Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus tristeza virus, and Tomato yellow leaf curl virus have paralyzed the economy of many countries. The cure for major viral diseases is not feasible; however, early detection and surveillance of the disease can obstruct their spread. Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Several rapid nucleic acid diagnostic kits have been developed and validated using Cas9, Cas12, and Cas13 proteins. This review summarizes the CRISPR/Cas-based next-generation molecular diagnostic techniques and portability of devices for field-based utilization.

The yellow mosaic symptoms on soybean (Glycine max) were observed at various sites in the Bundelkhand region of India and infection of begomovirus was detected through PCR. The begomovirus isolates were identified as Mungbean yellow mosaic India virus (MYMIV). MYMIV infection in soybean results in the production of reactive oxygen species to activate defense mechanisms at different levels to overcome the damage caused in the severely infected crops. The biomarkers of stress in plants such as, hydrogen peroxide (H₂O₂), catalase (CAT), Superoxide dismutase (SOD), Ascorbate peroxidase (APX) contents studies also suggested elevation in stress-related enzymes and also a significant reduction in sugar, chlorophyll, and protein contents have been observed. Previously alteration in biochemical due biotic and abiotic stresses have been reported in different varieties of legumes, but biochemical alterations in soybean infected by MYMIV are being reported for the first time from the Bundelkhand region of India.

The Brassica oleracea var. botrytis (cauliflower) is an important annual vegetable crop in the Brassicaceae family and is extensively grown worldwide (Singh et al. 2018). In the early summer of 202...