs supplementation led to a reduction in the count of Enterobacteriaceae and an increase in Lactobacillus in the caecum. Serum IgA and IgG levels increased with EOAs. Ultimate load, yield load, and the levels of ash, calcium, phosphorus, zinc, and manganese in tibia and femur were higher at high levels of EOAs than in the other groups. In conclusion, the study suggests that 0.1% EOAs usage in diets could be a viable option for enhancing intestinal health, immunity, and bone mineralization as an alternative growth promoter, especially in commercial broiler production.
The present study was undertaken to evaluate the efficacy of 2 graded levels (0.03 and 0.05% of diet) of a monoglyceride blend containing butyric, caprylic, and capric acids in broilers’ diet for optimizing gut structure and animal growth performance. For this purpose, a total of 210, one-day-old male Ross 308 broiler chicks were randomly allocated to 3 experimental treatments using 7 replicates each and 10 birds/replicate. The treatment groups involved supplementation of blend of short and medium chain fatty acids at the level of 0, 0.03, and 0.05% of the diet for 42 d. The incorporation of mixes of monoglycerides into broilers’ diet linearly improved BWG between d 0 and 21 (P = 0.034). At the end of trial, however, no significant changes were observed in performance indexes (BWG, FI, FCR). Jejunal morphometric parameters (villus height, crypt depth, and their ratio) remained unaltered with the monoglyceride supplementation on d 21. The results further showed that monoglycerides supplementation increased the goblet cell counts along the jejunal villi (P = 0.034) and crypt regions (P = 0.022), as well as it effectively modulated the mRNA abundances of tight junction protein (ZO-1, P = 0.033) and nutrient transporters (SGLT, PePT1; P = 0.005, 0.023, respectively) in the jejunum. Moreover, the downregulation in mRNA abundance of TNFα (P = 0.030) was observed with the monoglyceride supplementation. The SCFAs analysis of cecal contents showed no notable differences with monoglyceride blend supplementation when compared to the unsupplemented group. Collectively, high goblet cell numbers in the jejunum along with downregulation of the mRNA abundances of pro-inflammatory cytokines, upregulation of tight junction proteins, and nutrient transporters showed favorable responses of low doses of monoglycerides blend in broiler feeding. Further studies should be conducted in different rearing conditions to examine the effectiveness of such low levels of a monoglyceride blend in the modulation of gut structure, its functionality and animal performance.
Spirulina platensis is a natural antioxidant product that has the ability to improve the performance of poultry. Therefore, the present study aimed to evaluate the effect of using Spirulina platensis as a feed additive in broiler diets. A total of 252 daily male Ross 308 chicks were randomly assigned to six groups. There were two different protein groups: one was at the catalog protein value, and the other was reduced by 10%. Spirulina platensis at 0, 0.1, and 0.2% was added to each protein group. The trial lasted 41 days. Reducing the protein level by 10% had a negative impact on the performance of the chicks. However, Spirulina platensis supplementation had a positive effect on the feed conversion ratio, reduced the oxidative stress index in the chicks’ liver and meat, increased the total antioxidant status and antioxidant enzyme activities, improved the villus height, serum IgG, and some bone parameters, and reduced the serum triglyceride concentration. The carcass yield, visceral organ weight percentages, total phenolic content, and malondialdehyde (MDA) level in the thigh meat and some serum biochemical parameters were not affected by the usage of Spirulina platensis. In conclusion, 0.1% Spirulina platensis could be a feasible feed additive in low-protein diets due to eliciting an improved performance, antioxidant status, and immune response in broilers.
14 September 2021 in Environmental Science and Pollution Research