Chitosan and polyethylene glycol (PEG-600) membranes were synthesized and crosslinked with 3-aminopropyltriethoxysilane (APTES). The main purpose of this research work is to synthesize RO membranes which can be used to provide desalinated water for drinking, industrial and agricultural purposes. Hydrogen bonding between chitosan and PEG was confirmed by displacement of the hydroxyl absorption peak at 3237 cm⁻¹ in pure chitosan to lower values in crosslinked membranes by using FTIR. Dynamic mechanical analysis revealed that PEG lowers Tg of the modified membranes vs. pure chitosan from 128.5 °C in control to 120 °C in CS-PEG5. SEM results highlighted porous and anisotropic structure of crosslinked membranes. As the amount of PEG was increased, hydrophilicity of membranes was increased and water absorption increased up to a maximum of 67.34%. Permeation data showed that flux and salt rejection value of the modified membranes was increased up to a maximum of 80% and 40.4%, respectively. Modified films have antibacterial properties against Escherichia coli as compared to control membranes.