Natural deep eutectic solvents (NADESs) coupled with microwave-assisted extraction (MAE) were applied to extract total flavonoid compounds from spent sweet potato (Ipomoea batatas L.) leaves. In this study, ten different NADESs were successfully synthesized for the MAE. Based on single-factor experiments, the response surface methodology (RSM) was applied, and the microwave power, extraction temperature, extraction time, and solid–liquid ratio were further evaluated in order to optimize the yields of total flavonoid compounds. Besides, the extracts were recovered by macroporous resin for the biological activity detection of flavonoid compounds. As a result, NADES-2, synthesized by choline chloride and malic acid (molar ratio 1:2), exhibited the highest extraction yield. After that, the NADES-2-based MAE process was optimized and the optimal conditions were as follows: microwave power of 470 W, extraction temperature of 54 °C, extraction time of 21 min, and solid–liquid ratio of 70 mg/mL. The extraction yield (40.21 ± 0.23 mg rutin equivalents/g sweet potato leaves) of the model validation experiment was demonstrated to be in accordance with the predicted value (40.49 mg rutin equivalents/g sweet potato leaves). In addition, flavonoid compounds were efficiently recovered from NADES-extracts with a high recovery yield (>85%) using AB-8 macroporous resin. The bioactivity experiments in vitro confirmed that total flavonoid compounds had good DPPH and O₂⁻· radical-scavenging activity, as well as inhibitory effects on E. coli, S. aureus, E. carotovora, and B. subtilis. In conclusion, this study provides a green and efficient method to extract flavonoid compounds from spent sweet potato leaves, providing technical support for the development and utilization of sweet potato leaves’ waste.

Organic amendments are widely used to enhance soil fertility and nutrient cycling in greenhouse cultivation, but their effectiveness can vary depending on their origin and composition. This study investigated the impact of four organic materials (rice husk, coconut coir, biochar, and sheep manure) on nutrient cycling and enzyme activities in two of greenhouse tomato soils. The distribution of soil nutrients and enzyme activities was analyzed under controlled conditions during a pot experiment. The addition of organic amendments, regardless of their source, significantly altered the microbial resource allocation, reducing the carbon limitation while increasing the nitrogen demand. The effect on soil nutrient pools was largely determined by the chemical composition of the amendments. In clayey soils, biochar and rice husk additions most effectively promoted enzyme activities related to carbon, nitrogen, and phosphorus acquisition. Under sandy soil conditions, sheep manure increased carbon and phosphorus acquisition enzymes, while biochar most effectively enhanced nitrogen acquisition enzymes. Biochar emerged as a particularly effective amendment, enhancing organic carbon sequestration across different soil types. The chemical composition of the amendments, specifically, the content of carboxyl C, aromatic C, and O-alkyl C, played a crucial role in influencing soil nutrient limitations. In clayey soils, the mean C:N:P ratios for CK, T1, T2, T3, and T4 treatments were 1:1.375:0.625, 1:1.244:0.662, 1:0.839:0.610, 1:1.161:0.689, and 1:1.038:0.549, respectively. In sandy soils, the ratios were 1:1.117:0.698, 1:1.18:0.75, 1:1.096:0.731, 1:1.217:0.689, and 1:1.06:0.669, respectively. These findings suggest that the addition of organic amendments can improve nutrient retention and enzyme activities, but their effects on soil nutrient pools are influenced by both the composition of the amendments and the soil texture. This research enhances our understanding of organic amendments and soil nutrient transformations, and aids in optimizing the application of organic materials for improved soil management in greenhouse cultivation.

Increasing shoot branch numbers of Chrysanthemum morifolium Ramat cv. Hangbaiju (Hangbaiju) is crucial for producing high flower yields. Pot experiments were designed to evaluate the effects of foliar application of 6-benzylaminopurine (6-BA) combined with prohexadione-Ca (Pro-Ca) on the yield and quality of Hangbaiju flowers. Foliar application of 6-BA combined with Pro-Ca typically increased leaf chlorophyll content and decreased leaf soluble sugar and soluble protein contents throughout the floral organ growth phase. At the bud formation phenophase stage (August), the contents of gibberellin (GA), indole-3-acetic acid (IAA), and zeatin (ZA) decreased (except for GA content in the 10 mg L⁻¹ 6-BA combined with 100 mg L⁻¹ Pro-Ca treatment), but abscisic acid (ABA) content increased. The yield of Hangbaiju flowers was found higher in plants treated with foliar application of 6-BA combined with Pro-Ca. Higher yields were found in the 6-BA₅ + Ca₁₀₀ and 6-BA₁₀ + Ca₁₀₀ treatments than in the 6-BA₅ + Ca₅₀ and 6-BA₁₀ + Ca₅₀ treatments, and the highest yield was observed in the 6-Ba₅ + Ca₁₀₀ treatment both in 2019 and 2020. Nutritional indices such as soluble sugar, soluble protein, total amino acid, and water extract increased, and medicinal indices such as flavonoid, total phenolics, chlorogenic acid, cynaroside, and 3,5-dicaffeoyl quinic acid slightly decreased after foliar application of 6-BA combined with Pro-Ca; however, they were all higher than the standards recorded in the Chinese Pharmacopeia. Overall, foliar application of 6-BA combined with Pro-Ca could increase the yield and nutritional quality of Hangbaiju flowers, enhancing its tea quality.

Beneficial microorganisms are an effective approach to ameliorate degraded soils and improve farmland productivity. At present, systematic research on the regulatory effects of plant growth-promoting bacteria on tomato growth in acidic soils is still lacking. In this study, pot experiments combined with microbiome technology were conducted to investigate the effects of Bacillus cereus BC-101, Bacillus methylotrophicus BM-25 and Bacillus amyloliquefaciens BA-11 on the growth of tomato seedlings and the structure of rhizosphere microbial communities in acidic soils. All the three tested plant growth-promoting bacteria significantly promoted the growth of tomato seedlings. The plant heights were increased by 43.01%, 94.38% and 99.20% under BC, BM and BA treatments, respectively. Meanwhile, the bacterial inoculation significantly increased plant biomass, improved leaf photosynthetic pigment contents, activated protective enzyme activities, and elevated the contents of osmotic adjustment substances and growth-promoting hormones. In addition, the bacteria could improve the physicochemical properties of acidic soils, enhance soil enzyme activities, and significantly increase the relative abundances of beneficial genera such as Pseudomonas and Bacillus in the rhizosphere. Rhizospheric inoculation of the three plant growth-promoting bacteria improved tomato growth and enhanced rhizosphere soil properties under naturally acidic soil conditions, suggesting their potential application in the biological improvement of acidic soils. This study provides practical evidence for the comprehensive improvement and application of acidic soils.