Spodoptera frugiperda has caused serious economic damage to various crops. Entomopathogenic nematodes (EPNs) can be used as biological control agents for many pests, including lepidopteran insects. In this study, 218 soil samples were collected from 46 sites in Guangxi, and EPNs were detected in 15 samples. The ITS region of the rDNA gene was used for the molecular identification of isolated nematodes. In total, four and eleven identified populations belonged to Heterorhabditis and Oscheius, respectively. A series of bioassays were conducted to examine the virulence of EPN isolates from Guangxi to control the larvae and pupae of S. frugiperda. The mortality of the third-instar larvae caused by EPNs was concentration dependent. The same dose of EPNs was used to control the third and sixth-instar larvae of S. frugiperda, and the virulence was lower in the sixth-instar larvae. S. frugiperda pupae were treated with different EPNs strains, and the adult eclosion rate of the treated group was significantly lower than that of the sterile water control group (93.3%). Therefore, EPNs could significantly inhibit the eclosion of S. frugiperda pupae. This study provides important information for the biological control of S. frugiperda with EPNs.

Thirteen new species of the genus Stathmopoda are described from China: S. atrifusca sp. nov., S. cellifaria sp. nov., S. cornuta sp. nov., S. digitiprocessa sp. nov., S. dolichantha sp. nov., S. falsistimulata sp. nov., S. guangxiensis sp. nov., S. ingena sp. nov., S. liberata sp. nov., S. purpurata sp. nov., S. serrifasciaria sp. nov., S. similatridorsalis sp. nov. and S. spinicornuta sp. nov. Images of both adults and genitalia of the new species are provided, along with a map showing the distribution of these species.

Ten new species of the genus Stathmopoda are described: S. basirotata sp. nov., S. bicoloriptera sp. nov., S. bucera sp. nov., S. hamulata sp. nov., S. ochricolorata sp. nov., S. octacantha sp. nov., S. pyriformis sp. nov., S. rhombica sp. nov., S. tetracantha sp. nov. and S. tristriata sp. nov. Also, three species: S. citrinella Sinev, 1995, S. orbiculata Meyrick, 1913 and S. tecticochlea Terada, 2016 are newly recorded for China. Images of both adults and genitalia for the species are provided, along with a map showing the distribution of them in China.

Fourteen new species of the genus Stathmopoda Herrich-Schäffer, 1853 are described: S. apicihamata S. Wang & Guan, sp. nov., S. sphaeroidea S. Wang & Guan, sp. nov., S. delitescens S. Wang & Guan, sp. nov., S. ferrorufa S. Wang & Guan, sp. nov., S. jinxiuensis S. Wang & Guan, sp. nov., S. laiyangensis S. Wang & Guan, sp. nov., S. miniloba S. Wang & Guan, sp. nov., S. paradiplaspis S. Wang & Guan, sp. nov., S. similignominiosa S. Wang & Guan, sp. nov., S. paraxanthostigma S. Wang & Guan, sp. nov., S. sufusciumeraris S. Wang & Guan, sp. nov., S. trigonia S. Wang & Guan, sp. nov., S. trilobata S. Wang & Guan, sp. nov. and S. xanthostigma S. Wang & Guan, sp. nov.; ten species are newly recorded for China: S. aprica Meyrick, 1913, S. balanarcha Meyrick, 1921, S. callicarpicola Terada, 2012, S. cissota Meyrick, 1913, S. culcitella Sinev, 1995, S. diplaspis (Meyrick, 1887), S. flavescens Kuznetzov, 1984, S. gemmiconsuta Terada, 2012, S. placida Meyrick, 1908 and S. xanthomochla Meyrick, 1913. Images of both adults and genitalia are provided, along with two maps showing the distribution of them in China.