This review provides an updated account on the current methods, principles and mechanism of action of therapies for the detection of molecular markers of therapeutic importance in the prognosis of breast cancer progression and recurrence, which includes estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor2 (HER2). Indeed, hormone-receptors namely, ER, PR, proto-oncogene HER2 are the basic molecular markers that are recognized and established prognostic factors and predictors of response, for therapeutic practice. These markers can be detected by using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), which are established, faster and cost effective detection methods. These molecular markers along with clinicopathological prognostic parameters give the best prediction of the prognosis of cancer recurrence and progress. Finally, hormone receptors and HER2 as molecular markers are of prime therapeutic importance and have the capability to take part in future drug development techniques.

Urinary tract infection (UTI) has become a more grievous problem today, due to multidrug resistance of infecting Gram-positive (GP) and Gram-negative (GN) bacteria, sometimes even with multiple infections. This study examines effectivity of 9 tropical flowering plants (Anogeissus acuminata, Azadirachta indica, Bauhinia variegata, Boerhaavia diffusa, Punica granatum, Soymida febrifuga, Terminalia chebula, Tinospora cordifolia and Tribulus terrestris) for possible use as source of antimicrobials for multidrug resistant (MDR) bacteria, along with main-stream antibiotics. Pathogenic bacteria were isolated from urine samples of patients attending and admitted in the hospital. Antibiograms of 11 isolated bacteria (GPs, Enterococcus faecalis and Staphylococcus aureus; and GNs, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris and Pseudomonas aeruginosa) were ascertained by the disc-diffusion method, and antibacterial effectivity of plant extracts was monitored by the agar-well diffusion method. Isolated bacteria were floridly MDR to most antibiotics of the day. Methanol extracts of 9 plants were used, and extracts of 3 plants, A. acuminata, P. granatum and S. febrifuga at least caused 25–29mm as the maximum size of zone of inhibition on bacterial lawns. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of methanol extracts of 9 plants were recorded. The methanol extract of A. acuminata had 0.29mg/ml as the lowest MIC value and 0.67mg/ml as the lowest MBC value, against MDR S. aureus, signifying effectivity; but, it had the highest MIC value of 3.41mg/ml. and the highest MBC value of 4.27mg/ml for most other MDR bacteria including E. coli. Qualitative phytochemical analysis was done for these 9 plants and information on leading phytochemicals was presented retrieved from PubChem database. Thus, three effective-most plants in controlling MDR-UTI bacteria in vitro were A. acuminata, P. granatum and S. febrifuga, which can be promoted as complementary medicine

The burden from microbial diseases has to be addressed in an increasing pace due to multidrug resistance, causing clinical annoyance, globally. Indeed, employing eco-friendly approaches by green syntheses of nanoparticles with metals/metalloids as antibacterial compounds would be continual and cost-effective. Herein, the synthesis of silver nanoparticles (AgNPs) by aqueous extracts of the cyanobacterium Chroococcus minutus (strain, CRLSUM10), collected from the meeting point of sea and river, East coast of India, are presented; both reducing and stabilizing mediators of the synthesized AgNPs were ascertained, by characteriztion by UV–visible spectrophotometry, XRD, FTIR, SEM and SEM-EDX. Furthermore, synthesized AgNPs had a remarkable antibacterial activity at the lowest dose, 100 mg against pathogenic strains of E. coli and S. pyogenes, rendering those as novel antibacterial agents against ‘upper respiratory tract infection’.

The manifestation of bacterial UTI (Urinary Tract Infection) has been predominantly endemic, globally; eventually, the development of new UTI anti-bacterial agent(s) remains the call of the day. Herein, two series of Mannich-based 4-hydroxy coumarin derivatives, 7a-m and 8a-m were designed by suitable heterocyclic amines condensed with aldehydes. The synthesized molecules were interpreted by ¹H and ¹³C NMR spectral analyses with in vitro antibacterial studies. The compound, 4-hydroxy-3-((4-hydroxy-3-methoxyphenyl)(morpholino)methyl)-2H-chromen-2-one 8l was the significant derivative against pathogenic bacteria Staphylococcus aureus and Escherichia coli with MIC values 12.50 and 25µM, respectively. Computational assessments with the Lipinski’s rule of five, ADMET properties and molecular docking studies revealed that analogues, 7f, 7l, 8d, 8j and 8k could be potent druggable molecules with significant binding affinity towards bacterial tyrosine kinase, as target. To understand the mode of binding and intrinsic stabilities of potent receptor-ligand complexes, each system was subjected molecular dynamics simulations for 100 ns. Inter-molecular contact analysis and intrinsic hydrogen-bond stability portrayed the analogues 8l form a number of non-bonded contacts with the receptor tyrosine kinase being mostly dominated by electrostatic and hydrophobic contacts. The results from the present structure-based designing approach might be a valuable tool towards identification of a new antibacterial drug candidate(s) against UTI in near future. List of Abbreviations: 4-HC, 4-hydroxy coumarin; ADMET, absorption, distribution, metabolism, excretion, toxicity; DCE, 1,2-dichloroethane; DCM, dichloromethane; DMSO, dimethyl sulfoxide ; MD, molecular dynamics; MIC, minimum inhibitory concentration; NMR, nuclear magnetic resonance; NPT, isothermal isobaric ensembles; NVT, canonical ensembles; PCA, principal component analysis; PDB, protein data bank; Rg, radius gyration, RMSF, root mean square fluctuations RSMD, root mean square deviation; UTI, Urinary Tract Infection.