Background: Black soldier fly (Hermetia illucens) is a promising insect species to use as a novel ingredient in fish feeds. Black soldier fly larvae consists of three major fractions, namely protein, lipid, and exoskeleton. These fractions contain bioactive compounds that can modulate the gut microbiota in fish such as antimicrobial peptides, lauric acid, and chitin. However, it is not certain how, or which fractions of black solider fly would affect gut microbiota in fish. In the present study, black soldier fly larvae were processed into three different meals (full-fat, defatted and de-chitinized) and two fractions (oil and exoskeleton), and included in diets for Atlantic salmon (Salmo salar). Atlantic salmon pre-smolts were fed with these diets in comparison with a commercial-like control diet for eight weeks to investigate the effects of insect meals and fractions on the composition and predicted metabolic capacity of gut microbiota. The gut microbiota was profiled by 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.Results: The inclusion of insect meals and fractions decreased abundance ofProteobacteriaand increased abundance ofFirmicutesin salmon gut. The diets that contained insect chitin, i.e., insect meals or exoskeleton diets, increased abundance of chitinolytic bacteria including lactic acid bacteria andActinomycesin salmon gut, with fish fed full-fat meal diet showing the highest abundances. The diets that contained insect lipids, i.e., insect meals and oil diets enrichedBacillaceaein fish gut. The fish fed diets containing full-fat insect meal had a unique gut microbiota composition dominated by beneficial lactic acid bacteria andActinomyces,and showed a predicted increase in mucin degradation compared to the other diets.Conclusions: The present results showed that the dietary inclusion of insect meals and fractions can differently modulate the composition and predicted metabolic capacity of gut microbiota in Atlantic salmon pre-smolts. The use of full-fat black soldier fly larvae meal in diets for salmon is more favorable for beneficial modulation of gut microbiota than larvae processed by separation of lipid or exoskeleton fractions.

The enzyme NADPH oxidase 1 (NOX1) is a major producer of superoxide which together with other reactive oxygen and nitrogen species (ROS/RNS) are implicated in maintaining a healthy epithelial barrier in the gut. While previous studies have indicated NOX1's involvement in microbial modulation in the small intestine, less is known about the effects of NOX1-dependent ROS/RNS formation in the colon. We investigated the role of NOX1 in the colon of NOX1 knockout (KO) and wild type (WT) mice, under mild and subclinical low-grade colon inflammation induced by 1% dextran sulfate sodium (DSS). Ex vivo imaging of ROS/RNS in the colon revealed that absence of NOX1 strongly decreased ROS/RNS production, particularly during DSS treatment. Furthermore, while absence of NOX1 did not affect disease activity, some markers of inflammation (mRNA: Tnfa, Il6, Ptgs2; protein: lipocalin 2) in the colonic mucosa tended to be higher in NOX1 KO than in WT mice following DSS treatment. Lack of NOX1 also extensively modulated the bacterial community in the colon (16S rRNA gene sequencing), where NOX1 KO mice were characterized mainly by lower α-diversity (richness and evenness), higher abundance of Firmicutes, Akkermansia, and Oscillibacter, and lower abundance of Bacteroidetes and Alistipes. Together, our data suggest that NOX1 is pivotal for colonic ROS/RNS production in mice both during steady-state (i.e., no DSS treatment) and during 1% DSS-induced low-grade inflammation and for modulation of the colonic microbiota, with potential beneficial consequences for intestinal health.

Functional feeds, as a prophylactic strategy, are a promising alternative to address stressful production-related activities (e.g., seawater transfer, delousing) and infectious diseases in salmon farming. To understand the effect of Paecilomyces variotii on short-term growth performance and health responses of vaccinated Atlantic salmon pre-smolts, a control diet (D1) and three experimental diets, where P. variotii replaced 5 % (D2), 10 % (D3) or 20 % (D4) of crude protein, were fed to fish for 28 days in freshwater. Fish fed diets containing P. variotii had a significant dose-dependent linear improvement in feed conversion ratio. Also, D4 group showed a gene expression related to signal truncation and gut homeostasis, while in head kidney (HK), P. variotii activated and controlled immune responses through c-type lectin receptor, downstream signalling molecules (myd88, tollip), cytokines (tnfα, il1β, ifnγ), and effector molecules (cath-2, c3, prx). Moreover, an upregulation of antigen presenting cell markers (mhcii, cd83) and T cell transcriptional factors (gata3, rorc, foxp3) was detected in HK, suggesting that P. variotii could coordinate the innate and adaptive mechanisms. Interestingly, D2 increased specific IgM against Vibrio anguillarum in vaccinated salmon. KEGG analysis revealed that D4 induced decreased abundance of proteins related to inflammatory pathways, e.g., like salmonella infection, apoptosis and necroptosis as well as innate and adaptive signalling pathways in the HK. On the contrary, D4 induced high abundance of proteins related to these inflammatory pathways in the skin mucus (Skm). In addition, complement proteins (i.e., C1q, C4, C7) and arginine metabolism were also in high abundance in the SKm. In relation to the gut microbiota, fish fed D2 and D3 showed low abundance of key lactic acid bacteria (e.g., Weisella, Leuconostoc, Lactobacillus) but high abundance Photobacterium and Ligilactobacillus compared with D1 in the gut. Overall, feed inclusion of P. variotii improved fish growth performance and modulated health response in Atlantic salmon pre-smolts.

Aquaculture is one of the fastest growing food industries worldwide

The effect of dietary graded levels of Cyberlindnera jadinii yeast (C. jadinii) on growth performance, nutrient digestibility, and gut health of broilers was evaluated from 1 to 34 d of age. A total of 360 male broiler chicks were randomly allocated to 1 of 4 dietary treatments (6 replicate pens each) consisting of a wheat-soybean meal-based pelleted diet (Control or CJ0), and 3 diets in which 10% (CJ10), 20% (CJ20), and 30% (CJ30) of the crude protein were supplied by C. jadinii, by gradually replacing protein-rich ingredients. Body weight and feed intake were measured at d 1, 11, 22, and 32. Pellet temperature, durability, and hardness increased linearly (P < 0.05) with C. jadinii inclusion, with highest (P < 0.05) values for CJ30. Up until d 22, feed conversion ratio (FCR) was similar between treatments (P = 0.169). Overall, increasing C. jadinii inclusion linearly increased (P = 0.047) feed intake but had no effect on weight gain or mortality. FCR increased (P < 0.05) linearly with increasing C. jadinii inclusion but only birds fed CJ30 had a significantly poorer FCR compared to the Control. Ileal digestibility was not affected by C. jadinii inclusion, however, there was a significant linear decrease in crude protein and phosphorus, and a tendency for a decrease in fat digestibility. Apparent metabolizable energy (AME) decreased (P < 0.001) quadratically with increasing C. jadinii and was significantly lower in CJ30 compared to the Control. Ileal concentrations of volatile fatty acids (VFAs) were not affected by C. jadinii inclusion, but butyric acid and total VFAs were linearly and quadratically increased and were significantly higher in cecal digesta of birds fed CJ20 and CJ30. Increasing C. jadinii inclusion was associated with an increase (P < 0.05) in the relative abundance of lactobacillus in the ileum and cecum. In conclusion, C. jadinii yeast can supply up to 20% of the total dietary protein without negatively affecting performance, digestibility, or gut health of broilers. The potential confounding role of feed processing and C. jadinii cell wall components on broiler performance is discussed.