The significance of DNA is no longer limited to its role as a biological information carrier; as a natural polymer, it also become in the field of materials. Single-stranded DNA (ssDNA) molecules with specific sequences can form a G-quadruplex or hairpin-shaped conformation with specific heavy metal ions through coordination bonds. In this study, ssDNA molecules of the four sequences were prepared into hybrid assemblies with one of the famous display materials, the tris-(8-hydroxyquinoline)aluminum (Alq₃) semiconductor. Based on these hybrid assemblies, heavy metal ions, namely Pb²⁺, Hg²⁺, Cd²⁺ and As³⁺, were detected individually at the ppb level. Apart from this, in practical application, many samples containing heavy metal ions are digested with acid. By introducing MES buffer solution, the influence of acidity on the fluorescent signal of Alq₃ was excluded. This strategy showed promising results in the practical application of detecting heavy metal ions in shrub branches and leaves.