Calcium magnesium carbonate nanoparticles (CaMg(CO₃)₂ NPs), well-known as dolomite, are formed by the replacement of half of the calcite minerals of limestone. The dolomite (CaMg(CO₃)₂) nanoparticles are composed of calcite (CaCO₃) and magnesium carbonate (MgCO₃), both of which offer promising strategies for maintaining growth and development in mammals and agricultural plants. A grounded mixture of dolomite limestone was prepared via colloidal precipitates for the synthesis of CaMg(CO₃)₂ NPs, and their characteristics were examined using XRD, particle size analysis by DLS, and surface morphology by SEM and TEM. X-ray photoelectron spectroscopy was used to investigate the binding energy of each element of the dolomite NPs. Spectroscopy revealed that colloidal precipitation is the ideal method for producing NPs. We assessed the numerous beneficial impacts of CaMg(CO₃)₂ NPs in diverse sectors such as agriculture, cancer treatment, and microbiology in this study. Furthermore, an in vivo study was also carried out on chickens to observe the effects of CaMg(CO₃)₂ NPs. The obtained results showed that the treated group with CaMg(CO₃)₂ NPs maintained a more uniform calcium absorption rate than the control group did. The findings of this study suggest that CaMg(CO₃)₂ NPs operate as a stimulant for plants and as an inhibitory agent for bacteria and cancer cells.