Background: Esophageal cancer is still a leading cause of death among all tumors in males, with unsatisfactory responses to novel immunotherapies such as anti-PD-1 agents. Herein, we explored the role of CD155 in esophageal squamous cell cancer (ESCA) and its underlying molecular mechanisms. Methods: Publicly available datasets were used for differential gene expression and immune infiltration analyses, and their correlation with patient survival. A total of 322 ESCA and 161 paracancer samples were collected and evaluated by performing immunohistochemistry and the H score was obtained by performing semiquantitative analysis. In vitro transfection of ESCA cell lines with lentivirus vectors targeting CD155 was performed to knockdown the protein. These cells were analyzed by conducting RNA sequencing, and the effects of CD155 knockdown on cell cycle and apoptosis were verified with flow cytometry and Western blotting. In addition, in vivo experiments using these engineered cell lines were performed to determine the role of CD155 in tumor formation. A small interfering RNA-mediated knockdown of Nectin3 was used to determine whether it phenocopied the profile of CD155 knockdown. Results: CD155 is highly expressed in ESCA tissues and is positively associated with PD1, PDL1, CD4, IL2RA, and S100A9 expression. Furthermore, CD155 knockdown inhibited ESCA cells’ proliferation by impairing the cell cycle and inducing cell apoptosis. Bioinformatics analysis of the gene expression profile of these engineered cells showed that CD155 mainly contributed to the regulation of PI3K/Akt and MAPK signals. The downregulation of Nectin3 expression phenocopied the profile of CD155 knockdown. Discussion: CD155 may cooperate with PD-1/PD-L1 to support ESCA proliferation in ways other than regulating its underlying immune mechanisms. Indeed, CD155 downregulation can impair ESCA cell pro-cancerous behavior via the inhibition of the PI3K/Akt and MAPK signaling pathways. Moreover, Nectin3 may be a ligand of CD155 and participate in the regulation of ESCA cells’ proliferation. Hence, the inhibition of CD155 may enhance the therapeutic effect of anti-PD-1 immunotherapies in ESCA.

Acute respiratory failure (ARF) with a high incidence among moderate-to-severe traumatic brain injury (M-STBI) patients plays a pivotal role in worsening neurological outcomes. Traumatic subarachnoid hemorrhage (tSAH) is highly prevalent in M-STBI, which is associated with significant adverse outcomes. In this retrospective cohort study, we aimed to explore the association between the severity of the tSAH and ARF in the M-STBI population. A total of 771 subjects were reviewed. Clinical and neuroimaging data of M-STBI patients were retrospectively collected, and ARF was ascertained retrospectively based on their electronic medical record. The degree of tSAH was classified according to Fisher’s criteria, and the grade of tSAH was dichotomized to a low Fisher grade (Fisher grade 1–2) and a high Fisher grade (Fisher grade 3–4). After exclusion procedures, the data of 695 M-STBI patients were analyzed. A total of 284 (30.8%) had a high Fisher grade on admission. The overall rate of ARF within 48 h upon admission was 34.4% (239/695); it was 29.5% (142/481) and 46.3% (99/214) for the low and high Fisher groups, respectively. In a full cohort, a high Fisher grade was associated with ARF after adjusting for age, gender, GCS, smoking history, comorbidities, multiple injuries, characteristics of TBI, and pulmonary factors (OR 1.78; 95% CI, 1.11–2.85, p = 0.016). This result remained robust in the comparisons after PSM (71/132, 42.8% vs. 53/132, 31.9%; OR, 1.59; 95% CI, 1.02–2.49, p = 0.042). A high Fisher SAH grade exposure on admission is associated with ARF in M-STBI patients.

Based on the present global burden of oral diseases, unmet dental needs affect a more significant population worldwide. It is characterized by the need for dental care but receiving delayed or no care. Contributing factors include a lack of knowledge about oral health, its consequences, and the availability of dental services. We need to find out the scale of the problem of unmet dental needs of Chinese male adults. Therefore, the aim was to determine the relationship between the presence of oral conditions and the quality of life-related to oral health. A total of 46,439 Chinese male adults were recruited to the study between August 2022 and October 2023. The questionnaire was administered using standard procedures. This questionnaire sought to gather various types of information, including basic demographic details, oral health status, and specific oral care needs. To gain insight into the frequency and types of oral conditions in our study population, a thorough clinical oral examination was performed. For data interpretation and evaluation, descriptive statistics and Chi square tests were utilized, with a significant level of 5%. The study results indicated that one of the most prevalent oral conditions was oral surface calculus, which was noted in 22,263 (47.94%) participants. The rate of bleeding gums was 6289 (13.54%), with a range of oral diseases such as dental ulcer (1488; 3.2%), trauma (1342; 2.89%), gingivitis (814; 1.75%), pericoronitis (786; 1.69%), and cheilitis (527; 1.13%). The study population reported a 22.41% prevalence of dental caries, with an additional 15.59% of participants having untreated caries. A significant portion of participants (54.62%) expressed a strong need for oral healthcare treatment, with 79.11% self-reporting their need for regular checkups. Furthermore, 81.09% of participants expressed a desire to receive knowledge guidance. Approximately one-fourth of the participants had caries, revealing that age, occupation and location influenced their oral health. Most participants expressed the need for oral treatment, checkups, and knowledge support, stressing the importance of accessibility and availability of oral health services. By specifically targeting age groups and addressing common oral conditions, oral health can be enhanced. Planning strategies should consider demographic factors to maximize impact.