In Mediterranean ecosystems, the projected rainfall reduction of up to 30% may alter plant–soil interactions, particularly litter decomposition and Home Field Advantage (HFA). We set up a litter transplant experiment in the three main forests encountered in the northern part of the Medi-terranean Basin (dominated by either Quercus ilex, Quercus pubescens, or Pinus halepensis) equipped with a rain exclusion device, allowing an increase in drought either throughout the year or concentrated in spring and summer. Senescent leaves and needles were collected under two precipitation treatments (natural and amplified drought plots) at their “home” forest and were left to decompose in the forest of origin and in other forests under both drought conditions. MS-based metabolomic analysis of litter extracts combined with multivariate data analysis enabled us to detect modifications in the composition of litter specialized metabolites, following amplified drought treatment. Amplified drought altered litter quality and metabolomes, directly slowed down litter decomposition, and induced a loss of home field (dis)advantage. No indirect effect mediated by a change in litter quality on decomposition was observed. These results may suggest major alterations of plant–soil interactions in Mediterranean forests under amplified drought conditions.

Forest ecosystems are some of the largest carbon (C) reservoirs on earth. Pinus halepensis Mill., Quercus ilex L. and Quercus pubescens Willd. represent the dominant tree cover in the Mediterranean forests of southern France. However, their contributions to the French and global forest C and nitrogen (N) stocks are frequently overlooked and inaccurately quantified and little is known about to what extent the ongoing climate change can alter these stocks. We quantified the soil organic C (SOC) and N (SN) stocks in Mediterranean forests dominated by these tree species and evaluated to what extent an experimental precipitation reduction (about −30% yearly) affects these stocks and the litter decomposition efficiency. Litter mass losses were 55.7, 49.8 and 45.7% after 24 months of decomposition in Q. ilex, Q. pubescens and P. halepensis forests, respectively, and were 19% lower under drier climatic conditions. The SOC stocks were 14.0, 16.7 and 18.5 Mg ha⁻¹ and the SN stocks were 0.70, 0.93 and 0.88 Mg ha⁻¹ in Q. ilex, Q. pubescens and P. halepensis forests, respectively. The shallowness and stoniness of these Mediterranean forests could explain these limited stocks. By distinguishing the organic from the organo–mineral layer, we showed 74% less SOC in the organic layer of the P. halepensis forest under drier conditions, while no difference was detected in the organo–mineral layer or in the two oak forests. This last finding deserves further investigation and points out the necessity to distinguish the organic from the organo–mineral layer to detect the first impacts of climate change on SOC stocks.