The effects of fish cannery discharge (FCD) on bacteria in marine coastal sediments were investigated. Redox potentials were measured, and granulometry was determined by wet ASTM sieving, and with the Sedigraph method. Prokaryotic abundance (PA) was determined by epifluorescence microscopy (DAPI staining), and faecal indicator bacteria (FIB) enumerated with the multiple test tube and most probable number method. Total lipids were determined gravimetrically, and sterols analysed by GC/MSD. Bacterial community composition was determined after total DNA isolation, Illumina MiSeq amplification, and SILVAngs processing pipeline. The FCD was rich in lipids, heterotrophic prokaryotes and FIB. The bacterial community of the FCD was dominated by Firmicutes and Gammaproteobacteria and many potentially pathogenic bacteria. Highly porosusgravelly sands clogged with fish remains transitioned to less permeable sandy muds away from the FCD. All sediments were anoxic with extremely negative potentials around the outfall. High surface PA and FIB spread 300 m from the outfall. Gammaproteobacteria and Deltaproteobacteria appeared in all sediments. Sulfurovum and Anaerolineaceae characterized the most polluted locations where gammaproteobacterial Woeseiaceae/JTB255 marine benthic group declined. Gammaproteobacteria and Bacteroidetes characterized surface sediments, while Chloroflexi and Deltaproteobacteria prevailed in deeper layers. The FCD enriched sediments in lipids and allochthonous bacteria degrading sanitary quality, lowering the permeability, redox potential, and bacterial diversity.
The coastal area of the eastern Adriatic is characterized by a prevalence of carbonate rocks and well-developed karst. Present freshwater input into the Adriatic is quite large, mostly through coastal and submarine springs (vruljas). However, there are also a number of rivers debouching in the Adriatic along the eastern coast. Most of them have canyon like fluviokarstic valleys that were carved dominantly during Pleistocene and were drowned during post-Last Glacial Maximum sea-level rise. This caused formation of estuaries which were gradually filled to a different extent during Holocene highstand (last 7.500 years). The intraestuarine delta progradation was rather different in those estuaries depending on the quantity of the river-borne material. Human impact on progradation rate in some of the estuaries has been shown. Formation, évolution et caractéristiques des estuaires karstiques - Exemple de l’AdriatiqueRésumé :La zone côtière de l'Adriatique orientale est caractérisée par une prédominance de roches carbonatées et de karst bien développé. Les apports actuels d'eau douce dans l'Adriatique sont assez importants, principalement à travers les sources côtières et sous-marines (vruljas). Cependant, il y a aussi un certain nombre de rivières qui débouchent dans l'Adriatique le long de la côte orientale. La plupart d'entre elles ont des vallées fluvio-karstiques qui ont été sculptées de façon dominante au cours du Pléistocène et ont été noyées pendant l'élévation maximale du niveau de la mer après le dernier épisode glaciaire. Cela a provoqué la formation d'estuaires qui ont été progressivement remplis à un degré différent au cours de l'Holocène (ces 7.500 dernières années). La progradation du delta intra-estuarien était plutôt différente dans les estuaires en fonction de la quantité de matériel véhiculé par la rivière. L'impact humain sur le taux de progradation dans certains des estuaires a été démontré.Mots clés : Estuaires ; Sédimentation ; Delta intra-estuarien ; Progradation ; Rivière allogénique ; Influence anthropique.