Porous palladium nanoparticles were designed and synthesized to maximize the pharmacological activity of the chaga mushroom (Inonotus obliquus) extract, which has anticancer and antibacterial activities. In the present study, we synthesized anisotropic porous Pd nanostructures with ultraviolet-visible-near infrared whole wavelength region absorption using chaga extract concentration-dependent reductant-mediated synthesis. The porous Pd nanoparticles exhibited a surface chaga extract-derived anticancer effect, controlled delivery of doxorubicin through electrostatic interaction, and a photothermal conversion effect under 808 nm laser irradiation. The combined application of the three cancer treatment approaches clearly demonstrated the feasibility of synergistic tri-modal therapy. The present platform using Pd, which is a key constituent element of nanocatalysts but is not commonly used in biological applications, suggests numerous applications utilizing Pd nanostructures, as well as the potential development of new cancer therapies.

Chemotherapy, the most commonly applied cancer treatment, often causes unexpected failure due to multidrug resistance (MDR). To overcome MDR, we have designed a platform to realize a combinational synergistic effect of a natural bioactive product (fucoidan), anticancer small compound (doxorubicin), and photothermal nanocarrier (Pt nanoparticle) to treat drug-resistant breast cancer cells. Especially, fucoidan, a sulfated, polysaccharide-structured, therapeutic biopolymer, has been recently recognized as a potential anticancer compound; however, its cancer-inhibiting efficacy has been regarded as low owing to its insufficient level in serum following its conventional oral ingestion. To enhance its potency, fucoidan was applied as a biocompatible surfactant and surface-coating biopolymer in nanocarrier synthesis to manufacture coral-like, fucoidan-coated Pt nanoparticles with a rough surface morphology by a one-pot method. As a result, the biological-thermo-chemo trimodal combination treatment showed excellent therapeutic efficiency against the MDR breast cancer cell MCF-7 ADR both in vitro and in vivo, and the computed tomography contrast effect was also confirmed from the constituent element Pt. Beyond universal application in drug delivery and photothermal therapy, the present approach of applying a MDR modulating/anticancer natural product from nanoparticle synthesis to theranostics will contribute greatly to maximizing their potential through interdisciplinary convergence in the near future.