Environmental problems associated with marine pollution and climate warming create favorable conditions for the penetration and survival of pathogenic bacteria in marine ecosystems. These microorganisms have interspecific competitive interactions with marine bacteria. Co-culture, as an important research strategy that mimics the natural environment of bacteria, can activate silent genes or clusters through interspecies interactions. The authors used modern biotechnology of co-cultivation to dynamically study intercellular interactions between different taxa of bacteria—pathogenic enterobacteria Yersinia pseudotuberculosis and Listeria monocytogenes and saprotrophic marine bacteria Bacillus sp. and Pseudomonas japonica isolated in summer from the coastal waters of the recreational areas of the Sea of Japan. The results of the experiments showed that during the formation of polycultural biofilms, horizontal transfer of genes encoding some pathogenicity factors from Y. pseudotuberculosis and L. monocytogenes to marine saprotrophic bacteria with different secretion systems is possible. It was previously thought that this was largely prevented by the type VI secretion system (T6SS) found in marine saprotrophic bacteria. The authors showed for the first time the ability of marine bacteria Bacillus sp. and P. japonica to biofilm formation with pathogenic enterobacteria Y. pseudotuberculosis and L. monocytogenes, saprophytic bacteria with type III secretion system (T3SS). For the first time, a marine saprotrophic strain of Bacillus sp. Revealed manifestations of hyaluronidase, proteolytic and hemolytic activity after cultivation in a polycultural biofilm with listeria. Saprotrophic marine bacteria that have acquired virulence factors from pathogenic enterobacteria, including antibiotic resistance genes, could potentially play a role in altering the biological properties of other members of the marine microbial community. In addition, given the possible interdomain nature of intercellular gene translocation, acquired virulence factors can be transferred to marine unicellular and multicellular eukaryotes. The results obtained contribute to the paradigm of the epidemiological significance and potential danger of anthropogenic pollution of marine ecosystems, which creates serious problems for public health and the development of marine culture as an important area of economic activity in coastal regions.

The concentrations of 14 polycyclic aromatic hydrocarbons (PAHs) in the sediment of the Tatar Trough were studied. Despite the increase in PAH concentrations over recent decades, which is likely the result of the handling and transportation of fossil fuels, PAH levels and ecological risk were found to be low. The spatial pattern revealed that higher PAH concentrations were mainly in the deeper water sites, suggesting that trough slope failure transported the PAHs to the deeper part of the basin. There was no correlation between the PAHs and grain size or the PAHs and organic carbon that is related to the PAH input from a variety of sources and the heterogeneity of organic matter. The PAH composition, isomer ratio, and PCA identified two areas with different PAH sources. The most northern part of the Tatar Trough received petrogenic PAHs that are probably transported downslope from the northern Tatar Strait where fossil fuels are handled in some ports. Another trough part was polluted by the PAHs from the combustion of coal and biomass and the exhaust of marine vehicles. The minor presence of genes responsible for aerobic PAH destruction can be explained by the anaerobic degradation of PAHs or the spontaneous creation of favorable conditions that promote bacterial PAH oxidation.

The bioindication of oil and gas fields is a field of geomicrobiology that is mainly devoted to the detection of hydrocarbon-oxidizing microbial indicator species or functional genes in total DNA. However, it appears promising to use the physiological properties of microorganisms detection deposit type of hydrocarbons, in particular their ability to oxidize hydrocarbons under aerobic and anaerobic conditions. In this study, the most promising approach in this area was the method used for assessing the anaerobic degradation of hydrocarbons. When comparing molecular genetics and cultured methods of bioindication, it can be concluded that molecular biomarkers of functional genes for the anaerobic destruction of hydrocarbons (masD) make it possible to separate areas with traditional and gas-hydrate types of deposits. Using cultured methods, we found that representatives of the Nocardiaceae family of the phylum Actinomycetota were tied to the areas where gas hydrates were found. The ability of aerobic and facultative anaerobic hydrocarbon-oxidizing microorganisms to anaerobically utilize hydrocarbons was determined with cultured methods. For the first time, this ability was revealed for the genera Stenotrophomonas, Psychrobacter, Micrococcus and Peribacillus. The wide distribution of this ability that we found in strains isolated from both study regions suggests its prominent role in the destruction of hydrocarbons in marine sediments.

Introduction: After a long period of non-recognition, sapronoses and saprozoonoses have taken their rightful place in the classification of infectious diseases, while the evolutionary features of these infections remain unclear. New facts related to the specifics of their circulation both in the external environment and in warm-blooded animals are revealed and the possibilities of their existence in hydrobionts and plants, as well as formation of uncultivated forms, etc., are being studied. However, recent studies have demonstrated the possibility of microorganisms to co-exist in certain consortia, interacting with other types of microorganisms of other species. In this regard, research on the issue of sapronosis remains sparse. Our objective was to evaluate the ability of saprotrophic bacteria isolated from soil and marine environment to form multicultural biofilms with Listeria monocytogenes, related to sapronoses, and to determine the nature of their interaction in model experiments. Materials and methods: The research was carried out in 2017–2019 using museum strains of different variants of Listeria monocytogenes from the collection of the the Research Institute of Epidemiology and Microbiology named after G.P. Somov and saprophytic bacteria isolated from marine environment and soil. Biofilms were analyzed by spectrophotometry, and the number of viable cells was determined by the number of colony-forming units in 1 ml (CFU/ml). The results showed that the bacteria of a mixed microbial community living in a biofilm could interact with each other through competition or cooperation. Conclusion: Cooperation between different strains of microorganisms in the soil or marine environment may contribute to a better adaptation and survival of L. monocytogenes, thus posing a potential hazard to the population. This fact highlights the epidemiological significance of the marine and soil environments.