Intercropping with guava (Psidium guajava L.) can assist with the management of Asian citrus psyllid (ACP, Diaphorina citri Kuwayama), the insect vector of the huanglongbing pathogen, in citrus orchards. Sulfur volatiles have a repellent activity and physiological effects, as well as being important components of guava volatiles. In this study, we tested whether the sulfur volatiles emitted by guava plants play a role in plant–plant communications and trigger anti-herbivore activities against ACP in sweet orange plants (Citrus sinensis L. Osbeck). Real-time determination using a proton-transfer-reaction mass spectrometer (PTR-MS) showed that guava plants continuously release methanethiol, dimethyl sulfide (DMS), and dimethyl disulfide (DMDS), and the contents increased rapidly after mechanical damage. The exposure of orange plants to DMDS resulted in the suppression of the developmental performance of ACP. The differential elevation of salicylic acid (SA) levels; the expression of phenylalanine ammonia lyase (PAL), salicylate-O-methyl transferase (SMT), and pathogenesis-related (PR1) genes; the activities of defense-related enzymes PAL, polyphenol oxidase (PPO), and peroxidase (POD); and the total polyphenol content were observed in DMDS-exposed orange plants. The emission of volatiles including myrcene, nonanal, decanal, and methyl salicylate (MeSA) was increased. In addition, phenylpropanoid and flavonoid biosynthesis, and aromatic amino acid (such as phenylalanine, tyrosine, and tryptophan) metabolic pathways were induced. Altogether, our results indicated that DMDS from guava plants can activate defense responses in eavesdropping orange plants and boost their herbivore resistance to ACP, which suggests the possibility of using DMDS as a novel approach for the management of ACP in citrus orchards.

Metarhizium anisopliae, an entomopathogenic fungus, has been widely used for the control of agricultural and forestry pests. However, sporulation degeneration occurs frequently during the process of successive culture, and we currently lack a clear understanding of the underlying mechanisms. In this study, the metabolic profiles of M. anisopliae were comparatively analyzed based on the metabolomics approach of gas chromatography–mass spectrometry (GC–MS). A total of 74 metabolites were detected in both normal and degenerate strains, with 40 differential metabolites contributing significantly to the model. Principal component analysis (PCA) and potential structure discriminant analysis (PLS-DA) showed a clear distinction between the sporulation of normal strains and degenerate strains. Specifically, 23 metabolites were down-regulated and 17 metabolites were up-regulated in degenerate strains compared to normal strains. The KEGG enrichment analysis identified 47 significant pathways. Among them, the alanine, aspartate and glutamate metabolic pathways and the glycine, serine and threonine metabolism had the most significant effects on sporulation, which revealed that significant changes occur in the metabolic phenotypes of strains during sporulation and degeneration processes. Furthermore, our subsequent experiments have substantiated that the addition of amino acids could improve M. anisopliae’s spore production. Our study shows that metabolites, especially amino acids, which are significantly up-regulated or down-regulated during the sporulation and degeneration of M. anisopliae, may be involved in the sporulation process of M. anisopliae, and amino acid metabolism (especially glutamate, aspartate, serine, glycine, arginine and leucine) may be an important part of the sporulation mechanism of M. anisopliae. This study provides a foundation and technical support for rejuvenation and production improvement strategies for M. anisopliae.

The weevil Curculio chinensis Chevrolat (Coleoptera: Curculionidae) is a major cause of economic losses to growers of Camellia oleifera in China, as females lay their eggs in developing fruits and the hatching larvae feed on their seed, aborting fruit growth. Olfactory cues play a key role in the host location of this weevil. The present study focused on identifying volatiles from different parts of the host plant Ca. oleifera, namely, the leaves, fruit peel, and seeds, and testing the antennal and behavioral responses of adult Cu. chinensis to those same volatiles. Methods relied on gas chromatography, electroantennograms, and Y-tube bioassays. The results included a total of twenty-five volatiles emitted by the three plant parts, among which eight elicited antennal responses in Cu. chinensis adults of both sexes. The behavioral bioassays indicated that 3-hexenal, trans-2-hexen-1-ol, methyl salicylate, geraniol, and phenethyl alcohol were attractive to Cu. chinensis, while trans-2-hexenal and 2-ethyl-1-hexanol were repellent. Tests with different concentrations indicated that the behavioral response could be dose-dependent. Future studies should focus on field tests with blends of the attractant compounds in order to develop novel, improved control methods for field applications.