Myofibrillar proteins (MPs) have a notable impact on the firmness and flexibility of gel-based products. Therefore, enhancing the gelation and emulsification properties of scallop MPs is of paramount significance for producing high-quality scallop surimi products. In this study, we investigated the effects of high-intensity ultrasound on the physicochemical and gelation properties of MPs from bay scallops (Argopecten irradians). The carbonyl content of MPs significantly increased with an increase in ultrasound power (150, 350, and 550 W), indicating ultrasound-induced MP oxidation. Meanwhile, high-intensity ultrasound treatment (550 W) enhanced the emulsifying capacity and the short-term stability of MPs (up to 72.05 m2/g and 153.05 min, respectively). As the ultrasound power increased, the disulfide bond content and surface hydrophobicity of MPs exhibited a notable increase, indicating conformational changes in MPs. Moreover, in the secondary structure of MPs, the α-helix content significantly decreased, whereas the β-sheet content increased, thereby suggesting the ultrasound-induced stretching and flexibility of MP molecules. Sodium-dodecyl sulfate-polyacrylamide gel electrophoresis and scanning electron microscopy analysis further elucidated that high-intensity ultrasound induced MP oxidation, leading to modification of amino acid side chains, intra- and intermolecular cross-linking, and MP aggregation. Consequently, high-intensity ultrasound treatment was found to augment the viscoelasticity, gel strength, and water-holding capacity of MP gels, because ultrasound treatment facilitated the formation of a stable network structure in protein gels. Thus, this study offers theoretical insights into the functional modification of bay scallop MPs and the processing of its surimi products.
31 July 2023 in Journal of the Science of Food and Agriculture
Climate variability has profound effects on vegetation. Spatial distributions of vegetation vulnerability that comprehensively consider vegetation sensitivity and resilience are not well understood in China. Furthermore, the combination of cumulative climate effects and a one-month-lagged autoregressive model represents an advance in the technical approach for calculating vegetation sensitivity. In this study, the spatiotemporal characteristics of vegetation sensitivity to climate variability and vegetation resilience were investigated at seasonal scales. Further analysis explored the spatial distributions of vegetation vulnerability for different regions. The results showed that the spatial distribution pattern of vegetation vulnerability exhibited spatial heterogeneity in China. In spring, vegetation vulnerability values of approximately 0.9 were mainly distributed in northern Xinjiang and northern Inner Mongolia, while low values were scattered in Yunnan Province and the central region of East China. The highest proportion of severe vegetation vulnerability to climate variability was observed in the subhumid zone (28.94%), followed by the arid zone (26.27%). In summer and autumn, the proportions of severe vegetation vulnerability in the arid and humid zones were higher than those in the other climate zones. Regarding different vegetation types, the highest proportions of severe vegetation vulnerability were found in sparse vegetation in different seasons, while the highest proportions of slight vegetation vulnerability were found in croplands in different seasons. In addition, vegetation with high vulnerability is prone to change in Northeast and Southwest China. Although ecological restoration projects have been implemented to increase vegetation cover in northern China, low vegetation resilience and high vulnerability were observed in this region. Most grasslands, which were mainly concentrated on the Qinghai–Tibet Plateau, had high vulnerability. Vegetation areas with low resilience were likely to be degraded in this region. The areas with highly vulnerable vegetation on the Qinghai–Tibet Plateau could function as warning signals of vegetation degradation. Knowledge of spatial patterns of vegetation resilience and vegetation vulnerability will help provide scientific guidance for regional environmental protection.
15 February 2024 in International Journal of Biological Macromolecules
The present work describes the potential application of environmentally friendly sodium carboxymethylcellulose/gelatin (CMC/Gel) semi-interpenetrating hydrogels prepared by citric acid as a nontoxic cross-linking agent to adsorb dyes. The prepared hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA/DTG), and swelling study. The adsorption performance of CMC/Gel2 (C/G2) hydrogel on methylene blue (MB) was investigated. The results showed the better adsorption conditions: adsorption time of 300 min, initial MB concentration of 500 mg/L, adsorbent dosage of 1.2 g/L, solution pH of 7, and temperature of 30 °C. The adsorption kinetics fit the pseudo-second order kinetics model, and the adsorption isotherm fit the Langmuir isotherm model. The maximum adsorption capacity reached 943.40 mg/g. The adsorption process is a spontaneous exothermic process. After three adsorption–desorption cycles, the removal rate of MB by hydrogel still reached 85%, with good reusability. Consequently, the hydrogel can be used as an environmentally friendly, stable, and efficient adsorbent for dyes in wastewater treatment.