Auxin regulates plant growth and development, as well as helps plants to survive abiotic stresses, but the effects of auxin on the growth of alkaline-stressed rice and the underlying molecular and physiological mechanisms remain unknown. Through exogenous application of IAA/TIBA, this study explored the physiological and molecular mechanisms of alkaline stress tolerance enhancement using two rice genotypes. Alkaline stress was observed to damage the plant growth, while exogenous application of IAA mitigates the alkaline-stress-induce inhibition of plant growth. After application of exogenous IAA to alkaline-stressed rice, dry shoot biomass, foliar chlorophyll content, photosynthetic rate in the two rice genotypes increased by 12.6–15.6%, 11.7–40.3%, 51.4–106.6%, respectively. The adventitious root number, root surface area, total root length and dry root biomass in the two rice genotypes increased by 29.3–33.3%, 26.4–27.2%, 42.5–35.5% and 12.8–33.1%, respectively. The accumulation of H₂O₂, MAD were significantly decreased with the application of IAA. The activities of CAT, POD, and SOD in rice plants were significantly increased by exogenous application of IAA. The expression levels of genes controlling IAA biosynthesis and transport were significantly increased, while there were no significant effects on the gene expression that controlled IAA catabolism. These results showed that exogenous application of IAA could mitigate the alkaline-stress-induced inhibition of plant growth by regulating the reactive oxygen species scavenging system, root development and expression of gene involved in IAA biosynthesis, transport and catabolism. These results provide a new direction and empirical basis for improving crop alkaline tolerance with exogenous application of IAA.

Saline–alkaline stress suppresses rice growth and threatens crop production. Despite substantial research on rice’s tolerance to saline–alkaline stress, fewer studies have examined the impact of magnetic water treatments on saline–alkaline-stressed rice plants. We explored the physiological and molecular mechanisms involved in saline–alkaline stress tolerance enhancement via irrigation with magnetized water using Nipponbare. The growth of Nipponbare plants was inhibited by saline–alkaline stress, but this inhibition was alleviated by irrigating the plants with magnetized water, as evidenced by greater plant height, biomass, chlorophyll content, photosynthetic rates, and root system in plants irrigated with magnetized water compared to those irrigated with non-magnetized water. Plants that were irrigated with magnetized water were able to acquire more total nitrogen. In addition, we proved that rice seedlings irrigated with magnetized water had a greater root NO₃⁻-nitrogen concentration and root NH₄⁺-nitrogen concentration than plants irrigated with non-magnetized water. These findings suggest that treatment with magnetized water could increase nitrogen uptake. To test this hypothesis, we analyzed the expression levels of genes involved in nitrogen acquisition. The expression levels of OsNRT1;1, OsNRT1;2, OsNRT2;1, OsAMT1;2, OsAMT2;1, OsAMT2;2, OsAMT2;3, OsAMT3;1, OsAMT3;2, and OsAMT3;3 were higher in plants exposed to magnetized water medium compared to those exposed to non-magnetized water media. We further demonstrated that treatment with magnetized water increases available nitrogen, NO₃⁻-nitrogen content, and NH₄⁺-nitrogen content in soil under saline–alkaline stress. Our results revealed that the increased resistance of rice seedlings to saline–alkaline stress may be attributable to a very effective nitrogen acquisition system enhanced by magnetized water.

Understanding how rainfall is partitioned into throughfall, stemflow, and interception losses by xerophytic trees is important for evaluating afforestation projects and modeling hydrological budgets in semi-arid regions. However, information regarding rainfall partitioning by xerophytic trees and the controlling factors in semi-arid regions remains underrepresented in the literature. We examined whether plant functional groups have a significant impact on rainfall partitioning in two xerophytic trees (evergreen species: Pinus tabuliformis (Pinales:Pinaceae) hereafter called P. tabuliformis, deciduous species: Robinia pseudoacacia L. (Fabales:Fabaceae) hereafter called R. pseudoacacia) commonly used for afforestation on the semi-arid Loess Plateau of China, and evaluated the effects of rainfall, canopy characteristics and meteorological variables on rainfall partitioning. The event-based gross rainfall, throughfall and stemflow were measured during both growing (May–October) and dormant (January–April and November–December) seasons in 2015 and 2016 within an afforested watershed in semi-arid Loess Plateau of China. During our study period, the average rainfall depth for growing season and dormant season was 8.4 mm (varied from 0.2 to 57.6 mm) and 5.6 mm (varied from 0.2 to 41.6 mm), respectively. On average, the measured throughfall, stemflow and interception loss for R. pseudoacacia accounted for 81.8%, 1.4% and 16.8% of gross rainfall, respectively. Corresponding values for P. tabuliformis were 75.1%, 0.7% and 24.1%, respectively. Significant differences (p < 0.05) in stemflow were detected between R. pseudoacacia and P. tabuliformis during both the growing and dormant seasons. The rainfall partitioning components were significantly positively correlated with individual rainfall amounts. The minimum rainfall required to generate stemflow was 5.2 mm for R. pseudoacacia and 5.9 mm for P. tabuliformis during the growing season, and 3.1 mm for R. pseudoacacia and 6.0 mm for P. tabuliformis during the dormant season. Smaller rainfall events contributed to a lower percentage of rainfall amount, throughfall and stemflow but higher percentage of canopy interception loss. The percentage of throughfall and stemflow showed an increased tendency with increasing rain-fall characteristics, while the increasing rainfall characteristics resulted in a decrease in relative interception loss. During the growing season, leaf area index is significantly correlated with throughfall and interception loss of R. pseudoacacia, while there were no significant correlation between meteorological variables and rainfall partitioning. In general, the depth of rainfall partitioning can be predicted reasonably well by using the developed multiple regression models, but the proportions of rainfall partitioning had a relative lower accuracy using the developed models, especially for relative interception loss. To better predict canopy interception loss, other plant morphological and meteorological variables should be considered.

Irrigation guarantee capacity is the critical factor in evaluating the development level of irrigated agriculture and is also a future development trend. It is necessary to carry out scientific planning and reasonable allocation of irrigation water resources to ensure the sustainable development of irrigated agriculture and improve the efficiency and effectiveness of water resource utilization. This study is based on remote sensing meteorological data and the principles of the Miami model and water balance. We calculated the annual irrigation water requirement and effective irrigation water, and used the ratio between the effective irrigation water and irrigation water requirement as the basis for evaluating an irrigation guarantee capability index. By using irrigation guarantee capability evaluation indicators from multiple years, we evaluated and assessed the irrigation guarantee capability in the arid region of northwest China. In addition, we analyzed three indicators (i.e., irrigation water requirement IWR, effective irrigation water EIW, and irrigation guarantee capacity index IGCI) to explore the rational allocation of water resources in the northwest arid area. IWR, EIW, and ICGI in northwest China from 2001 to 2020 were analyzed, and the average values were 379.32 mm, 171.29 mm, and 0.50, respectively. Simultaneously, an analysis was conducted on the temporal and spatial distribution of IWR, EIW, and IGCI in the northwest region of China from 2001 to 2020. The results indicated that the rainfall in the southwestern edge of the Yellow River Basin and the eastern part of the Qaidam Basin could meet the irrigation water demand. The northwest edge of the Yellow River Basin, the central Hexi Inland River Basin, most of Northeast Xinjiang, central and southeastern Xinjiang, and other regions mainly rely on irrigation to meet agricultural water requirements. The rest of the region needs to rely on irrigation for supplementary irrigation to increase crop yield. All districts in the ‘Three Water Lines’ area of northwest China should vigorously develop sprinkler irrigation, micro-irrigation, pipe irrigation, and other irrigation water-saving technologies and support engineering construction. Under the premise of ensuring national food security, they should reduce the planting area of rice, corn, and orchards, and increase the planting area of economic crops such as beans and tubers in the ’Three Water Lines’ area. That is conducive to further reducing the agricultural irrigation quota and improving the matching degree of irrigation water resources. It provides a scientific reference for optimizing water resource allocation and improving irrigation water-use efficiency in northwest arid areas.

Soil water status and fine-root characteristics are the foundation for implementing forest water-management strategies in semiarid forest plantations, where rainwater is always the sole source of water for plant growth. Rainwater management and utilization are effective strategies to alleviate water scarcity in semiarid areas as ground water is always inaccessible there. Through the implementation of an in situ rainwater collection and infiltration system (IRCIS), we investigated the effects of IRCIS on soil water and fine-root distributions in the 0–5 m soil profile in a wet (2015, 815 mm) and a dry year (2016, 468 mm) in rainfed Robinia pseudoacacia forests in the Loess Plateau region of China. The results showed drought significantly decreased plant water availability and hydraulic conductivity of roots and branches, but strongly increased soil moisture deficits and fine-root (<2 mm diameter) biomass. With the implementation of IRCIS, soil profile available water and plant hydraulic conductivity can be significantly increased, but soil moisture deficits and fine-root (1.5 m) soil water in dry years to maintain normal growth and resist drought stress. The results of this study will contribute to the formulation of appropriate strategies for planning and managing rainwater resources in forest plantations.