High-fat diet (HFD) usually induces oxidative stress and astaxanthin is regarded as an excellent anti-oxidant. An 8-week feeding trial was conducted to investigate the effects of dietary astaxanthin supplementation on growth performance, lipid metabolism, antioxidant ability, and immune response of juvenile largemouth bass (Micropterus salmoides) fed HFD. Four diets were formulated: the control diet (10.87% lipid, C), high-fat diet (18.08% lipid, HF), and HF diet supplemented with 75 and 150 mg kg⁻¹ astaxanthin (HFA1 and HFA2, respectively). Dietary supplementation of astaxanthin improved the growth of fish fed HFD, also decreased hepatosomatic index and intraperitoneal fat ratio of fish fed HFD, while having no effect on body fat. Malondialdehyde content and superoxide dismutase activity were increased in fish fed HFD, astaxanthin supplementation in HFD decreased the oxidative stress of fish. The supplementation of astaxanthin in HFD also reduced the mRNA levels of Caspase 3, Caspase 9, BAD, and IL15. These results suggested that dietary astaxanthin supplementation in HFD improved the growth performance, antioxidant ability and immune response of largemouth bass.

Largemouth bass is an important commercially farmed fish in China, but the rapid expansion of its breeding has resulted in increased incidence of diseases caused by bacteria, viruses and parasites. In this study, moribund largemouth bass containing ulcer foci on body surfaces indicated the most likely pathogens were iridovirus and rhabdovirus members and this was confirmed using a combination of immunohistochemistry, cell culture, electron microscopy and conserved gene sequence analysis. We identified that these fish had been co-infected with these viruses. We observed bullet-shaped virions (100–140 nm long and 50–100 nm in diameter) along with hexagonal virions with 140 nm diameters in cell culture inoculated with tissue homogenates. The viruses were plaque purified and a comparison of the highly conserved regions of the genome of these viruses indicated that they are most similar to largemouth bass virus (LMBV) and hybrid snakehead rhabdovirus (HSHRV), respectively. Regression infection experiments indicated fish mortalities for LMBV-FS2021 and HSHRV-MS2021 were 86.7 and 11.1%, respectively. While co-infection resulted in 93.3% mortality that was significantly (p < 0.05) higher than the single infections even though the viral loads differed by >100-fold. Overall, we simultaneously isolated and identified LMBV and a HSHRV-like virus from diseased largemouth bass, and our results can provide novel ideas for the prevention and treatment of combined virus infection especially in largemouth bass.

This study evaluated the effects of nano-curcumin on growth performance, mucosal immunity, antioxidant response, glucose metabolism, and endoplasmic reticulum stress in largemouth bass. Three groups (three replicates/group) of 270 fish with the mean body weight of 7.00 ± 0.02 g were fed with diets containing 0% (control), 0.1%, and 0.2% nano-curcumin for 60 days. The results showed that dietary supplementation of nano-curcumin had no significant effects on the growth performance, body composition, lysozyme (LYZ), and alkaline phosphatase (AKP) in skin mucus, superoxide dismutase (SOD), and catalase (CAT) activity in serum of largemouth bass. However, dietary supplementation with 0.2% nano-curcumin significantly increased peroxidase (POD) activity in skin mucus and decreased the serum MDA activity compared with the control group. Moreover, dietary supplementation of 0.2% nano-curcumin significantly decreased the levels of serum glucose and liver glycogen, which may be mainly related to the increased gene expression of glucose transporter 2 (GLUT2), glucokinase (GK), phosphofructokinase (PFK), and pyruvate kinase (PK). At the same time, the hepatic gene expression of glucose-regulated protein78 (GRP78), activating transcription factor6 (ATF6), inositol-requiring enzyme 1 (IRE), and eukaryotic initiation factor 2α (eIF2α) in the 0.2% nano-curcumin group were rapidly suppressed, indicating that adding 0.2% nano-curcumin to the diet can alleviate endoplasmic reticulum stress (ERS) in fish. In conclusion, diets supplemented with 0.2% nano-curcumin effectively improved mucosal immunity, antioxidant properties, and glucose metabolism and alleviated ERS induced by long-term carbohydrate intake in largemouth bass.

Microplastics (MPs), which are particles with a diameter of less than 5 mm, have been extensively studied due to their serious global pollution. Typically, MPs in water originate from terrestrial input. A number of studies have reported the presence of MPs as a stressor in water environments worldwide, and their potential threat to the aquatic animals, affecting the growth, oxidative stress responses, body composition, histopathology, intestinal flora, and immune and reproduction systems. During the plastic degradation process, a large variety of toxic substances are released. MPs have been proposed to be the carriers of toxic chemicals and harmful microorganisms. A study of the literature on MP pollution and stress on the aquatic animals associated with MPs was carried out.

The present study investigated the effect of dietary astaxanthin (AX) on the growth performance, antioxidant parameters, and repair of hepatopancreas damage in Pacific white shrimp (Litopenaeus vannamei). To evaluate the hepatopancreas protective function of AX in shrimps, we compared the effect of five isonitrogenous and isoenergetic diets under oxidized fish oil conditions with varying AX levels during the 50-day experimental period. The formulated diets were as follows: (i) OFO (oxidized fish oil); (ii) OFO/AX150 (oxidized fish oil + AX150 mg/kg); (iii) OFO/AX250 (oxidized fish oil + AX250 mg/kg); (iv) OFO/AX450 (oxidized fish oil + AX450 mg/kg); and, (v) control group (fresh fish oil). Results showed that the oxidized fish oil with 275.2 meq/kg peroxide value (POV) resulted in a substantial decrease in the final body weight of L. vannamei (P > 0.05) and induced some visible histopathological alterations in the hepatopancreas. Growth performance was significantly higher in shrimps fed with the OFO/AX450 diet than those fed with the OFO diet (p < 0.05). However, no significant difference was observed when the OFO/AX450 diet was compared to the control diet containing fresh fish oil (p > 0.05). Moreover, shrimps under the OFO/AX450 diet displayed a significant improvement in hepatopancreatic health and showed a reduction of malondialdehyde (MDA) compared to those under the OFO diet (p < 0.05). Dietary AX improved the antioxidant capacity of L. vannamei by increasing the catalase (CAT) activity in the hemolymph. Acute salinity change test showed a higher shrimp survival rate under OFO/AX450 diet than the OFO diet (p < 0.05), suggesting that AX can contribute to enhanced stress tolerance. In conclusion, our data suggest that AX confers dose-dependent protection against OFO-induced oxidative insults and hepatopancreatic damage in shrimp.