Salt stress could inhibit the growth and development of crops and negatively affect yield and quality. The objective of this study was to investigate the physiological responses of different asparagus cultivars to salt stress. Twenty days old seedlings ofasalt-tolerant Apollo andasalt-sensitive cultivar JL1 were subjected to 0 (CK) and120 mM NaCl stress for 20 d. Their changes in growth, ion contents, antioxidant enzyme activities and gene expression were analyzed. Salt stress significantly inhibited the growth of both cultivars, and JL1 showed a greater decrease than Apollo. The root development of Apollo was promoted by 120 mM NaCl treatment. The Na⁺ content in roots, stems, and leaves of both cultivars was increased under salt stress, while K⁺ content and K⁺/Na⁺ decreased. The salt-tolerant cultivar Apollo showed less extent of increase in Na⁺ and decrease in K⁺ content and kept a relatively high K⁺/Na⁺ ratio to compare with JL1. The contents of proline, soluble sugar and protein increased in Apollo, while thesesubstances changed differently in JL1 under salt stress. Activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were gradually increased under salt stress in Apollo, while the corresponding enzyme activities in JL1 were decreased at the late stage of salt stress. The expression of SOD, POD, and CAT genes of both cultivars changed in a similar way to the enzyme activities. Malondialdehyde (MDA) content was increased slightly in Apollo, while increased significantly in JL1. At the late stage of salt stress, Apollomaintained a relatively high K⁺/Na⁺, osmotic adjustment ability and antioxidant defense capability, and therefore exhibited higher tolerance to salt stress than that of JL1.

Peanut (Arachis hypogaea L.) is a geocarpic plant, which absorbs nutrients not only through roots but also through pods. However, the effect of nitrogen fertilizer application in pod area on pod gene expression and peanut yield is unknown. In this study, we determined the pod yield, dry matter accumulation, nitrogen accumulation in different organs under different nitrogen treatment in root zone and pod zone. The results showed that pod area application of nitrogen led to significantly higher pod yield to compare with the control. The application of 60 kg/hm² nitrogen at the pod area led to the highest pod yield, full fruit rate and plant nitrogen content. ¹⁵N directly absorbed by pods was mainly accumulated in the shell at early stage of pods development, and then transferred to seeds at later period. However, the ratio of ¹⁵N absorbed directly by pods from soil was low, only about 10%. To understand the underlying molecular mechanisms, immature pods of different developmental stages were collected for gene expression analysis. The expression levels of several genes encoding ABC type transporter family, nitrate reductase, nitrite reductase and glutamine synthetase increased in different stages of pod development upon pod area nitrogen application. These results indicated that the efficiency of N assimilation and glutamate metabolic cycle in pods was increased under pod nitrogen application. The expression levels of several genes in gibberellin and brassinolide biosynthesis pathways were also up-regulated, suggesting that these two hormones were involved in the promoting effect of pod nitrogen application on pod growth.

Ribosome biogenesis is tightly associated with plant growth and reproduction. Mutations in genes encoding ribosomal proteins (RPs) or ribosome biogenesis factors (RBFs) generally result in retarded growth and delayed flowering. However, the early-flowering phenotype resulting from the ribosome biogenesis defect is rarely reported. We previously identified that the AAA-ATPase MIDASIN 1 (MDN1) functions as a 60S RBF in Arabidopsis. Here, we found that its weak mutant mdn1-1 is early-flowering. Transcriptomic analysis showed that the expression of FLOWERING LOCUS C (FLC) is down-regulated, while that of some autonomous pathway genes and ABSCISIC ACID-INSENSITIVE 5 (ABI5) is up-regulated in mdn1-1. Phenotypic analysis revealed that the flowering time of mdn1-1 is severely delayed by increasing FLC expression, suggesting that the early flowering in mdn1-1 is likely associated with the downregulation of FLC. We also found that the photoperiod pathway downstream of CONSTANTS (CO) and FLOWERING LOCUS T (FT) might contribute to the early flowering in mdn1-1. Intriguingly, the abi5-4 allele completely blocks the early flowering in mdn1-1. Collectively, our results indicate that the ribosome biogenesis defect elicited by the mutation of MDN1 leads to early flowering by affecting multiple flowering regulation pathways.