The ingestion of plastics appears to be widespread throughout the animal kingdom with risks to individuals, ecosystems and human health. Despite growing information on the location, abundance and size distribution of plastics in the environment, it cannot be assumed that any given animal will ingest all sizes of plastic encountered. Here, we use published data to develop an allometric relationship between plastic consumption and animal size to estimate the size distribution of plastics feasibly ingested by animals. Based on more than 2000 gut content analyses from animals ranging over three orders of magnitude in size (lengths 9 mm to 10 m), body length alone accounts for 42% of the variance in the length of plastic an animal may ingest and indicates a size ratio of roughly 20:1 between animal body length and the largest plastic the animal may ingest. We expect this work to improve global assessments of plastic pollution risk by introducing a quantifiable link between animals and the plastics they can ingest.

As the global demand for textiles increases, so to do the potential environmental impacts that stem from their production, use and disposal. Freshwater ecosystems are particularly at risk: rivers often act as the primary recipients of waste generated during the production of textiles and are subject to pollutants released during the broader lifecycle of a textile product. Here, we investigate how global technological and societal processes shape the way we produce, use and dispose of textiles, and what this means for the environmental quality and ecological health of freshwaters. We examine two predominant 'natural' and synthetic textiles (wool and Polyethylene terephthalate (PET), respectively), and find that risks to freshwater ecosystems vary throughout the lifecycle of these textiles; and across geographies, in-line with regulatory and economic landscapes. Woollen textiles pose most risk during the Production Phase, while PET textiles pose most risk during the Use and Disposal Phases. Our findings show that: (i) both 'natural' and synthetic textiles present substantial challenges for freshwater environments; and (ii) bespoke solutions are needed in areas of the world where the global division of labour and less stringent environmental regulations have concentrated textile production; but also in regions where high textile consumption combines with unsustainable disposal behaviours. Effective mitigation may combine technological advances with societal changes in market mechanisms, regulations, textile use and disposal.

Europe's ageing wastewater system often combines domestic sewage with surface runoff and industrial wastewaters. To reduce the associated risk of overloading wastewater treatment works during storms, and to prevent wastewater backing-up into properties, Combined Sewer Overflows (CSOs) are designed into wastewater networks to release excess discharge into rivers or coastal waters without treatment. In view of growing regulatory scrutiny and increasing public concern about their excessive discharge frequencies and potential impacts on environments and people, there is a need to better understand these impacts to allow prioritisation of cost-effective solutions.We review: i) the chemical, physical and biological composition of CSOs discharges; ii) spatio-temporal variations in the quantity, quality and load of overflows spilling into receiving waters; iii) the potential impacts on people, ecosystems and economies. Despite investigations illustrating the discharge frequency of CSOs, data on spill composition and loading of pollutants are too few to reach representative conclusions, particularly for emerging contaminants. Studies appraising impacts are also scarce, especially in contexts where there are multiple stressors affecting receiving waters. Given the costs of addressing CSOs problems, but also the likely long-term gains (e.g. economic stimulation as well as improvements to biodiversity, ecosystem services, public health and wellbeing), we highlight here the need to bolster these evidence gaps. We also advocate no-regrets options to alleviate CSO problems taking into consideration economic costs, carbon neutrality, ecosystem benefit and community well-being. Besides pragmatic, risk-based investment by utilities and local authorities to modernise wastewater systems, these include i) more systemic thinking, linking policy makers, consumers, utilities and regulators, to shift from local CSO issues to integrated catchment solutions with the aim of reducing contributions to wastewater from surface drainage and water consumption; ii) broader societal responsibilities for CSOs, for example through improved regulation, behavioural changes in water consumption and disposal of waste into wastewater networks, and iii) greater cost-sharing of wastewater use.

Anthropogenically forced changes in global freshwater biodiversity demand more efficient monitoring approaches. Consequently, environmental DNA (eDNA) analysis is enabling ecosystem-scale biodiversity assessment, yet the appropriate spatio-temporal resolution of robust biodiversity assessment remains ambiguous. Here, using intensive, spatio-temporal eDNA sampling across space (five rivers in Europe and North America, with an upper range of 20–35 km between samples), time (19 timepoints between 2017 and 2018) and environmental conditions (river flow, pH, conductivity, temperature and rainfall), we characterise the resolution at which information on diversity across the animal kingdom can be gathered from rivers using eDNA. In space, beta diversity was mainly dictated by turnover, on a scale of tens of kilometres, highlighting that diversity measures are not confounded by eDNA from upstream. Fish communities showed nested assemblages along some rivers, coinciding with habitat use. Across time, seasonal life history events, including salmon and eel migration, were detected. Finally, effects of environmental conditions were taxon-specific, reflecting habitat filtering of communities rather than effects on DNA molecules. We conclude that riverine eDNA metabarcoding can measure biodiversity at spatio-temporal scales relevant to species and community ecology, demonstrating its utility in delivering insights into river community ecology during a time of environmental change.

Droughts significantly impact forest ecosystems, reducing forest health and productivity, compromising ecosystem functioning, and nature-based solutions for climate change. The response and resilience of riparian forests to drought are poorly understood despite their key role in the functioning of aquatic and terrestrial ecosystems. Here we investigate riparian forest drought responses and resilience to an extreme drought event at a regional scale. We also examine how drought event characteristics, average climate conditions, topography, soil, vegetation structure, and functional diversity shape the resilience of riparian forests to drought. We used a time series of the Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) to calculate the resistance to and recovery after an extreme drought (2017-2018) in 49 sites across an Atlantic-Mediterranean climate gradient in North Portugal. We used generalized additive models and multi-model inference to understand which factors best explained drought responses. We found a trade-off between drought resistance and recovery (maximum r = -0.5) and contrasting strategies across the climatic gradient of the study area. Riparian forests in the Atlantic regions showed comparatively higher resistance, while Mediterranean forests recovered more. Canopy structure and climate context were the most relevant predictors of resistance and recovery. However, median NDVI and NDWI had not returned to pre-drought levels (Rc_NDWI mean = 1.21, Rc_NDVI mean = 1.01) three years after the event. Our study shows that riparian forests have contrasting drought response strategies and may be susceptible to extended legacy effects associated with extreme and/or recurring droughts, similarly to upland forests. This work highlights the drought vulnerability of riparian ecosystems and emphasises the need for further studies on long-term resilience to droughts.