Remote sensing is a useful tool for monitoring spatio-temporal variations of crop morphological and physiological status and supporting practices in precision farming. In comparison with multispectral imaging, hyperspectral imaging is a more advanced technique that is capable of acquiring a detailed spectral response of target features. Due to limited accessibility outside of the scientific community, hyperspectral images have not been widely used in precision agriculture. In recent years, different mini-sized and low-cost airborne hyperspectral sensors (e.g., Headwall Micro-Hyperspec, Cubert UHD 185-Firefly) have been developed, and advanced spaceborne hyperspectral sensors have also been or will be launched (e.g., PRISMA, DESIS, EnMAP, HyspIRI). Hyperspectral imaging is becoming more widely available to agricultural applications. Meanwhile, the acquisition, processing, and analysis of hyperspectral imagery still remain a challenging research topic (e.g., large data volume, high data dimensionality, and complex information analysis). It is hence beneficial to conduct a thorough and in-depth review of the hyperspectral imaging technology (e.g., different platforms and sensors), methods available for processing and analyzing hyperspectral information, and recent advances of hyperspectral imaging in agricultural applications. Publications over the past 30 years in hyperspectral imaging technology and applications in agriculture were thus reviewed. The imaging platforms and sensors, together with analytic methods used in the literature, were discussed. Performances of hyperspectral imaging for different applications (e.g., crop biophysical and biochemical properties’ mapping, soil characteristics, and crop classification) were also evaluated. This review is intended to assist agricultural researchers and practitioners to better understand the strengths and limitations of hyperspectral imaging to agricultural applications and promote the adoption of this valuable technology. Recommendations for future hyperspectral imaging research for precision agriculture are also presented.

Multispectral and hyperspectral data have been used to investigate various land cover characteristics. Hyperspectral data have more potential to retrieve information of ground features than multispectral data; however, their limited availability leads to fewer studies in the literature. This research aims to acquire both multispectral and hyperspectral images and compare their performance for estimating vegetation properties (i.e., chlorophyll content). A hyperspectral image (with 325 bands in the visible near infrared (NIR) range) was obtained using a compact hyperspectral sensor mounted on a manned helicopter. A modified camera-based three-band image (with blue, green, and NIR) and a RedEdge sensor-based five-band image (with blue, green, red, red edge, and NIR) were simulated using the hyperspectral image. These three images were compared for the estimation of vegetation chlorophyll content. Partial least square (PLS) regression and random forest regression (RFR) were both applied to estimate chlorophyll using image-derived variables, including vegetation indices and imagery textures. Results show that the RedEdge image achieved good accuracy (R2 ∼ 0.80, RMSE ∼ 14 μg/cm2), close to the accuracy of using the hyperspectral image (R2 ∼ 0.81, RMSE ∼ 13 μg/cm2). The extra bands in the hyperspectral image did not substantially improve chlorophyll estimation. The three-band multispectral image yielded the lowest accuracy (e.g., R2 ∼ 0.42, RMSE ∼ 24 μg/cm2). The RFR performed consistently better than the PLS, owing to its use of randomly-selected training data and predictor variables to build regression trees. These results are expected to provide insights into future studies on the selection of remote sensing images for different monitoring needs.