In this study, the microclimate changes in silvopastoral systems are quantified at different time and spatial scales and compared with single-pasture systems. The study was conducted in a silvopastoral system in São Carlos, Brazil (22°01' South and 47°53' West). The silvopastoral system consisted of grass pastures [Urochloa (sin. Brachiaria) decumbens cv. Basilik] lined with strips of native trees spaced at 17 m. Continuous microclimate measurements (air temperature, relative humidity, wind speed, and incident photosynthetically active radiation) were carried out from September 2010 to September 2012 at two positions of the silvopastoral system (2.0 and 8.5 m from the rows of trees) and at a pasture under full sunlight, located in an adjacent area. The soil moisture was monitored weekly from 24 March 2010 to 1 April 2012 at distances of 0.0, 2.0, 4.75, and 8.5 m from the rows of trees. The rows of native trees reduced wind speeds (46% reduction) and changed the photosynthetically active radiation incidence pattern on the pasture (up to 40% reduction of incidence at the point nearest to the trees). The combined action of these factors promoted thermal and air humidity changes on the pasture at the different points measured. With respect to soil moisture, there was greater soil moisture removal at the sampling points near the rows of native trees under the silvopastoral system, mainly due to enhanced exploration by tree roots at greater depths in dry spells or early dry periods, when compared with the midpoints between the rows.