Tagetes erecta yaprakları yaklaşık 55°C de 2 saat ısıtıldı ve daha sonra filtre edildi. Ham özüt elde etmek için süzülmüş çözeltinin bir miktarının çözücüsü döner buharlaştırıcı ile uzaklaştırıldı. Diğer filtre edilmiş çözelti, gümüş nitrat çözeltisi ile 60°C da 2 saat etkileştirilerek gümüş nanopartiküller (te-AgNPs) elde edildi. te-AgNPs yapısı spektroskopik yöntemlerle aydınlatıldı. te-AgNPs’ın karakteristik hidroksil titreşimi, Fourier Transform Infrared Spektroskopisi (FTIR) spektrumunda 3214 cm-1 de gözlendi. Ultraviolet-Visible (UV-Vis) spektrofotometre ile maksimim absorbsiyon 422 nm de gözlendi. Taramalı Elektron Mikroskop (SEM) spektral analizi ile de istenilen ürünün ortalama büyüklüğü 46.26 nm olarak belirlendi. X-Işını Kırımı (XRD) spektrumu, te-AgNPs’ın yüzey merkezli kübik kristal yapısında olduğunu ortaya koydu. Özüt ve te-AgNPs örneklerinin antioksidan aktivite testleri yapıldı ve te-AgNPs örneği, 23.80 (IC50, µgml-1), 4.46 (IC50, µgml-1), ve 2.79 (µmolmg-1 örnek) değerleri ile sırasıyla önemli derecede 2,2-Difenil-1-pikrilhidraliz (DPPH), 2,2’-azinobis(3-etilbenzotiazolin-6-sulfonat) (ABTS) ve İndirgeyici Antioksidan Gücü (FRAP) aktivitesi gösterdi.

Schinus molle L. has been used as a folk medicine for many years. The aim of this study is to synthesize the silver nanoparticles, to identify them by spectroscopic techniques and to evaluate the antioxidant effects. In this study, Schinus molle was extracted with hexane, dichloromethane, ethyl acetate and methanol successively. After removal of the solvent from each extract by reduced pressure, the crude extracts were yielded. Methanol extract was used for synthesis of silver nanoparticles (s-AgNPs). The methanol extract dissolved in distilled water was treated with silver nitrate solution to produce the s-AgNPs. The extensive spectroscopic study enabled the identification of synthesized s-AgNPs. UV-Vis spectroscopic analysis presented the maximum absorption of s-AgNPs at 486 nm. The average particle size of s-AgNPs was measured as 77.6 nm by Scanning electron microscope (SEM). X-ray diffraction (XRD) pattern revealed that the nanoparticles were face-centered cubic structure. The slight differences between extract and AgNPs in FTIR spectrum proved the formation of s-AgNPs. In that, some functional group such as hydroxyl in the extract was oxidized while reduction of silver ions. Elemental analysis displayed the silver percentage to be 75.79 %w/w. Antioxidant activity study including DPPH, ABTS•+ and FRAP assays displayed the considerable effect of s-AgNPs. This is the first report to present the synthesis of silver nanoparticles from Schinus molle L. methanol extract and to evaluate the antioxidant activity. HIGHLIGHTS Schinus molle was extracted with hexane, dichloromethane, ethyl acetate and methanol successively Methanol extract was used for synthesis of silver nanoparticles (s-AgNPs) Antioxidant activity study including DPPH, ABTS+and FRAP assays displayed the considerable effect of s-AgNPs GRAPHICAL ABSTRACT

Medicinal plants have been employed commonly in many countries to remedy of various ailments. In this study, Inula graveolens leaves (5.0 g) were extracted with methanol (100 mL) for 24 hours at room temperature. After filtration with Whatman 1 filter pater, the solvent was removed under reduced pressure to yield the crude extract. Quantification of natural products in methanol extract of Inula graveolens was determined by LC-MS/MS. Chlorogenic acid was determined as a chief product (3.5 mg/g extract). Furthermore, salicylic acid (0.055 mg/g extract), caffeic acid (0.054 mg/g extract), protocatechuic acid (0.026 mg/g extract), hesperidin (0.016 mg/g extract), rutin (0.0160 mg/g extract) were found in the methanol extract. Since Inula graveolens contains important bioactive compounds, it has the possibility to be used in pharmaceuticals and food.