The proposed model for getting the score matching of the deoxyribonucleic acid (DNA) sequence is introduced; the Neuro-Fuzzy procedure is the strategy actualized in this paper; it is used the collection of biological information of the DNA sequence performing with global and local calculations so as to advance the ideal arrangement; we utilize the pairwise DNA sequence alignment to gauge the score of the likeness, which depend on information gathering from the pairwise DNA series to be embedded into the implicit framework; an adaptive neuro-fuzzy inference system model is reasonable for foreseeing the matching score through the preparation and testing in neural system and the induction fuzzy system in fuzzy logic that accomplishes the outcome in elite execution.

This paper presented the issues of true representation and a reliable measure for analyzing the DNA base calling is provided. The method implemented dealt with the data set quality in analyzing DNA sequencing, it is investigating solution of the problem of using Neurofuzzy techniques for predicting the confidence value for each base in DNA base calling regarding collecting the data for each base in DNA, and the simulation model of designing the ANFIS contains three subsystems and main system; obtain the three features from the subsystems and in the main system and use the three features to predict the confidence value for each base. This is achieving effective results with high performance in employment.